Cloned (Comment) | Organism |
---|---|
pcpB gene, expression of wild-type and mutant in Escherichia coli as glutathione-S-transferase fusion protein | Flavobacterium sp. |
Protein Variants | Comment | Organism |
---|---|---|
additional information | introduction of tobacco etch virus protease cleavage site into the enzyme as a glutathione-S-transferase fusion protein | Flavobacterium sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
Pentachlorophenol | recombinant enzyme | Flavobacterium sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Flavobacterium sp. | - |
identical with Sphingomonas chlorophenolica strain ATCC 39723 | - |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant from Escherichia coli as glutathione-S-transferase fusion proteins | Flavobacterium sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pentachlorophenol + NADPH + H+ + O2 | - |
Flavobacterium sp. | 2,3,5,6-tetrachlorohydroquinone + NADP+ + chloride + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PCP4MO | - |
Flavobacterium sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | recombinant enzyme has no FAD, activity only in presence of added FAD | Flavobacterium sp. |