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Literature summary for 1.14.13.224 extracted from

  • Balibar, C.J.; Walsh, C.T.
    In vitro biosynthesis of violacein from L-tryptophan by the enzymes VioA-E from Chromobacterium violaceum (2006), Biochemistry, 45, 15444-15457.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Chromobacterium violaceum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48000
-
x * 48000, SDS-PAGE Chromobacterium violaceum

Organism

Organism UniProt Comment Textmining
Chromobacterium violaceum Q9S3U9
-
-
Chromobacterium violaceum DSM 30191 Q9S3U9
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
prodeoxyviolacein + NAD(P)H + O2
-
Chromobacterium violaceum deoxyviolacein + NAD(P)+ + H2O
-
?
prodeoxyviolacein + NAD(P)H + O2
-
Chromobacterium violaceum DSM 30191 deoxyviolacein + NAD(P)+ + H2O
-
?
proviolacein + NAD(P)H + O2 VioC is able to hydroxylate proviolacein Chromobacterium violaceum violacein + NAD(P)+ + H2O
-
?
proviolacein + NAD(P)H + O2 VioC is able to hydroxylate proviolacein Chromobacterium violaceum DSM 30191 violacein + NAD(P)+ + H2O
-
?

Subunits

Subunits Comment Organism
? x * 48000, SDS-PAGE Chromobacterium violaceum

Synonyms

Synonyms Comment Organism
VioC
-
Chromobacterium violaceum

Cofactor

Cofactor Comment Organism Structure
FAD
-
Chromobacterium violaceum
NADH
-
Chromobacterium violaceum
NADPH
-
Chromobacterium violaceum

General Information

General Information Comment Organism
physiological function flavin-dependent oxygenases, VioC and VioD, act sequentially in biosynthesis of violacein. VioD hydroxylates one indole ring at the 5-position to yield proviolacein, and VioC then acts on the other indole ring at the 2-position to create the oxindole and complete violacein formation. Deletion of both VioC and VioD leads to formation of a green pigment, deoxychromoviridans, in Top10 cells. When this same construct is transformed into BL21 cells, purple pigment is produced, containing deoxychromoviridans, prodeoxyviolacein, and various other two- and four-electron oxidized versions of prodeoxyviolacein Chromobacterium violaceum