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Literature summary for 1.14.13.247 extracted from

  • Daughtry, K.; Xiao, Y.; Stoner-Ma, D.; Cho, E.; Orville, A.; Liu, P.; Allen, K.
    Quaternary ammonium oxidative demethylation X-ray crystallographic, resonance Raman, and UV-visible spectroscopic analysis of a Rieske-type demethylase (2012), J. Am. Chem. Soc., 134, 2823-2834 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.13.247

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Sinorhizobium meliloti

Crystallization (Commentary)

Crystallization (Comment) Organism
in complex with stachydrine, hanging drop vapor diffusion method, using 15% (w/v) poly(ethylene glycol) 3350, 20% (v/v) glycerol, 100 mM succinic acid buffer, pH 7.0, and 10 mM hexamine cobalt at 22°C Sinorhizobium meliloti

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
stachydrine + NADH + H+ + O2 Sinorhizobium meliloti
-
 N-methyl-L-proline + formaldehyde + NAD+ + H2O
-
 ?
stachydrine + NADPH + H+ + O2 Sinorhizobium meliloti
-
 N-methyl-L-proline + formaldehyde + NADP+ + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Sinorhizobium meliloti
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Strep-Tactin resin column chromatography Sinorhizobium meliloti

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no activity with N,N-dimethyl-glycine, N-methyl-glycine, and N-methyl-L-proline Sinorhizobium meliloti ?
-
-
stachydrine + NADH + H+ + O2
-
 Sinorhizobium meliloti N-methyl-L-proline + formaldehyde + NAD+ + H2O
-
 ?
stachydrine + NADPH + H+ + O2
-
 Sinorhizobium meliloti N-methyl-L-proline + formaldehyde + NADP+ + H2O
-
 ?

Synonyms

Synonyms Comment Organism
stachydrine demethylase
-
 Sinorhizobium meliloti
stc2
-
 Sinorhizobium meliloti

Cofactor

Cofactor Comment Organism Structure
additional information non-heme enzyme Sinorhizobium meliloti
NADH
-
 Sinorhizobium meliloti
NADPH
-
 Sinorhizobium meliloti
[2Fe-2S]-center
-
 Sinorhizobium meliloti