BRENDA - Enzyme Database
show all sequences of 1.14.13.239

Carnitine metabolism to trimethylamine by an unusual Rieske-type oxygenase from human microbiota

Zhu, Y.; Jameson, E.; Crosatti, M.; Schaefer, H.; Rajakumar, K.; Bugg, T.D.; Chen, Y.; Proc. Natl. Acad. Sci. USA 111, 4268-4273 (2014)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli BLR(DE3) pLysS cells
Acinetobacter baumannii
Engineering
Amino acid exchange
Commentary
Organism
E205A
inactive
Acinetobacter baumannii
E205D
inactive
Acinetobacter baumannii
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-carnitine + NADH + H+ + O2
Acinetobacter baumannii
-
(3R)-3-hydroxy-4-oxobutanoate + trimethylamine + NAD+ + H2O
-
-
?
L-carnitine + NADH + H+ + O2
Acinetobacter baumannii ATCC19606
-
(3R)-3-hydroxy-4-oxobutanoate + trimethylamine + NAD+ + H2O
-
-
?
L-carnitine + NADPH + H+ + O2
Acinetobacter baumannii
-
(3R)-3-hydroxy-4-oxobutanoate + trimethylamine + NADP+ + H2O
-
-
?
L-carnitine + NADPH + H+ + O2
Acinetobacter baumannii ATCC19606
-
(3R)-3-hydroxy-4-oxobutanoate + trimethylamine + NADP+ + H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Acinetobacter baumannii
D0C9N6 AND D0C9N8
alpha subunit and beta subunit
-
Acinetobacter baumannii ATCC19606
D0C9N6 AND D0C9N8
alpha subunit and beta subunit
-
Purification (Commentary)
Commentary
Organism
His-tag affinity column chromatography
Acinetobacter baumannii
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-carnitine + NADH + H+ + O2
-
746297
Acinetobacter baumannii
(3R)-3-hydroxy-4-oxobutanoate + trimethylamine + NAD+ + H2O
-
-
-
?
L-carnitine + NADH + H+ + O2
-
746297
Acinetobacter baumannii ATCC19606
(3R)-3-hydroxy-4-oxobutanoate + trimethylamine + NAD+ + H2O
-
-
-
?
L-carnitine + NADPH + H+ + O2
-
746297
Acinetobacter baumannii
(3R)-3-hydroxy-4-oxobutanoate + trimethylamine + NADP+ + H2O
-
-
-
?
L-carnitine + NADPH + H+ + O2
-
746297
Acinetobacter baumannii ATCC19606
(3R)-3-hydroxy-4-oxobutanoate + trimethylamine + NADP+ + H2O
-
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
FAD
-
Acinetobacter baumannii
NAD+
-
Acinetobacter baumannii
NADH
-
Acinetobacter baumannii
NADPH
-
Acinetobacter baumannii
[2Fe-2S]-center
-
Acinetobacter baumannii
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli BLR(DE3) pLysS cells
Acinetobacter baumannii
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
FAD
-
Acinetobacter baumannii
NAD+
-
Acinetobacter baumannii
NADH
-
Acinetobacter baumannii
NADPH
-
Acinetobacter baumannii
[2Fe-2S]-center
-
Acinetobacter baumannii
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
E205A
inactive
Acinetobacter baumannii
E205D
inactive
Acinetobacter baumannii
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-carnitine + NADH + H+ + O2
Acinetobacter baumannii
-
(3R)-3-hydroxy-4-oxobutanoate + trimethylamine + NAD+ + H2O
-
-
?
L-carnitine + NADH + H+ + O2
Acinetobacter baumannii ATCC19606
-
(3R)-3-hydroxy-4-oxobutanoate + trimethylamine + NAD+ + H2O
-
-
?
L-carnitine + NADPH + H+ + O2
Acinetobacter baumannii
-
(3R)-3-hydroxy-4-oxobutanoate + trimethylamine + NADP+ + H2O
-
-
?
L-carnitine + NADPH + H+ + O2
Acinetobacter baumannii ATCC19606
-
(3R)-3-hydroxy-4-oxobutanoate + trimethylamine + NADP+ + H2O
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
His-tag affinity column chromatography
Acinetobacter baumannii
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-carnitine + NADH + H+ + O2
-
746297
Acinetobacter baumannii
(3R)-3-hydroxy-4-oxobutanoate + trimethylamine + NAD+ + H2O
-
-
-
?
L-carnitine + NADH + H+ + O2
-
746297
Acinetobacter baumannii ATCC19606
(3R)-3-hydroxy-4-oxobutanoate + trimethylamine + NAD+ + H2O
-
-
-
?
L-carnitine + NADPH + H+ + O2
-
746297
Acinetobacter baumannii
(3R)-3-hydroxy-4-oxobutanoate + trimethylamine + NADP+ + H2O
-
-
-
?
L-carnitine + NADPH + H+ + O2
-
746297
Acinetobacter baumannii ATCC19606
(3R)-3-hydroxy-4-oxobutanoate + trimethylamine + NADP+ + H2O
-
-
-
?
Other publictions for EC 1.14.13.239
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
744652
Koeth
gamma-Butyrobetaine is a proa ...
Escherichia coli, Escherichia coli DH10B
Cell Metab.
20
799-812
2014
-
-
1
-
-
-
-
-
-
-
-
4
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
746297
Zhu
Carnitine metabolism to trime ...
Acinetobacter baumannii, Acinetobacter baumannii ATCC19606
Proc. Natl. Acad. Sci. USA
111
4268-4273
2014
-
-
1
-
2
-
-
-
-
-
-
4
-
7
-
-
1
-
-
-
-
-
4
-
-
-
-
-
-
-
-
5
-
-
-
-
-
1
5
-
2
-
-
-
-
-
-
-
-
4
-
-
-
1
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
744480
Ditullio
L-carnitine via enzyme-cataly ...
Acinetobacter calcoaceticus, Acinetobacter calcoaceticus ATCC 39647, Acinetobacter calcoaceticus ATCC 39648
Bioorg. Med. Chem.
2
415-420
1994
-
-
-
-
-
-
1
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
12
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
12
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-