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Literature summary for 1.14.13.39 extracted from

  • Mayer, B.; John, M.; Böhme, E.
    Purification of a Ca2+/calmodulin-dependent nitric oxide synthase from porcine cerebellum. Cofactor-role of tetrahydrobiopterin (1990), FEBS Lett., 277, 215-219.
    View publication on PubMed
Search for more literature for 1.14.13.39

General Stability

General Stability Organism
glycerol stabilizes Sus scrofa

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activation only in the presence of calmodulin Sus scrofa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
160000
-
 x * 160000, SDS-PAGE Sus scrofa
200000
-
 above, gel filtration Sus scrofa

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation (30% saturation), 2',5'-ADP-affinity chromatography Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
 Sus scrofa
-
cerebellum
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.73
-
 purified enzyme Sus scrofa

Storage Stability

Storage Stability Organism
-70°C, with 20% v/v glycerol at least 4 weeks stable Sus scrofa
4°C, unstable Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 L-arginine + 3 NADPH + 4 O2 + 3 H+
-
 Sus scrofa 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O
-
 ?

Subunits

Subunits Comment Organism
? x * 160000, SDS-PAGE Sus scrofa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Sus scrofa

Cofactor

Cofactor Comment Organism Structure
5,6,7,8-tetrahydro-L-biopterin
-
 Sus scrofa
NADPH
-
 Sus scrofa