Literature summary for 1.14.13.25 extracted from

Li, Y.; Wang, Y.; Lin, Z.; Wang, J.; He, Q.; Zhou, J.
A novel methanotrophic co-metabolic system with high soluble methane monooxygenase activity to biodegrade refractory organics in pulping wastewater (2018), Biores. Technol., 256, 358-365 .

Search for more literature for 1.14.13.25

Application

Application	Comment	Organism
environmental protection	pulping wastewater still contains massive refractory organics after biotreatment, with high colority, low biodegradability, and lasting biotoxicity. To eliminate refractory organics in pulping wastewater, a methanotrophic co-metabolic system in a gas cycle Sequencing Batch Biofilm Reactor (gcSBBR) seeded by soil at a ventilation opening of coal mine is quickly built on the 92nd day. The removal rate of COD, colority and TOC is 53.28%, 50.59% and 51.60%, respectively. Analysis of 3D-EEM indicates that glycolated protein-like, melanoidin-like or lignocellulose-like, and humic acid-like decrease by 7.85%, 5.02% and 1.74%, respectively	Methylosinus trichosporium

Cloned(Commentary)

Cloned (Comment)	Organism
gene mmoX, quantitative RT-PCR enzyme expression analysis	Methylosinus trichosporium

Protein Variants

Protein Variants	Comment	Organism
additional information	establishing of a methanotrophic co-metabolic system, built in the gcSBBR seeded by soil at a ventilation opening of coal mine in the presence of Cu2+, and seeded with sMMO in Methylosinus trichosporium OB3b and particulate methane monooxygenase (pMMO) in Methylocystis parvus in a biofilm, microbial community analyses and microbial 16S rRNA sequencing, and postulated pathway of methanotrophic co-metabolic system, overview. Six kinds of methanotrophs are detected from the seeded soil, namely, Methylocystis (0.29%), Methylocystaceae_unclassified (0.16%), Methylocystaceae_uncultured (0.04%) and Methylocaldum (0.14%), Methylococcaceae_unclassified (0.10%), Methylobacteriaceae_uncultured (0.04%), totally accounting for 0.77%	Methylosinus trichosporium

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
methane + NAD(P)H + H+ + O2	Methylosinus trichosporium	-	methanol + NAD(P)+ + H2O	-	?

Organism

Organism	UniProt	Comment	Textmining
Methylosinus trichosporium	A0A2D2D5X0 AND A0A2D2D0T8 AND Q53563 AND A0A2D2D0X7	MMOH, MMOR, MMOB, and MMOD	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
methane + NAD(P)H + H+ + O2	-	Methylosinus trichosporium	methanol + NAD(P)+ + H2O	-	?
additional information	a colorimetric assay is adopted for the sMMO activity detection of biofilm	Methylosinus trichosporium	?	-	-

Synonyms

Synonyms	Comment	Organism
sMMO	-	Methylosinus trichosporium
soluble methane monooxygenase	-	Methylosinus trichosporium

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	assay at	Methylosinus trichosporium

Cofactor

Cofactor	Comment	Organism	Structure
FAD	-	Methylosinus trichosporium
NAD(P)H	-	Methylosinus trichosporium

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Release 2023.1 (January 2023)