Crystallization (Comment) | Organism |
---|---|
- |
Thermocrispum municipale |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.009 | - |
cyclohexanone | pH 7.0, 25°C | Acinetobacter calcoaceticus | |
0.02 | - |
Cyclopentanone | pH 7.0, 25°C | Thermocrispum municipale | |
0.2 | - |
phenylacetone | pH 7.0, 25°C | Thermocrispum municipale | |
0.4 | - |
Cyclopentanone | pH 7.0, 25°C | Acinetobacter calcoaceticus | |
0.5 | - |
2-butanone | pH 7.0, 25°C | Thermocrispum municipale | |
1 | - |
thioanisole | pH 7.0, 25°C | Acinetobacter calcoaceticus | |
3 | - |
phenylacetone | pH 7.0, 25°C | Acinetobacter calcoaceticus | |
5 | - |
acetophenone | pH 7.0, 25°C | Thermocrispum municipale | |
13 | - |
acetophenone | pH 7.0, 25°C | Acinetobacter calcoaceticus |
Organic Solvent | Comment | Organism |
---|---|---|
acetonitrile | 25 min incubation in 14% acetonitrile at 20°C, enzyme retain more than 80% of its activity | Thermocrispum municipale |
acetonitrile | 25 min incubation in 14% acetonitrile at 20°C, loss of activity | Acinetobacter calcoaceticus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acinetobacter calcoaceticus | - |
- |
- |
Acinetobacter calcoaceticus NCIMB 9871 | - |
- |
- |
Thermocrispum municipale | A0A1L1QK39 | - |
- |
Thermocrispum municipale DSM 44D49 | A0A1L1QK39 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Thermocrispum municipale |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 thioanisole + NADPH + H+ + O2 | the enzyme produces both enantiomers, leading to an ee of only 16% for the (R)-sulfoxide | Thermocrispum municipale | (R)-thioanisole sulfoxide + (S)-thioanisole sulfoxide + 2 NADP+ + 2 H2O | - |
? | |
2 thioanisole + NADPH + H+ + O2 | the enzyme produces both enantiomers, leading to an ee of only 16% for the (R)-sulfoxide | Thermocrispum municipale DSM 44D49 | (R)-thioanisole sulfoxide + (S)-thioanisole sulfoxide + 2 NADP+ + 2 H2O | - |
? | |
2-butanone + NADPH + H+ + O2 | the ratio between the products methyl propanoate and ethyl acetate was found to be about 3:7 in the absence of any cosolvent | Acinetobacter calcoaceticus | ethyl acetate + NADP+ + H2O | - |
? | |
2-butanone + NADPH + H+ + O2 | the ratio between the products methyl propanoate and ethyl acetate was found to be about 3:7 in the absence of any cosolvent. The effect of various solvents at 15% concentration is inspected. The strongest effect on regioselectivity is observed with 2-methyl-1,3-dioxolane (15%), which leads to almost exclusive production of ethyl acetate. 1,3-Dioxane and 1,4-dioxane have a more moderate influence, since about 40% of the total product is methyl propanoate. 30% methanol or ethanol as cosolvents have a negligible effect on enzyme regioselectivity | Thermocrispum municipale | ethyl acetate + NADP+ + H2O | - |
? | |
2-butanone + NADPH + H+ + O2 | the ratio between the products methyl propanoate and ethyl acetate was found to be about 3:7 in the absence of any cosolvent | Acinetobacter calcoaceticus NCIMB 9871 | ethyl acetate + NADP+ + H2O | - |
? | |
2-butanone + NADPH + H+ + O2 | the ratio between the products methyl propanoate and ethyl acetate was found to be about 3:7 in the absence of any cosolvent. The effect of various solvents at 15% concentration is inspected. The strongest effect on regioselectivity is observed with 2-methyl-1,3-dioxolane (15%), which leads to almost exclusive production of ethyl acetate. 1,3-Dioxane and 1,4-dioxane have a more moderate influence, since about 40% of the total product is methyl propanoate. 30% methanol or ethanol as cosolvents have a negligible effect on enzyme regioselectivity | Thermocrispum municipale DSM 44D49 | ethyl acetate + NADP+ + H2O | - |
? | |
2-butanone + NADPH + H+ + O2 | the ratio between the products methyl propanoate and ethyl acetate was found to be about 3:7 in the absence of any cosolvent | Acinetobacter calcoaceticus | methyl propanoate + NADP+ + H2O | - |
? | |
2-butanone + NADPH + H+ + O2 | the ratio between the products methyl propanoate and ethyl acetate was found to be about 3:7 in the absence of any cosolvent. The effect of various solvents at 15% concentration is inspected. The strongest effect on regioselectivity is observed with 2-methyl-1,3-dioxolane (15%), which leads to almost exclusive production of ethyl acetate. 1,3-Dioxane and 1,4-dioxane have a more moderate influence, since about 40% of the total product is methyl propanoate. 30% methanol or ethanol as cosolvents have a negligible effect on enzyme regioselectivity | Thermocrispum municipale | methyl propanoate + NADP+ + H2O | - |
? | |
acetophenone + NADPH + H+ + O2 | - |
Acinetobacter calcoaceticus | ? | - |
? | |
acetophenone + NADPH + H+ + O2 | - |
Thermocrispum municipale | ? | - |
? | |
bicyclo[3.2.0]hept-2-en-6-one + NADPH + H+ + O2 | - |
Acinetobacter calcoaceticus | ? | - |
? | |
bicyclo[3.2.0]hept-2-en-6-one + NADPH + H+ + O2 | the enzyme produces all four possible lactones | Thermocrispum municipale | ? | - |
? | |
bicyclo[3.2.0]hept-2-en-6-one + NADPH + H+ + O2 | - |
Acinetobacter calcoaceticus NCIMB 9871 | ? | - |
? | |
bicyclo[3.2.0]hept-2-en-6-one + NADPH + H+ + O2 | the enzyme produces all four possible lactones | Thermocrispum municipale DSM 44D49 | ? | - |
? | |
cyclobutanone + NADPH + H+ + O2 | - |
Acinetobacter calcoaceticus | ? | - |
? | |
cyclobutanone + NADPH + H+ + O2 | - |
Thermocrispum municipale | ? | - |
? | |
cyclohexanone + NADPH + H+ + O2 | - |
Acinetobacter calcoaceticus | hexano-6-lactone + NADP+ + H2O | - |
? | |
cyclohexanone + NADPH + H+ + O2 | - |
Thermocrispum municipale | hexano-6-lactone + NADP+ + H2O | - |
? | |
cyclohexanone + NADPH + H+ + O2 | - |
Acinetobacter calcoaceticus NCIMB 9871 | hexano-6-lactone + NADP+ + H2O | - |
? | |
cyclohexanone + NADPH + H+ + O2 | - |
Thermocrispum municipale DSM 44D49 | hexano-6-lactone + NADP+ + H2O | - |
? | |
cyclopentanone + NADPH + H+ + O2 | - |
Acinetobacter calcoaceticus | ? | - |
? | |
cyclopentanone + NADPH + H+ + O2 | - |
Thermocrispum municipale | ? | - |
? | |
cyclopentanone + NADPH + H+ + O2 | - |
Acinetobacter calcoaceticus NCIMB 9871 | ? | - |
? | |
cyclopentanone + NADPH + H+ + O2 | - |
Thermocrispum municipale DSM 44D49 | ? | - |
? | |
phenylacetone + NADPH + H+ + O2 | - |
Acinetobacter calcoaceticus | ? | - |
? | |
phenylacetone + NADPH + H+ + O2 | - |
Thermocrispum municipale | ? | - |
? | |
thioanisole + NADPH + H+ + O2 | - |
Acinetobacter calcoaceticus | ? | - |
? | |
thioanisole + NADPH + H+ + O2 | - |
Acinetobacter calcoaceticus NCIMB 9871 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CHMO | - |
Acinetobacter calcoaceticus |
CHMO | - |
Thermocrispum municipale |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
Tm-value | Acinetobacter calcoaceticus |
45 | - |
the enzyme displays 58% residual activity after 5.5 h at 45°C | Thermocrispum municipale |
48 | - |
Tm-value | Thermocrispum municipale |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
acetophenone | pH 7.0, 25°C | Thermocrispum municipale | |
0.3 | - |
2-butanone | pH 7.0, 25°C | Acinetobacter calcoaceticus | |
0.4 | - |
acetophenone | pH 7.0, 25°C | Acinetobacter calcoaceticus | |
0.4 | - |
2-butanone | pH 7.0, 25°C | Thermocrispum municipale | |
1 | - |
Cyclopentanone | pH 7.0, 25°C | Acinetobacter calcoaceticus | |
1 | - |
phenylacetone | pH 7.0, 25°C | Acinetobacter calcoaceticus | |
1 | - |
phenylacetone | pH 7.0, 25°C | Thermocrispum municipale | |
2 | - |
cyclohexanone | pH 7.0, 25°C | Thermocrispum municipale | |
2 | - |
Cyclopentanone | pH 7.0, 25°C | Thermocrispum municipale | |
2 | - |
thioanisole | pH 7.0, 25°C | Thermocrispum municipale | |
2 | - |
Cyclobutanone | pH 7.0, 25°C | Thermocrispum municipale | |
2 | - |
bicyclo[3.2.0]hept-2-en-6-one | pH 7.0, 25°C | Thermocrispum municipale | |
3 | - |
thioanisole | pH 7.0, 25°C | Acinetobacter calcoaceticus | |
4 | - |
Cyclobutanone | pH 7.0, 25°C | Acinetobacter calcoaceticus | |
4 | - |
bicyclo[3.2.0]hept-2-en-6-one | pH 7.0, 25°C | Acinetobacter calcoaceticus | |
6 | - |
cyclohexanone | pH 7.0, 25°C | Acinetobacter calcoaceticus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
acetophenone | pH 7.0, 25°C | Thermocrispum municipale | |
0.03 | - |
acetophenone | pH 7.0, 25°C | Acinetobacter calcoaceticus | |
0.3 | - |
phenylacetone | pH 7.0, 25°C | Acinetobacter calcoaceticus | |
1 | - |
2-butanone | pH 7.0, 25°C | Thermocrispum municipale | |
3 | - |
Cyclopentanone | pH 7.0, 25°C | Acinetobacter calcoaceticus | |
3 | - |
thioanisole | pH 7.0, 25°C | Acinetobacter calcoaceticus | |
5 | - |
phenylacetone | pH 7.0, 25°C | Thermocrispum municipale | |
100 | - |
Cyclopentanone | pH 7.0, 25°C | Thermocrispum municipale | |
667 | - |
cyclohexanone | pH 7.0, 25°C | Acinetobacter calcoaceticus |