Cloned (Comment) | Organism |
---|---|
expression of endothelial isoform III in Spodoptera frugiperda cells via baculovirus infection | Bos taurus |
General Stability | Organism |
---|---|
tetrahydrobiopterin stabilizes dimeric form | Bos taurus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
diphenylene iodonium | inhibition of superoxide production of recombinant isoform III | Bos taurus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 L-arginine + 3 NADPH + 4 O2 + 3 H+ | Bos taurus | - |
2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O | - |
? | |
additional information | Bos taurus | enzyme shows also superoxide formation activity | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant endothelial isoform III from insect cells | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
endothelium | isoform III | Bos taurus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 L-arginine + 3 NADPH + 4 O2 + 3 H+ | - |
Bos taurus | 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O | - |
? | |
additional information | enzyme shows also superoxide formation activity, uneffected by L-arginine, inhibited by tetrahydrobiopterin and diphenyleneiodonium | Bos taurus | ? | - |
? | |
additional information | enzyme shows also superoxide formation activity | Bos taurus | ? | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
5,6,7,8-tetrahydro-L-biopterin | stimulates | Bos taurus | |
FAD | major source of superoxide production in absence of tetrahydrobiopterin | Bos taurus | |
NADPH | - |
Bos taurus |