Literature summary for 1.14.13.22 extracted from

Li, Y.; Zheng, Y.; Geng, Q.; Liu, F.; Zhang, Z.; Xu, J.; Yu, H.
Secretory expression of cyclohexanone monooxygenase by methylotrophic yeast for efficient omeprazole sulfide bio-oxidation (2021), Bioresour. Bioprocess., 8, 81 .

No PubMed abstract available

Search for more literature for 1.14.13.22

Application

Application	Comment	Organism
synthesis	extracellular production of engineered CHMO by Pichia pastoris. The recombinant CHMO shows a higher flavin occupation rate than that produced by Escherichia coli, accompanied by a 3.2fold increase in catalytic efficiency. At a cell density of 150 g/l cell dry weight, a recombinant CHMO production rate of 1,700 U/l is achieved. By directly employing the pH adjusted supernatant as a biocatalyst, 10 g/l of pyrmetazole is almost completely transformed into the corresponding (S)-sulfoxide, with > 99% enantiomeric excess	Acinetobacter sp.

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.00071	-	NADPH	recombinant engineered enzyme expressed in Pichia pastoris, pH 8.0, temperature not specified in the publication	Acinetobacter sp.
0.0011	-	NADPH	recombinant engineered enzyme expressed in Escherichia coli, pH 8.0, temperature not specified in the publication	Acinetobacter sp.
0.026	-	pyrmetazole	recombinant engineered enzyme expressed in Pichia pastoris, pH 8.0, temperature not specified in the publication	Acinetobacter sp.
0.028	-	pyrmetazole	recombinant engineered enzyme expressed in Escherichia coli, pH 8.0, temperature not specified in the publication	Acinetobacter sp.

Organism

Organism	UniProt	Comment	Textmining
Acinetobacter sp.	P12015	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
pyrmetazole + NADPH + H+ + O2	-	Acinetobacter sp.	pyrmetazole (S)-sulfoxide + NADP+ + H2O	-	?

Synonyms

Synonyms	Comment	Organism
CHMO	-	Acinetobacter sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.34	-	NADPH	recombinant engineered enzyme expressed in Escherichia coli, pH 8.0, temperature not specified in the publication	Acinetobacter sp.
0.35	-	pyrmetazole	recombinant engineered enzyme expressed in Escherichia coli, pH 8.0, temperature not specified in the publication	Acinetobacter sp.
0.82	-	NADPH	recombinant engineered enzyme expressed in Pichia pastoris, pH 8.0, temperature not specified in the publication	Acinetobacter sp.
1.12	-	pyrmetazole	recombinant engineered enzyme expressed in Pichia pastoris, pH 8.0, temperature not specified in the publication	Acinetobacter sp.

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
12.5	-	pyrmetazole	recombinant engineered enzyme expressed in Escherichia coli, pH 8.0, temperature not specified in the publication	Acinetobacter sp.
43.4	-	pyrmetazole	recombinant engineered enzyme expressed in Pichia pastoris, pH 8.0, temperature not specified in the publication	Acinetobacter sp.
304	-	NADPH	recombinant engineered enzyme expressed in Escherichia coli, pH 8.0, temperature not specified in the publication	Acinetobacter sp.
1154	-	NADPH	recombinant engineered enzyme expressed in Pichia pastoris, pH 8.0, temperature not specified in the publication	Acinetobacter sp.

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Release 2023.1 (January 2023)