synthesis |
synthesis od lactones from cycloalkanes. A heterologous pathway comprising enzymes with compatible kinetics is designed in Pseudomonas taiwanensis VLB120 enabling in-vivo cascade for synthesizing lactones from cycloalkanes. The respective pathway includes cytochrome P450 monooxygenase (CHX), cyclohexanol dehydrogenase (CDH), and cyclohexanone monooxygenase (CHXON) from Acidovorax sp. CHX100. Resting cells of the recombinant host Pseudomonas taiwanensis VLB120 convert cyclohexane, cyclohexanol, and cyclohexanone to epsilon-caprolactone at 22, 80-100, and 170 U/g cell dry weight, respectively. Cyclohexane (5 mM) is completely converted with a selectivity of 65% for epsilon-caprolactone formation in 2 h without accumulation of intermediate products |
Acidovorax sp. CHX100 |
synthesis |
synthesis of lactones from cycloalkanes in Pseudomonas taiwanensis VLB120. The pathway includes cytochrome P450 monooxygenase, cyclohexanol dehydrogenase, and cyclohexanone monooxygenase from Acidovorax sp. CHX100. Recombinant P. taiwanensis converts cyclohexane, cyclohexanol, and cyclohexanone to epsilon-caprolactone at 22, 80-100, and 170 U/gCDW, respectively. Cyclohexane (5 mM) is completely converted with a selectivity of 65% for epsilon-caprolactone formation in 2 hr without accumulation of intermediate products. Analogous lactones can be obtained from cyclooctane and cyclodecane |
Acidovorax sp. CHX100 |