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Literature summary for 1.14.13.22 extracted from

  • Leipold, F.; Wardenga, R.; Bornscheuer, U.T.
    Cloning, expression and characterization of a eukaryotic cycloalkanone monooxygenase from Cylindrocarpon radicicola ATCC 11011 (2012), Appl. Microbiol. Biotechnol., 94, 705-717.
    View publication on PubMed
Search for more literature for 1.14.13.22

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Ilyonectria destructans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.007
-
 Cyclobutanone pH 8.0, 25°C Ilyonectria destructans
0.04
-
 cyclohexanone pH 8.0, 25°C Ilyonectria destructans
0.12
-
 2-hydroxycyclobutanone pH 8.0, 25°C Ilyonectria destructans
0.33
-
 2-phenylcyclohexanone pH 8.0, 25°C Ilyonectria destructans
0.88
-
 Cycloheptanone pH 8.0, 25°C Ilyonectria destructans
1.03
-
 Cyclooctanone pH 8.0, 25°C Ilyonectria destructans
4.66
-
 Cyclopentanone pH 8.0, 25°C Ilyonectria destructans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54000
-
 1 * 61900, calculated, 1 * 54000, SDS-PAGE Ilyonectria destructans
61900
-
 1 * 61900, calculated, 1 * 54000, SDS-PAGE Ilyonectria destructans
76000
-
 gel filtration Ilyonectria destructans

Organism

Organism UniProt Comment Textmining
Ilyonectria destructans G8H1L8
-
-
Ilyonectria destructans ATCC 11011 G8H1L8
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.8
-
 substrate cyclohexanone, pH 8.0, 25°C Ilyonectria destructans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(1R)-bicyclo[2.2.1]heptane-2,5-dione + NADPH + O2 74.4% of the activity with cyclohexanone Ilyonectria destructans ? + NADP+ + H2O
-
 ?
2-hydroxycyclobutanone + NADPH + H+ + O2 67.3% of the activity with cyclohexanone Ilyonectria destructans 1-oxa-2-oxo-3-hydroxycyclopentane + NADP+ + H2O
-
 ?
2-methyl-cyclohexanone + NADPH + H+ + O2 79.2% of the activity with cyclohexanone Ilyonectria destructans 1-oxa-2-oxo-3-methylcycloheptane + NADP+ + H2O
-
 ?
2-methyl-cyclohexanone + NADPH + H+ + O2 79.2% of the activity with cyclohexanone Ilyonectria destructans ATCC 11011 1-oxa-2-oxo-3-methylcycloheptane + NADP+ + H2O
-
 ?
2-methyl-cyclopentanone + NADPH + H+ + O2 60.5% of the activity with cyclohexanone Ilyonectria destructans 1-oxa-2-oxo-3-methylcyclohexane + NADP+ + H2O
-
 ?
2-methyl-cyclopentanone + NADPH + H+ + O2 60.5% of the activity with cyclohexanone Ilyonectria destructans ATCC 11011 1-oxa-2-oxo-3-methylcyclohexane + NADP+ + H2O
-
 ?
2-phenylcyclohexanone + NADPH + H+ + O2 11% of the activity with cyclohexanone Ilyonectria destructans 1-oxa-2-oxo-3-phenylcycloheptane + NADP+ + H2O
-
 ?
bicyclo[3.2.0]hept-2-en-6-one + NADPH + H+ + O2 63% of the activity with cyclohexanone Ilyonectria destructans 3,3a,6,6a-tetrahydro-1H-cyclopenta[c]furan-1-one + NADP+ + H2O
-
 ?
cyclobutanone + NADPH + H+ + O2 86.9% of the activity with cyclohexanone Ilyonectria destructans 1-oxa-2-oxo-cyclopentane + NADP+ + H2O
-
 ?
cyclobutanone + NADPH + H+ + O2 86.9% of the activity with cyclohexanone Ilyonectria destructans ATCC 11011 1-oxa-2-oxo-cyclopentane + NADP+ + H2O
-
 ?
cyclobutyl methyl ketone + NADPH + O2 17.5% of the activity with cyclohexanone Ilyonectria destructans ? + NADP+ + H2O
-
 ?
cycloheptanone + NADPH + H+ + O2 23.5% of the activity with cyclohexanone Ilyonectria destructans 1-oxa-2-oxo-cyclooctane + NADP+ + H2O
-
 ?
cycloheptanone + NADPH + H+ + O2 23.5% of the activity with cyclohexanone Ilyonectria destructans ATCC 11011 1-oxa-2-oxo-cyclooctane + NADP+ + H2O
-
 ?
cyclohexanone + NADPH + H+ + O2 best substrate Ilyonectria destructans epsilon-caprolactone + NADP+ + H2O
-
 ?
cyclohexanone + NADPH + H+ + O2 best substrate Ilyonectria destructans ATCC 11011 epsilon-caprolactone + NADP+ + H2O
-
 ?
cyclohexyl methyl ketone + NADPH + O2 15.7% of the activity with cyclohexanone Ilyonectria destructans ? + NADP+ + H2O
-
 ?
cyclooctanone + NADPH + H+ + O2 3.1% of the activity with cyclohexanone Ilyonectria destructans 1-oxa-2-oxo-cyclononane + NADP+ + H2O
-
 ?
cyclopentanone + NADPH + H+ + O2 11.3% of the activity with cyclohexanone Ilyonectria destructans 1-oxa-2-oxo-cyclohexane + NADP+ + H2O
-
 ?
norcamphor + NADPH + O2 74.8% of the activity with cyclohexanone Ilyonectria destructans ? + NADP+ + H2O
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 61900, calculated, 1 * 54000, SDS-PAGE Ilyonectria destructans

Synonyms

Synonyms Comment Organism
CAMO
-
 Ilyonectria destructans
cycloalkaone monooxygenase
-
 Ilyonectria destructans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.68
-
 Cyclooctanone pH 8.0, 25°C Ilyonectria destructans
0.73
-
 2-phenylcyclohexanone pH 8.0, 25°C Ilyonectria destructans
4.3
-
 Cycloheptanone pH 8.0, 25°C Ilyonectria destructans
4.5
-
 2-hydroxycyclobutanone pH 8.0, 25°C Ilyonectria destructans
5.9
-
 Cyclopentanone pH 8.0, 25°C Ilyonectria destructans
6.8
-
 Cyclobutanone pH 8.0, 25°C Ilyonectria destructans
9.8
-
 cyclohexanone pH 8.0, 25°C Ilyonectria destructans

Cofactor

Cofactor Comment Organism Structure
additional information no cofactor: NADH Ilyonectria destructans
NADPH
-
 Ilyonectria destructans

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.7
-
 Cyclooctanone pH 8.0, 25°C Ilyonectria destructans
1.3
-
 Cyclopentanone pH 8.0, 25°C Ilyonectria destructans
2.3
-
 2-phenylcyclohexanone pH 8.0, 25°C Ilyonectria destructans
4.9
-
 Cycloheptanone pH 8.0, 25°C Ilyonectria destructans
38
-
 2-hydroxycyclobutanone pH 8.0, 25°C Ilyonectria destructans
238
-
 cyclohexanone pH 8.0, 25°C Ilyonectria destructans
920
-
 Cyclobutanone pH 8.0, 25°C Ilyonectria destructans