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Literature summary for 1.14.13.23 extracted from

  • Chen, R.; Oki, H.; Chaen, H.; Hosokawa, K.
    Studies on m-hydroxybenzoate 4-hydroxylase from Comamonas testosteroni I. Purification and characterization (1997), Res. Commun. Biochem. Cell Mol. Biol., 1, 304-322.
No PubMed abstract available
Search for more literature for 1.14.13.23

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03
-
 NADPH
-
 Comamonas testosteroni
0.03
-
 m-Hydroxybenzoate
-
 Comamonas testosteroni

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
71000
-
 x * 71000, SDS-PAGE Comamonas testosteroni

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-hydroxybenzoate + [reduced NADPH-hemoprotein reductase] + O2 Comamonas testosteroni
-
 3,4-dihydroxybenzoate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Comamonas testosteroni
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Comamonas testosteroni

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.17
-
-
 Comamonas testosteroni

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,3-dihydroxybenzoate + NADPH + O2
-
 Comamonas testosteroni ?
-
 ?
3-hydroxybenzoate + [reduced NADPH-hemoprotein reductase] + O2
-
 Comamonas testosteroni 3,4-dihydroxybenzoate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
gentisate + NADPH + O2
-
 Comamonas testosteroni ?
-
 ?

Subunits

Subunits Comment Organism
? x * 71000, SDS-PAGE Comamonas testosteroni

Cofactor

Cofactor Comment Organism Structure
FAD flavoprotein Comamonas testosteroni
NADPH specific requirement for NADPH Comamonas testosteroni