BRENDA - Enzyme Database show
show all sequences of 1.14.13.208

Structure and mechanism of the diiron benzoyl-coenzyme A epoxidase BoxB

Rather, L.J.; Weinert, T.; Demmer, U.; Bill, E.; Ismail, W.; Fuchs, G.; Ermler, U.; J. Biol. Chem. 286, 29241-29248 (2011)

Data extracted from this reference:

Crystallization (Commentary)
Crystallization
Organism
hanging drop vapor diffusion method, using 1.1 M NaH2PO4 and 0.2 M K2HPO4 as precipitant
Azoarcus evansii
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Fe2+
contains two irons per BoxB monomer
Azoarcus evansii
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
benzoyl-CoA + NADPH + H+ + O2
Azoarcus evansii
-
2,3-epoxy-2,3-dihydrobenzoyl-CoA + NADP+ + H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Azoarcus evansii
Q9AIX7
-
-
Purification (Commentary)
Commentary
Organism
Streptavidin affinity column chromatography and gel filtration
Azoarcus evansii
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
benzoyl-CoA + NADPH + H+ + O2
-
734168
Azoarcus evansii
2,3-epoxy-2,3-dihydrobenzoyl-CoA + NADP+ + H2O
-
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
-
Azoarcus evansii
[4Fe-4S]-center
-
Azoarcus evansii
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
-
Azoarcus evansii
[4Fe-4S]-center
-
Azoarcus evansii
Crystallization (Commentary) (protein specific)
Crystallization
Organism
hanging drop vapor diffusion method, using 1.1 M NaH2PO4 and 0.2 M K2HPO4 as precipitant
Azoarcus evansii
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Fe2+
contains two irons per BoxB monomer
Azoarcus evansii
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
benzoyl-CoA + NADPH + H+ + O2
Azoarcus evansii
-
2,3-epoxy-2,3-dihydrobenzoyl-CoA + NADP+ + H2O
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
Streptavidin affinity column chromatography and gel filtration
Azoarcus evansii
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
benzoyl-CoA + NADPH + H+ + O2
-
734168
Azoarcus evansii
2,3-epoxy-2,3-dihydrobenzoyl-CoA + NADP+ + H2O
-
-
-
?
Other publictions for EC 1.14.13.208
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
745167
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734168
Rather
Structure and mechanism of the ...
Azoarcus evansii
J. Biol. Chem.
286
29241-29248
2011
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1
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712441
Rather
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Azoarcus evansii, Azoarcus evansii KB740
J. Biol. Chem.
285
20615-20624
2010
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5
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10
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698566
Gescher
Genes coding for a new pathway ...
Azoarcus evansii
J. Bacteriol.
184
6301-6315
2002
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651677
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Reinvestigation of a new type ...
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J. Bacteriol.
183
1899-1908
2001
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1
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3
1
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3
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