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Literature summary for 1.14.13.208 extracted from

  • Rather, L.J.; Weinert, T.; Demmer, U.; Bill, E.; Ismail, W.; Fuchs, G.; Ermler, U.
    Structure and mechanism of the diiron benzoyl-coenzyme A epoxidase BoxB (2011), J. Biol. Chem., 286, 29241-29248.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 1.1 M NaH2PO4 and 0.2 M K2HPO4 as precipitant Aromatoleum evansii

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ contains two irons per BoxB monomer Aromatoleum evansii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
benzoyl-CoA + NADPH + H+ + O2 Aromatoleum evansii
-
2,3-epoxy-2,3-dihydrobenzoyl-CoA + NADP+ + H2O
-
?

Organism

Organism UniProt Comment Textmining
Aromatoleum evansii Q9AIX7
-
-

Purification (Commentary)

Purification (Comment) Organism
Streptavidin affinity column chromatography and gel filtration Aromatoleum evansii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzoyl-CoA + NADPH + H+ + O2
-
Aromatoleum evansii 2,3-epoxy-2,3-dihydrobenzoyl-CoA + NADP+ + H2O
-
?

Synonyms

Synonyms Comment Organism
benzoyl-coenzyme A epoxidase
-
Aromatoleum evansii
BoxA reductase subunit Aromatoleum evansii
BoxB epoxidase subunit Aromatoleum evansii

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Aromatoleum evansii
[4Fe-4S]-center
-
Aromatoleum evansii