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Literature summary for 1.14.13.44 extracted from

  • Jensen, C.N.; Mielke, T.; Farrugia, J.E.; Frank, A.; Man, H.; Hart, S.; Turkenburg, J.P.; Grogan, G.
    Structures of the Apo and FAD-bound forms of 2-hydroxybiphenyl 3-monooxygenase (HbpA) locate activity hotspots identified by using directed evolution (2015), ChemBioChem, 16, 968-976 .
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21 Pseudomonas nitroreducens

Crystallization (Commentary)

Crystallization (Comment) Organism
single crystals of the enzyme are used to determine its structure in two forms: an FAD-bound form that allows characterisation of the active site, and an apo form that, although lacking flavin, gives extra information on the location of residues and structure of mobile loops that are absent from the FAD complex Pseudomonas nitroreducens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0031
-
 2-Hydroxybiphenyl pH 7.5, temperature not specified in the publication, mutant enzyme H48A Pseudomonas nitroreducens
0.0033
-
 2-Hydroxybiphenyl pH 7.5, temperature not specified in the publication, wild-type enzyme Pseudomonas nitroreducens
0.0048
-
 2-Hydroxybiphenyl pH 7.5, temperature not specified in the publication R242A Pseudomonas nitroreducens
0.005
-
 2-Hydroxybiphenyl pH 7.5, temperature not specified in the publication, mutant enzyme D117A Pseudomonas nitroreducens

Organism

Organism UniProt Comment Textmining
Pseudomonas nitroreducens O06647
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas nitroreducens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-hydroxybiphenyl + NADH + H+ + O2
-
 Pseudomonas nitroreducens 2,3-dihydroxybiphenyl + NAD+ + H2O
-
 ?

Subunits

Subunits Comment Organism
tetramer the structure of the tetramer is determined, there is a large cavity at the centre of the tetramer into which the side chains of several hydrophilic amino acids of each monomer, including Glu75 and Tyr76, are projected Pseudomonas nitroreducens

Synonyms

Synonyms Comment Organism
HbpA
-
 Pseudomonas nitroreducens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Pseudomonas nitroreducens

Cofactor

Cofactor Comment Organism Structure
FAD FAD-dependent monooxygenase Pseudomonas nitroreducens