Literature summary for 1.14.13.38 extracted from

Peric-Concha, N.; Borovicka, B.; Long, P.F.; Hranueli, D.; Waterman, P.G.; Hunter, I.S.
Ablation of the otcC gene encoding a post-polyketide hydroxylase from the oxytetracyline biosynthetic pathway in Streptomyces rimosus results in novel polyketides with altered chain length (2005), J. Biol. Chem., 280, 37455-37460.

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Cloned(Commentary)

Cloned (Comment)	Organism
gene otcC, DNA and amino acid sequence determination and analysis, expression of the glycine mutant enzyme in Escherichia coli strain BL21(DE3)	Streptomyces rimosus

Protein Variants

Protein Variants	Comment	Organism
additional information	a otcC disruption mutant strain of Streptomyces rimosus synthesizes a novel C-17 polyketide, the ability to make a 19-carbon backbone in the mutant strain is restored when the inactive mutant enzyme, with three essential glycine residues of the NADH-binding domain mutated by site-directed mutagenesis, is expressed in the disruption mutant strain, thus the quarternary structure of the enzyme is required, not only the its activity, in the synthase complex	Streptomyces rimosus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
anhydrotetracycline + NADPH + O2	Streptomyces rimosus	the enzyme catalyzes a hydroxylation of the anthracycline structure at position C-6 after biosynthesis of the polyketide backbone is completed, Biosynthesis of oxytetracycline, overview	12-dehydrotetracycline + NADP+ + H2O	product identification	?

Organism

Organism	UniProt	Comment	Textmining
Streptomyces rimosus	Q58PK7	gene otcC, strain R6-500	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.0000112	-	recombinant wild-type enzyme in Escherichia coli cell extract	Streptomyces rimosus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
anhydrotetracycline + NADPH + O2	-	Streptomyces rimosus	12-dehydrotetracycline + NADP+ + H2O	-	?
anhydrotetracycline + NADPH + O2	the enzyme catalyzes a hydroxylation of the anthracycline structure at position C-6 after biosynthesis of the polyketide backbone is completed, Biosynthesis of oxytetracycline, overview	Streptomyces rimosus	12-dehydrotetracycline + NADP+ + H2O	product identification	?

Synonyms

Synonyms	Comment	Organism
anhydrotetracycline oxygenase	-	Streptomyces rimosus

Cofactor

Cofactor	Comment	Organism	Structure
NADPH	three essential glycine codons are located within the putative NADPH-binding domain	Streptomyces rimosus

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Release 2023.1 (January 2023)