BRENDA - Enzyme Database show
show all sequences of 1.14.13.210

Biodegradation of 4-methyl-5-nitrocatechol by Pseudomonas sp. strain DNT

Haigler, B.E.; Nishino, S.F.; Spain, J.C.; J. Bacteriol. 176, 3433-3437 (1994)

Data extracted from this reference:

Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4-methyl-5-nitrocatechol + O2 + NADPH + H+
Pseudomonas sp.
-
2-hydroxy-5-methylquinone + nitrite + H2O + NADP+
-
-
?
4-methyl-5-nitrocatechol + O2 + NADPH + H+
Pseudomonas sp. DNT
-
2-hydroxy-5-methylquinone + nitrite + H2O + NADP+
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas sp.
-
-
-
Pseudomonas sp. DNT
-
-
-
Reaction
Reaction
Commentary
Organism
4-methyl-5-nitrocatechol + NAD(P)H + H+ + O2 = 2-hydroxy-5-methylquinone + nitrite + NAD(P)+ + H2O
mechanism of removal of the nitro group from 4-methyl-5-nitrocatechol and subsequent reactions leading to ring fission, overview
Pseudomonas sp.
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.03
-
strain DNT grown on yeast extract, pH and temperature not specified in the publication
Pseudomonas sp.
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-methyl-5-nitrocatechol + O2 + NADPH + H+
-
736336
Pseudomonas sp.
2-hydroxy-5-methylquinone + nitrite + H2O + NADP+
-
-
-
?
4-methyl-5-nitrocatechol + O2 + NADPH + H+
-
736336
Pseudomonas sp. DNT
2-hydroxy-5-methylquinone + nitrite + H2O + NADP+
-
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
-
Pseudomonas sp.
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
-
Pseudomonas sp.
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4-methyl-5-nitrocatechol + O2 + NADPH + H+
Pseudomonas sp.
-
2-hydroxy-5-methylquinone + nitrite + H2O + NADP+
-
-
?
4-methyl-5-nitrocatechol + O2 + NADPH + H+
Pseudomonas sp. DNT
-
2-hydroxy-5-methylquinone + nitrite + H2O + NADP+
-
-
?
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.03
-
strain DNT grown on yeast extract, pH and temperature not specified in the publication
Pseudomonas sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-methyl-5-nitrocatechol + O2 + NADPH + H+
-
736336
Pseudomonas sp.
2-hydroxy-5-methylquinone + nitrite + H2O + NADP+
-
-
-
?
4-methyl-5-nitrocatechol + O2 + NADPH + H+
-
736336
Pseudomonas sp. DNT
2-hydroxy-5-methylquinone + nitrite + H2O + NADP+
-
-
-
?
Other publictions for EC 1.14.13.210
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
735475
Leungsakul
Protein engineering of the 4-m ...
Burkholderia sp., Burkholderia sp. DNT
Appl. Environ. Microbiol.
72
3933-3939
2006
-
-
1
-
5
-
1
3
-
-
-
2
-
3
-
-
1
-
-
-
-
-
11
-
1
-
-
2
1
-
-
2
-
-
-
-
-
1
2
-
5
-
-
1
-
3
-
-
-
2
-
-
-
1
-
-
-
-
11
-
1
-
-
2
1
-
-
-
-
1
1
-
2
2
736341
Haigler
Purification and sequence anal ...
Burkholderia sp., Burkholderia sp. RASC
J. Bacteriol.
178
6019-6024
1996
-
-
1
-
-
-
-
-
-
-
2
-
-
3
-
-
1
-
-
-
-
-
6
1
-
-
-
-
-
-
-
3
-
-
-
-
-
1
3
-
-
-
-
-
-
-
-
-
2
-
-
-
-
1
-
-
-
-
6
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-
736336
Haigler
Biodegradation of 4-methyl-5-n ...
Pseudomonas sp., Pseudomonas sp. DNT
J. Bacteriol.
176
3433-3437
1994
-
-
-
-
-
-
-
-
-
-
-
2
-
5
-
-
-
1
-
-
1
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
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