Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Stigmatella aurantiaca |
expression in Escherichia coli | Stigmatella aurantiaca |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
41290 | - |
calculated from amino acid sequence | Stigmatella aurantiaca |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
aurachin C + NADH + O2 | Stigmatella aurantiaca | - |
4-hydroxy-2-methyl-3-oxo-4-farnesyl-3,4-dihydroquinoline-1-oxide + NAD+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Stigmatella aurantiaca | H1ZZA4 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
aurachin C + NAD(P)H + H+ + O2 | - |
Stigmatella aurantiaca | 4-hydroxy-2-methyl-3-oxo-4-[(2E,6E)-farnesyl]-3,4-dihydroquinoline 1-oxide + NAD(P)+ + H2O | overall reaction, the product of AuaG is highly unstable and rapidly isomerizes in 0.1 M CHES (pH 9.5)/methanol | ? | |
aurachin C + NADH + H+ + O2 | - |
Stigmatella aurantiaca | 4-hydroxy-2-methyl-4-[(2E,6E)-3,7,11-trimethyldodeca-2,6,10-trien-1-yl]quinolin-3(4H)-one 1-oxide + NAD+ + H2O | formation of aurachin B requires the presence of AuaH. AuaG and AuaH act sequentially and conversion of aurachin C into aurachin B occurs by oxidation and subsequent reduction | ? | |
aurachin C + NADH + O2 | - |
Stigmatella aurantiaca | 4-hydroxy-2-methyl-3-oxo-4-farnesyl-3,4-dihydroquinoline-1-oxide + NAD+ + H2O | - |
? | |
aurachin C + NADH + O2 | first step in the conversion of aurachin C to aurachin. The FAD-dependent monooxygenase catalyses the epoxidation of the C2-C3 double bond of aurachin C, this is followed by a semipinacol rearrangement, causing migration of the farnesyl group from C3 to C4 | Stigmatella aurantiaca | 4-hydroxy-2-methyl-3-oxo-4-farnesyl-3,4-dihydroquinoline-1-oxide + NAD+ + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
auaG | - |
Stigmatella aurantiaca |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | contains an FAD binding domain | Stigmatella aurantiaca | |
FAD | AuaG purifies as a slightly yellow protein and the binding cofactor is flavinadenine dinucleotide | Stigmatella aurantiaca | |
NADH | NADH is preferred over NADPH | Stigmatella aurantiaca | |
NADH | preferred over NADPH | Stigmatella aurantiaca | |
NADPH | NADH is preferred over NADPH | Stigmatella aurantiaca |