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Literature summary for 1.14.13.25 extracted from

  • Brazeau, B.J.; Lipscomb, J.D.
    Key amino acid residues in the regulation of soluble methane monooxygenase catalysis by component B (2003), Biochemistry, 42, 5618-5631.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
T111Y mutant enzyme with increased rate constant for the reaction of large substrates such as ethane, furan, and nitrobenzene with the reactive MMOH (regulatory componant of the enzyme) intermediate Q while decreasing the rate constant for the reaction with methane. The regiospecificity for nitrobenzene oxidation is altered and 10fold more T111Y than wild-type MMOB is required to maximize the rate of turnover Methylosinus trichosporium

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
Methylosinus trichosporium
-
-

Organism

Organism UniProt Comment Textmining
Methylosinus trichosporium
-
OB3b
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ethane + NADH + O2
-
Methylosinus trichosporium ethanol + NAD+ + H2O
-
?
furan + NADH + O2
-
Methylosinus trichosporium ? + NAD+ + H2O
-
?
methane + NADH + O2
-
Methylosinus trichosporium methanol + NAD+ + H2O
-
?
nitrobenzene + NADH + O2
-
Methylosinus trichosporium nitrophenol + NAD+ + H2O
-
?

Cofactor

Cofactor Comment Organism Structure
NADH
-
Methylosinus trichosporium