Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Chromobacterium violaceum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
48000 | - |
x * 48000, SDS-PAGE | Chromobacterium violaceum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chromobacterium violaceum | Q9S3U9 | - |
- |
Chromobacterium violaceum DSM 30191 | Q9S3U9 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
prodeoxyviolacein + NAD(P)H + O2 | - |
Chromobacterium violaceum | deoxyviolacein + NAD(P)+ + H2O | - |
? | |
prodeoxyviolacein + NAD(P)H + O2 | - |
Chromobacterium violaceum DSM 30191 | deoxyviolacein + NAD(P)+ + H2O | - |
? | |
proviolacein + NAD(P)H + O2 | VioC is able to hydroxylate proviolacein | Chromobacterium violaceum | violacein + NAD(P)+ + H2O | - |
? | |
proviolacein + NAD(P)H + O2 | VioC is able to hydroxylate proviolacein | Chromobacterium violaceum DSM 30191 | violacein + NAD(P)+ + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 48000, SDS-PAGE | Chromobacterium violaceum |
Synonyms | Comment | Organism |
---|---|---|
VioC | - |
Chromobacterium violaceum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Chromobacterium violaceum | |
NADH | - |
Chromobacterium violaceum | |
NADPH | - |
Chromobacterium violaceum |
General Information | Comment | Organism |
---|---|---|
physiological function | flavin-dependent oxygenases, VioC and VioD, act sequentially in biosynthesis of violacein. VioD hydroxylates one indole ring at the 5-position to yield proviolacein, and VioC then acts on the other indole ring at the 2-position to create the oxindole and complete violacein formation. Deletion of both VioC and VioD leads to formation of a green pigment, deoxychromoviridans, in Top10 cells. When this same construct is transformed into BL21 cells, purple pigment is produced, containing deoxychromoviridans, prodeoxyviolacein, and various other two- and four-electron oxidized versions of prodeoxyviolacein | Chromobacterium violaceum |