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Literature summary for 1.14.13.39 extracted from

  • Li, H.; Das, A.; Sibhatu, H.; Jamal, J.; Sligar, S.G.; Poulos, T.L.
    Exploring the electron transfer properties of neuronal nitric-oxide synthase by reversal of the FMN redox potential (2008), J. Biol. Chem., 283, 34762-34772.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.13.39

Cloned(Commentary)

Cloned (Comment) Organism
the wild type and the DELTAG810 mutant of nNOS are cloned in Escherichia coli strain DH5alpha Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
additional information the DELTAG810 mutant enzyme shows strongly reduced activity towards nitric oxide synthesis and NADPH oxidation compared to the wild type enzyme Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus P29476
-
-

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 L-arginine + 3 NADPH + 3 H+ + 4 O2
-
 Rattus norvegicus 2 citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O
-
 ?

Synonyms

Synonyms Comment Organism
neuronal nitric-oxide synthase
-
 Rattus norvegicus
nNOS
-
 Rattus norvegicus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.033
-
 nitric oxide DELTAG810 mutant enzyme, in 25 mM potassium phosphate, pH 7.5, with 100 mM KCl, at 25°C Rattus norvegicus
0.055
-
 nitric oxide DELTAG810 mutant enzyme, in the presence of 10 units/ml catalase and superoxide dismutase, in 25 mM potassium phosphate, pH 7.5, with 100 mM KCl, at 25°C Rattus norvegicus
0.28
-
 NADPH DELTAG810 mutant enzyme, in the presence of 10 units/ml catalase and superoxide dismutase, in 25 mM potassium phosphate, pH 7.5, with 100 mM KCl, at 25°C Rattus norvegicus
0.32
-
 NADPH DELTAG810 mutant enzyme, in 25 mM potassium phosphate, pH 7.5, with 100 mM KCl, at 25°C Rattus norvegicus
0.92
-
 nitric oxide wild type enzyme, in 25 mM potassium phosphate, pH 7.5, with 100 mM KCl, at 25°C Rattus norvegicus
1.23
-
 nitric oxide wild type enzyme, in the presence of 10 units/ml catalase and superoxide dismutase, in 25 mM potassium phosphate, pH 7.5, with 100 mM KCl, at 25°C Rattus norvegicus
2.33
-
 NADPH wild type enzyme, in the presence of 10 units/ml catalase and superoxide dismutase, in 25 mM potassium phosphate, pH 7.5, with 100 mM KCl, at 25°C Rattus norvegicus
2.57
-
 NADPH wild type enzyme, in 25 mM potassium phosphate, pH 7.5, with 100 mM KCl, at 25°C Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
5,6,7,8-tetrahydro-L-biopterin
-
 Rattus norvegicus
Calmodulin
-
 Rattus norvegicus
NADPH
-
 Rattus norvegicus