Cloned (Comment) | Organism |
---|---|
gene vioD, recombinant expression of His-tagged enzyme in Escherichia coli | Chromobacterium violaceum |
Crystallization (Comment) | Organism |
---|---|
purified recombinant enzyme, sitting drop vapour diffusion method, mixing of 0.001 ml of 7.5-15 mg/ml protein in 20 mM TrisHCl pH 8.0, 300 mM NaCl, 10% glycerol, with 0.001 ml of reservoir solution containing 3.5 M sodium formate pH 7.0, and equilibration against 0.05 ml of reservoir solution, 2 weeks, X-ray diffraction structure determination and analysis at 1.7 A resolution, solvent-content calculation and molecular-replacement results suggest the presence of two molecules of VioD in the asymmetric unit | Chromobacterium violaceum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Chromobacterium violaceum | enzyme VioD is a flavin-dependent oxygenase that catalyzes the hydroxylation of the intermediate product prodeoxyviolaceinic acid at the 5-position of one indole ring to yield proviolacein | ? | - |
? | |
protodeoxyviolaceinate + NAD(P)H + H+ + O2 | Chromobacterium violaceum | - |
protoviolaceinate + NAD(P)+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chromobacterium violaceum | A0A024AX32 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography, ultrafiltration, and gel filtration | Chromobacterium violaceum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | enzyme VioD is a flavin-dependent oxygenase that catalyzes the hydroxylation of the intermediate product prodeoxyviolaceinic acid at the 5-position of one indole ring to yield proviolacein | Chromobacterium violaceum | ? | - |
? | |
protodeoxyviolaceinate + NAD(P)H + H+ + O2 | - |
Chromobacterium violaceum | protoviolaceinate + NAD(P)+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
VioD | - |
Chromobacterium violaceum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Chromobacterium violaceum | |
NADPH | - |
Chromobacterium violaceum |
General Information | Comment | Organism |
---|---|---|
metabolism | violacein, a natural purple secondary metabolite, is sequentially biosynthesized by five enzymes in the following pathway: VioA-VioB-VioE-VioD-VioC. VioD, a flavin-dependent oxygenase, catalyzes the hydroxylation of the intermediate product prodeoxyviolaceinic acid at the 5-position of one indole ring to yield proviolacein | Chromobacterium violaceum |