Crystallization (Comment) | Organism |
---|---|
in complex with tetracyclines minocycline and tigecycline at 2.18 and 2.30 A resolution, respectively. Both tetracyclines bind in a large tunnel-shaped active site in close contact to the cofactor FAD, preoriented for regioselective hydroxylation to 11alpha-hydroxytetracyclines. The bulky 9-tert-butylglycylamido substituent of tigecycline is solvent-exposed and does not interfere with TetX binding. In the TetX-minocycline complex a second binding site for a minocycline dimer is observed close to the active-site entrance. The putative dioxygen-binding cavities are located in the substrate-binding domain next to the active site | Bacteroides thetaiotaomicron |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacteroides thetaiotaomicron | Q93L51 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
minocycline + NADPH + H+ + O2 | - |
Bacteroides thetaiotaomicron | 11a-hydroxyminocycline + NADP+ + H2O | - |
? | |
tigecycline + NADPH + H+ + O2 | - |
Bacteroides thetaiotaomicron | 11a-hydroxytigecycline + NADP+ + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
TetX family tetracycline inactivation enzyme | - |
Bacteroides thetaiotaomicron |
TetX2 | - |
Bacteroides thetaiotaomicron |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Bacteroides thetaiotaomicron |