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Literature summary for 1.14.13.39 extracted from

  • Hevel, J.M.; White, K.A.; Marletta, M.A.
    Purification of the inducible murine macrophage nitric oxide synthase. Identification as a flavoprotein (1991), J. Biol. Chem., 266, 22789-22791.
    View publication on PubMed

General Stability

General Stability Organism
freezing inactivates, 50% v/v glycerol protects Mus musculus
glycerol stabilizes Mus musculus
Mg2+ does not stabilize Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.014 0.018 L-arginine
-
Mus musculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
130000
-
2 * 130000, SDS-PAGE Mus musculus
260000
-
gel filtration Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus
-
cytokine-inducible in macrophage
-

Purification (Commentary)

Purification (Comment) Organism
inducible form from macrophage Mus musculus
sequential anion-exchange-, affinity chromatogaphy Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
macrophage
-
Mus musculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.62
-
purified enzyme Mus musculus

Storage Stability

Storage Stability Organism
-80°C, stable overnight in 50% v/v glycerol Mus musculus
4°C, t1/2: 2 h Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 L-arginine + 3 NADPH + 4 O2 + 3 H+
-
Mus musculus 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O
-
?

Subunits

Subunits Comment Organism
dimer 2 * 130000, SDS-PAGE Mus musculus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Mus musculus

Cofactor

Cofactor Comment Organism Structure
5,6,7,8-tetrahydro-L-biopterin presumably tightly enzyme-bound Mus musculus
FAD 1 mol per mol of enzyme subunit Mus musculus
FAD FAD containing flavoprotein Mus musculus
FAD slight activation by exogeneous FAD Mus musculus
FMN 1 mol per mol of enzyme subunit Mus musculus
FMN FMN containing flavoprotein Mus musculus
NADPH
-
Mus musculus