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Literature summary for 1.14.13.242 extracted from

  • Lazar, J.T.; Shuvalova, L.; Rosas-Lemus, M.; Kiryukhina, O.; Satchell, K.J.F.; Minasov, G.
    Structural comparison of p-hydroxybenzoate hydroxylase (PobA) from Pseudomonas putida with PobA from other Pseudomonas spp. and other monooxygenases (2019), Acta Crystallogr. Sect. F, 75, 507-514 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.13.242

Crystallization (Commentary)

Crystallization (Comment) Organism
crystal structure analysis, PDB ID 5hxi, comparison to the structure of HbpA from Pseudomonas nitroreducens strain HBP1 (PDB ID 4cy8) Mesorhizobium japonicum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-hydroxy-2-methylpyridine-5-carboxylate + NAD(P)H + H+ + O2 Mesorhizobium japonicum
-
 2-(acetamidomethylidene)succinate + NAD(P)+
-
 ?
3-hydroxy-2-methylpyridine-5-carboxylate + NAD(P)H + H+ + O2 Mesorhizobium japonicum LMG 29417
-
 2-(acetamidomethylidene)succinate + NAD(P)+
-
 ?
3-hydroxy-2-methylpyridine-5-carboxylate + NAD(P)H + H+ + O2 Mesorhizobium japonicum CECT 9101
-
 2-(acetamidomethylidene)succinate + NAD(P)+
-
 ?

Organism

Organism UniProt Comment Textmining
Mesorhizobium japonicum Q988D3
-
-
Mesorhizobium japonicum CECT 9101 Q988D3
-
-
Mesorhizobium japonicum LMG 29417 Q988D3
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-hydroxy-2-methylpyridine-5-carboxylate + NAD(P)H + H+ + O2
-
 Mesorhizobium japonicum 2-(acetamidomethylidene)succinate + NAD(P)+
-
 ?
3-hydroxy-2-methylpyridine-5-carboxylate + NAD(P)H + H+ + O2
-
 Mesorhizobium japonicum LMG 29417 2-(acetamidomethylidene)succinate + NAD(P)+
-
 ?
3-hydroxy-2-methylpyridine-5-carboxylate + NAD(P)H + H+ + O2
-
 Mesorhizobium japonicum CECT 9101 2-(acetamidomethylidene)succinate + NAD(P)+
-
 ?
additional information comparisons of substrate binding structure analysis mechanism Mesorhizobium japonicum ?
-
-
additional information comparisons of substrate binding structure analysis mechanism Mesorhizobium japonicum LMG 29417 ?
-
-
additional information comparisons of substrate binding structure analysis mechanism Mesorhizobium japonicum CECT 9101 ?
-
-

Synonyms

Synonyms Comment Organism
2-methyl-3-hydroxypyridine-5-carboxylic acid oxygenase
-
 Mesorhizobium japonicum
MHPCO
-
 Mesorhizobium japonicum
mlr6788
-
 Mesorhizobium japonicum

Cofactor

Cofactor Comment Organism Structure
FAD binding domain structure comparisons, overview Mesorhizobium japonicum
NADH
-
 Mesorhizobium japonicum

General Information

General Information Comment Organism
additional information sequence comparisons, three-dimensional enzyme structure analysis, and structure comparisons with 2-hydroxybiphenyl 3-monooxygenase (HbpA) from Pseudomonas nitroreducens and 2-methyl-3-hydroxypyridine-5-carboxylic acid oxygenase (MHPCO, PDB ID 5hxi) from Mesorhizobium japonicum, overview. Despite having only 14% similarity in their primary sequences, pairwise structure alignments of PobA from Pseudomonas putida with HbpA from Pseudomonas nitroreducens and MHPCO from Mesorhizobium japonicum reveal local similarities between these structures. Key residues in the FAD-binding and substrate-binding sites of PobA are highly conserved spatially across the proteins from all three species. The PobA from Pseudomonas putida is structurally very similar to PobA from Pseudomonas fluorescens and from Pseudomonas aeruginosa. Key secondary-structure elements important for catalysis, such as the betaalphabeta fold, beta-sheet wall and alpha12 helix, are conserved across this expanded class of oxygenases Mesorhizobium japonicum
physiological function 2-methyl-3-hydroxypyridine-5-carboxylic acid oxygenase (MHPCO) is a flavoenzyme that catalyzes oxidative ring opening of 2-methyl-3-hydroxypyridine-5-carboxylic acid Mesorhizobium japonicum