Literature summary for 1.14.13.230 extracted from

  • Dubbels, B.L.; Sayavedra-Soto, L.A.; Arp, D.J.
    Butane monooxygenase of Pseudomonas butanovora: purification and biochemical characterization of a terminal-alkane hydroxylating diiron monooxygenase (2007), Microbiology, 153, 1808-1816.
    View publication on PubMed

Organism

Organism UniProt Comment Textmining
Thauera butanivorans Q8KQF0 and Q8KQE9 and Q8KQE7 and Q8KQE6 and Q8KQE8 Q8KQF0 i.e. alpha-subunit BmoX, Q8KQE9 i.e. beta-subunit BmoY, Q8KQE7 i.e. gamma-subunit BmoZ of hydrolase component, respectively. Q8KQE6 i.e. oxidoreductase BmoC, Q8KQE8 i.e. regulatory protein BmoB
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Thauera butanivorans ATCC 43655 Q8KQF0 and Q8KQE9 and Q8KQE7 and Q8KQE6 and Q8KQE8 Q8KQF0 i.e. alpha-subunit BmoX, Q8KQE9 i.e. beta-subunit BmoY, Q8KQE7 i.e. gamma-subunit BmoZ of hydrolase component, respectively. Q8KQE6 i.e. oxidoreductase BmoC, Q8KQE8 i.e. regulatory protein BmoB
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the reconstituted enzyme complex oxidizes C3-C6 linear and branched aliphatic alkanes. Regiospecificity is strongly biased towards primary hydroxylation, with 80% of the hydroxylations occurring at the terminal carbon atom. The hydrolase component BMOH is capable of ethene oxidation when supplied with H2O2 and ethene Thauera butanivorans ?
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additional information the reconstituted enzyme complex oxidizes C3-C6 linear and branched aliphatic alkanes. Regiospecificity is strongly biased towards primary hydroxylation, with 80% of the hydroxylations occurring at the terminal carbon atom. The hydrolase component BMOH is capable of ethene oxidation when supplied with H2O2 and ethene Thauera butanivorans ATCC 43655 ?
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?
butane + NADH + H+ + O2
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Thauera butanivorans butan-1-ol + NAD+ + H2O about 83% butan-1-ol plus 17% butan-2-ol, in presence and absence of regulatory subunit BmoB ?
butane + NADH + H+ + O2
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Thauera butanivorans ATCC 43655 butan-1-ol + NAD+ + H2O about 83% butan-1-ol plus 17% butan-2-ol, in presence and absence of regulatory subunit BmoB ?
hexane + NADH + H+ + O2
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Thauera butanivorans hexan-1-ol + NAD+ + H2O about 90% hexan-1-ol plus 10% hexan-2-ol in absence, and 86% hexan-1-ol plus 14% hexan-2-ol in presence of regulatory subunit BmoB, respectively ?
hexane + NADH + H+ + O2
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Thauera butanivorans ATCC 43655 hexan-1-ol + NAD+ + H2O about 90% hexan-1-ol plus 10% hexan-2-ol in absence, and 86% hexan-1-ol plus 14% hexan-2-ol in presence of regulatory subunit BmoB, respectively ?
isobutane + NADH + H+ + O2
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Thauera butanivorans 2-methyl-1-propanol + NAD+ + H2O about 95% 2-methyl-1-propanol plus 5% 2-methyl-2-propanol in absence, and 96% 2-methyl-1-propanol plus 4% 2-methyl-2-propanol in presence of regulatory subunit BmoB, respectively ?
isobutane + NADH + H+ + O2
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Thauera butanivorans ATCC 43655 2-methyl-1-propanol + NAD+ + H2O about 95% 2-methyl-1-propanol plus 5% 2-methyl-2-propanol in absence, and 96% 2-methyl-1-propanol plus 4% 2-methyl-2-propanol in presence of regulatory subunit BmoB, respectively ?
isopentane + NADH + H+ + O2
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Thauera butanivorans 3-methyl-1-butanol + 2-methyl-1-butanol + NAD+ + H2O about 63% 3-methyl-1-butanol plus 31% 2-methyl-1-butanol + 2% 3-methyl-2-butanol + 4% 2-methyl-2-butanol in absence, and 64% 3-methyl-1-butanol plus 31% 2-methyl-1-butanol + 1% 3-methyl-2-butanol + 4% 2-methyl-2-butanol in presence of regulatory subunit BmoB, respectively ?
isopentane + NADH + H+ + O2
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Thauera butanivorans ATCC 43655 3-methyl-1-butanol + 2-methyl-1-butanol + NAD+ + H2O about 63% 3-methyl-1-butanol plus 31% 2-methyl-1-butanol + 2% 3-methyl-2-butanol + 4% 2-methyl-2-butanol in absence, and 64% 3-methyl-1-butanol plus 31% 2-methyl-1-butanol + 1% 3-methyl-2-butanol + 4% 2-methyl-2-butanol in presence of regulatory subunit BmoB, respectively ?
pentane + NADH + H+ + O2
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Thauera butanivorans pentan-1-ol + NAD+ + H2O about 80% pentan-1-ol plus 13% pentan-2-ol and 7% pentan-3-ol in absence, and 88% pentan-1-ol plus 11% pentan-2-ol and 1% pentan-3-ol in presence of regulatory subunit BmoB, respectively ?
propane + NADH + H+ + O2
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Thauera butanivorans propan-1-ol + NAD+ + H2O about 82% propan-1-ol plus 18% propan-2-ol, in presence and absence of regulatory subunit BmoB ?