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Literature summary for 1.14.13.210 extracted from

  • Haigler, B.E.; Nishino, S.F.; Spain, J.C.
    Biodegradation of 4-methyl-5-nitrocatechol by Pseudomonas sp. strain DNT (1994), J. Bacteriol., 176, 3433-3437.
    View publication on PubMedView publication on EuropePMC
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Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-methyl-5-nitrocatechol + O2 + NADPH + H+ Pseudomonas sp.
-
 2-hydroxy-5-methylquinone + nitrite + H2O + NADP+
-
 ?
4-methyl-5-nitrocatechol + O2 + NADPH + H+ Pseudomonas sp. DNT
-
 2-hydroxy-5-methylquinone + nitrite + H2O + NADP+
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-
Pseudomonas sp. DNT
-
-
-

Reaction

Reaction Comment Organism Reaction ID
4-methyl-5-nitrocatechol + NAD(P)H + H+ + O2 = 2-hydroxy-5-methylquinone + nitrite + NAD(P)+ + H2O mechanism of removal of the nitro group from 4-methyl-5-nitrocatechol and subsequent reactions leading to ring fission, overview Pseudomonas sp.
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.03
-
 strain DNT grown on yeast extract, pH and temperature not specified in the publication Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methyl-5-nitrocatechol + O2 + NADPH + H+
-
 Pseudomonas sp. 2-hydroxy-5-methylquinone + nitrite + H2O + NADP+
-
 ?
4-methyl-5-nitrocatechol + O2 + NADPH + H+
-
 Pseudomonas sp. DNT 2-hydroxy-5-methylquinone + nitrite + H2O + NADP+
-
 ?

Synonyms

Synonyms Comment Organism
MNC oxygenase
-
 Pseudomonas sp.

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Pseudomonas sp.