Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.14.13.50 extracted from

  • Leung, K.T.; Campbell, S.; Gan, Y.; White, D.C.; Lee, H.; Trevors, J.T.
    The role of the Sphingomonas species UG30 pentachlorophenol-4-monooxygenase in p-nitrophenol degradation (1999), FEMS Microbiol. Lett., 173, 247-253.
    View publication on PubMed
Search for more literature for 1.14.13.50

Cloned(Commentary)

Cloned (Comment) Organism
-
 Flavobacterium sp.
pcpB gene, expression in Escherichia coli BL21, DNA sequence analysis Sphingomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
59930
-
 deduced from DNA sequence determination Sphingomonas sp.

Organism

Organism UniProt Comment Textmining
Flavobacterium sp.
-
 identical with Sphingomonas chlorophenolica strain ATCC 39723
-
Sphingomonas sp. O68977 UG30
-
Sphingomonas sp. UG30 O68977 UG30
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Flavobacterium sp.
additional information
-
-
 Sphingomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrocatechol + NADPH + O2
-
 Sphingomonas sp. ?
-
 ?
4-nitrocatechol + NADPH + O2
-
 Sphingomonas sp. UG30 ?
-
 ?
additional information broad substrate specificity, hydroxylation in para-position Flavobacterium sp. ?
-
 ?
additional information broad substrate specificity, hydroxylation in para-position Sphingomonas sp. ?
-
 ?
additional information broad substrate specificity, hydroxylation in para-position Sphingomonas sp. UG30 ?
-
 ?
p-nitrophenol + NADPH + O2 low activity Sphingomonas sp. ?
-
 ?
p-nitrophenol + NADPH + O2 low activity Sphingomonas sp. UG30 ?
-
 ?
pentachlorophenol + NADPH + H+ + O2
-
 Flavobacterium sp. 2,3,5,6-tetrachlorohydroquinone + NADP+ + chloride + H2O
-
 ?
pentachlorophenol + NADPH + H+ + O2
-
 Sphingomonas sp. 2,3,5,6-tetrachlorohydroquinone + NADP+ + chloride + H2O
-
 ?
pentachlorophenol + NADPH + H+ + O2
-
 Sphingomonas sp. UG30 2,3,5,6-tetrachlorohydroquinone + NADP+ + chloride + H2O
-
 ?

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Flavobacterium sp.
FAD highly conserved FAD-binding site at the N-terminus Sphingomonas sp.
NADPH
-
 Flavobacterium sp.
NADPH
-
 Sphingomonas sp.