Cloned (Comment) | Organism |
---|---|
expression of component protein B of sMMO as glutathione-S-transferase fusion protein in Escherichia coli | Methylococcus capsulatus |
Protein Variants | Comment | Organism |
---|---|---|
G13Q | enhanced temperature stability compared to wild-type, site-directed mutagenesis | Methylococcus capsulatus |
G13Q | sMMO, alteration of a cleavage site in component protein B | Methylococcus capsulatus |
additional information | native parallel occurence of full length and 2 N-terminal truncated forms of regulatory component protein B of sMMO, truncated forms are inactive | Methylococcus capsulatus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | cytoplasmatic, soluble enzyme form termed sMMO | Methylococcus capsulatus | 5737 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
15850 | - |
component protein B of sMMO, mass spectrometry | Methylococcus capsulatus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methylococcus capsulatus | - |
enzyme form sMMO | - |
Purification (Comment) | Organism |
---|---|
recombinant component protein B of sMMO as glutathione-S-transferase fusion protein from Escherichia coli | Methylococcus capsulatus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
recombinant shows higher activity than the wild-type | Methylococcus capsulatus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
methane + NAD(P)H + O2 | - |
Methylococcus capsulatus | methanol + NAD(P)+ + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MMO | - |
Methylococcus capsulatus |
pMMO | particulate, membrane-bound enzyme form | Methylococcus capsulatus |
sMMO | soluble, cytoplasmic enzyme form | Methylococcus capsulatus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Methylococcus capsulatus |