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Literature summary for 1.14.13.251 extracted from

  • Shao, Y.; Guo, L.; Zhang, Y.; Yu, H.; Zhao, B.; Pang, H.; Lu, W.
    Glycine betaine monooxygenase, an unusual Riesketype oxygenase system, catalyzes the oxidative N-demethylation of glycine betaine in Chromohalobacter salexigens DSM 3043 (2018), Appl. Environ. Microbiol., 84, e00377-18 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.13.251

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Chromohalobacter salexigens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0439
-
 NADH cosubstrate FAD, pH 7,25°C Chromohalobacter salexigens
0.258
-
 NADPH cosubstrate FAD, pH 7,25°C Chromohalobacter salexigens

Metals/Ions

Metals/Ions Comment Organism Structure
Iron contents of iron and acid-labile sulfide in the purified enzyme are 1.88 mol and 1.76 mol per mol BmoB Chromohalobacter salexigens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45100
-
 subunit BmoB, gel filtration Chromohalobacter salexigens
154800
-
 subunit BmoA, gel filtration Chromohalobacter salexigens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
glycine betaine + NADH + H+ + O2 Chromohalobacter salexigens
-
 N,N-dimethylglycine + formaldehyde + NAD+ + H2O
-
 ?
glycine betaine + NADH + H+ + O2 Chromohalobacter salexigens DSM 3043
-
 N,N-dimethylglycine + formaldehyde + NAD+ + H2O
-
 ?
glycine betaine + NADPH + H+ + O2 Chromohalobacter salexigens
-
 N,N-dimethylglycine + formaldehyde + NADP+ + H2O
-
 ?
glycine betaine + NADPH + H+ + O2 Chromohalobacter salexigens DSM 3043
-
 N,N-dimethylglycine + formaldehyde + NADP+ + H2O
-
 ?
additional information Chromohalobacter salexigens FMN is the most favored electron acceptor for BmoB compared with FAD and riboflavin ?
-
-
additional information Chromohalobacter salexigens DSM 3043 FMN is the most favored electron acceptor for BmoB compared with FAD and riboflavin ?
-
-

Organism

Organism UniProt Comment Textmining
Chromohalobacter salexigens Q1QYU6 and Q1QYU7 Q1QYU6 i.e. oxidoreductase FAD-binding protein BmoB, Q1QYU7 i.e. Rieske (2Fe-2S) protein BmoA
-
Chromohalobacter salexigens DSM 3043 Q1QYU6 and Q1QYU7 Q1QYU6 i.e. oxidoreductase FAD-binding protein BmoB, Q1QYU7 i.e. Rieske (2Fe-2S) protein BmoA
-

Storage Stability

Storage Stability Organism
BmoA-His6 solution is very unstable and maintains no more than 10% residual activity when stored for 2 weeks at -40°C in elution buffer with no addition of enzyme stabilizer Chromohalobacter salexigens
His6-BmoB is stable for at least 2 weeks when stored at -40°C in elution buffer with no addition of enzyme stabilizer Chromohalobacter salexigens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glycine betaine + NADH + H+ + O2
-
 Chromohalobacter salexigens N,N-dimethylglycine + formaldehyde + NAD+ + H2O
-
 ?
glycine betaine + NADH + H+ + O2
-
 Chromohalobacter salexigens DSM 3043 N,N-dimethylglycine + formaldehyde + NAD+ + H2O
-
 ?
glycine betaine + NADPH + H+ + O2
-
 Chromohalobacter salexigens N,N-dimethylglycine + formaldehyde + NADP+ + H2O
-
 ?
glycine betaine + NADPH + H+ + O2
-
 Chromohalobacter salexigens DSM 3043 N,N-dimethylglycine + formaldehyde + NADP+ + H2O
-
 ?
additional information FMN is the most favored electron acceptor for BmoB compared with FAD and riboflavin Chromohalobacter salexigens ?
-
-
additional information FMN is the most favored electron acceptor for BmoB compared with FAD and riboflavin Chromohalobacter salexigens DSM 3043 ?
-
-

Subunits

Subunits Comment Organism
tetramer 3 * 52500, subunit BmoA, plus 1 * 47700, subunit BmoB, SDS-PAGE Chromohalobacter salexigens

Synonyms

Synonyms Comment Organism
BmoA
-
 Chromohalobacter salexigens
BmoB
-
 Chromohalobacter salexigens
Csal_1004
-
 Chromohalobacter salexigens
Csal_1005
-
 Chromohalobacter salexigens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.35
-
 NADPH cosubstrate FAD, pH 7,25°C Chromohalobacter salexigens
0.9
-
 NADH cosubstrate FAD, pH 7,25°C Chromohalobacter salexigens

Cofactor

Cofactor Comment Organism Structure
FAD each BmoB monomer contains one FAD molecule Chromohalobacter salexigens
NAD+
-
 Chromohalobacter salexigens
NADH NADH is the preferred electron donor over NADPH Chromohalobacter salexigens
NADPH NADH is the preferred electron donor over NADPH Chromohalobacter salexigens

General Information

General Information Comment Organism
evolution BmoA belongs to group V of the Rieske nonheme iron oxygenase family Chromohalobacter salexigens
metabolism in the presence of BmoB, NADH, and flavin, BmoA can aerobically degrade glycine betaine to dimethylglycine with the concomitant production of formaldehyde. BmoA exhibits strict substrate specificity for glycine betaine, and its demethylation activity is stimulated by Fe2+ Chromohalobacter salexigens
physiological function enzyme is involved in a degradation pathway of glycine betaine. Escherichia coli expressing Bmoa/BmoB accumulates dimethylglycine. BmoB is an NADH-dependent flavin reductase with one noncovalently bound flavin adenine dinucleotide (FAD) as its prosthetic group Chromohalobacter salexigens