Protein Variants | Comment | Organism |
---|---|---|
T111Y | mutant enzyme with increased rate constant for the reaction of large substrates such as ethane, furan, and nitrobenzene with the reactive MMOH (regulatory componant of the enzyme) intermediate Q while decreasing the rate constant for the reaction with methane. The regiospecificity for nitrobenzene oxidation is altered and 10fold more T111Y than wild-type MMOB is required to maximize the rate of turnover | Methylosinus trichosporium |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Methylosinus trichosporium | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methylosinus trichosporium | - |
OB3b | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ethane + NADH + O2 | - |
Methylosinus trichosporium | ethanol + NAD+ + H2O | - |
? | |
furan + NADH + O2 | - |
Methylosinus trichosporium | ? + NAD+ + H2O | - |
? | |
methane + NADH + O2 | - |
Methylosinus trichosporium | methanol + NAD+ + H2O | - |
? | |
nitrobenzene + NADH + O2 | - |
Methylosinus trichosporium | nitrophenol + NAD+ + H2O | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Methylosinus trichosporium |