Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Burkholderia cepacia |
Protein Variants | Comment | Organism |
---|---|---|
A113H | mutation in subunit TomA3. Mutant enzyme produces primarily isatin from indole | Burkholderia cepacia |
A113I | mutation in subunit TomA3. Mutant enzyme produces primarily indirubin from indole | Burkholderia cepacia |
A113V | mutation in subunit TomA3, colony turns blue. Mutant enzyme produces primarily indigo from indole | Burkholderia cepacia |
V106A/A113G | mutation in subunit TomA3. Mutant enzyme produces primarily isatin from indole | Burkholderia cepacia |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Burkholderia cepacia | Q9ANX4 and Q9ANX0 and Q9ANX3 and Q8VSV8 and Q9ANX1 and Q8VSV9 | Q9ANX4 i.e. subunit tomA1, Q9ANX0 i.e. subunit tomA5, Q9ANX3 i.e. subunit TomA2, Q8VSV8 i.e. subunit TomA3, Q9ANX1 i.e. subunit TomA4, Q8VSV9 i.e. subunit TomA0 | - |
Burkholderia cepacia G4 | Q9ANX4 and Q9ANX0 and Q9ANX3 and Q8VSV8 and Q9ANX1 and Q8VSV9 | Q9ANX4 i.e. subunit tomA1, Q9ANX0 i.e. subunit tomA5, Q9ANX3 i.e. subunit TomA2, Q8VSV8 i.e. subunit TomA3, Q9ANX1 i.e. subunit TomA4, Q8VSV9 i.e. subunit TomA0 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
indole + NADH + H+ + O2 | - |
Burkholderia cepacia | isoindigo + NAD+ + H2O | - |
? | |
indole + NADH + H+ + O2 | - |
Burkholderia cepacia G4 | isoindigo + NAD+ + H2O | - |
? | |
additional information | wild-type forms isoindigo via C-2 hydroxylation of the indole pyrrole. Mutant TomA3 A113G produces 4-hydroxyindole. TomA3 V106/A113 mutants with hydrophobic, polar, or charged amino acids hydroxylate indole at the C-3 and C-2 positions, forming isatin, indigo, and indirubin in a variety of distributions | Burkholderia cepacia | ? | - |
? | |
additional information | wild-type forms isoindigo via C-2 hydroxylation of the indole pyrrole. Mutant TomA3 A113G produces 4-hydroxyindole. TomA3 V106/A113 mutants with hydrophobic, polar, or charged amino acids hydroxylate indole at the C-3 and C-2 positions, forming isatin, indigo, and indirubin in a variety of distributions | Burkholderia cepacia G4 | ? | - |
? |