Cloned (Comment) | Organism |
---|---|
gene nos1, quantitative reverse-transcription PCR enzyme expression analysis | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-NG-monomethyl arginine | L-NMMA | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 L-arginine + 3 NADPH + 3 H+ + 4 O2 | Rattus norvegicus | overall reaction | 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O | - |
? | |
2 L-arginine + 3 NADPH + 3 H+ + 4 O2 | Rattus norvegicus Sprague Dawley | overall reaction | 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O | - |
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Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | P29476 | - |
- |
Rattus norvegicus Sprague Dawley | P29476 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
dental papilla | dental papilla cells (DPCs) are mesenchymal cells that are surrounded by the enamel organ during tooth development | Rattus norvegicus | - |
additional information | NO synthase (NOS) immunohistochemic analysis and nicotinamide adenine dinucleotide phosphate-diaphorase (NADPH-d) staining in vivo in rat odontoblasts. The expression of neuronal NOS and endothelial NOS is upregulated during the odontoblastic differentiation of DPCs | Rattus norvegicus | - |
odontoblast | from rats at different ages. Odontoblasts are strongly stained for NADPH-d. The staining intensity of odontoblasts near the future cusp and root is higher than that of other locations of the first molar. Odontoblasts in the first molar from 3-month-old rats are also positive for NADPH-d staining. Odontoblasts in the first molar from 8-month-old rats are weakly positive for NADPH-d staining. Semiquantitative evaluation results of NADPH-d staining suggests that NOS may be involved in the differentiation and function of odontoblasts | Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 L-arginine + 3 NADPH + 3 H+ + 4 O2 | overall reaction | Rattus norvegicus | 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O | - |
? | |
2 L-arginine + 3 NADPH + 3 H+ + 4 O2 | overall reaction | Rattus norvegicus Sprague Dawley | 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
NADPH-d | - |
Rattus norvegicus |
nicotinamide adenine dinucleotide phosphate-diaphorase | - |
Rattus norvegicus |
NO synthase | - |
Rattus norvegicus |
NOS | - |
Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Rattus norvegicus |
Organism | Comment | Expression |
---|---|---|
Rattus norvegicus | expression of neuronal NOS and endothelial NOS is upregulated during the odontoblastic differentiation of dental papilla cells (DPCs) | up |
General Information | Comment | Organism |
---|---|---|
malfunction | inhibition of NOS function by NOS inhibitor L-NG-monomethyl arginine (L-NMMA) results in reduced formation of mineralized nodules and expression of dentin sialophosphoprotein (DSPP) and dentin matrix protein (DMP1) during dental papilla cell (DPC) differentiation | Rattus norvegicus |
physiological function | nitric oxide (NO) regulates the functions of multiple cells and organ tissues, including stem cell differentiation and bone formation, role of nitric oxide in odontoblastic differentiation of rat dental papilla cells, overview. NO regulates the odontoblastic differentiation of dental papilla cells (DPCs), thereby influencing dentin formation and tooth development. The NO donor S-nitroso-N-acetylpenicillamine (SNAP) promotes the viability of DPCs. Extracellular matrix mineralization and odontogenic markers expression are elevated by SNAP at low concentrations and suppressed at high concentration. Blocking the generation of cyclic guanosine monophosphate (cGMP) with 1H-(1,2,4)oxadiazolo-(4,3-a)quinoxalin-1-one (ODQ) abolishes the positive influence of SNAP on the odontoblastic differentiation of DPCs | Rattus norvegicus |