BRENDA - Enzyme Database show
show all sequences of 1.14.13.208

Coenzyme A-dependent aerobic metabolism of benzoate via epoxide formation

Rather, L.J.; Knapp, B.; Haehnel, W.; Fuchs, G.; J. Biol. Chem. 285, 20615-20624 (2010)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
BoxC protein
-
Azoarcus evansii
Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli DH5alpha cells
Azoarcus evansii
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Fe2+
contains iron
Azoarcus evansii
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
54000
-
1 * 54000, BoxB, SDS-PAGE
Azoarcus evansii
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
benzoyl-CoA + NADPH + H+ + O2
Azoarcus evansii
-
2,3-epoxy-2,3-dihydrobenzoyl-CoA + NADP+ + H2O
-
-
?
benzoyl-CoA + NADPH + H+ + O2
Azoarcus evansii KB740
-
2,3-epoxy-2,3-dihydrobenzoyl-CoA + NADP+ + H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Azoarcus evansii
-
-
-
Azoarcus evansii KB740
-
-
-
Purification (Commentary)
Commentary
Organism
Strep-Tactin Superflow column chromatography
Azoarcus evansii
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
benzoyl-CoA + NADPH + H+ + O2
-
712441
Azoarcus evansii
2,3-epoxy-2,3-dihydrobenzoyl-CoA + NADP+ + H2O
-
-
-
?
benzoyl-CoA + NADPH + H+ + O2
-
712441
Azoarcus evansii KB740
2,3-epoxy-2,3-dihydrobenzoyl-CoA + NADP+ + H2O
-
-
-
?
Subunits
Subunits
Commentary
Organism
monomer
1 * 54000, BoxB, SDS-PAGE
Azoarcus evansii
Cofactor
Cofactor
Commentary
Organism
Structure
FAD
-
Azoarcus evansii
Fe-S center
-
Azoarcus evansii
NADPH
-
Azoarcus evansii
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
BoxC protein
-
Azoarcus evansii
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli DH5alpha cells
Azoarcus evansii
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
FAD
-
Azoarcus evansii
Fe-S center
-
Azoarcus evansii
NADPH
-
Azoarcus evansii
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Fe2+
contains iron
Azoarcus evansii
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
54000
-
1 * 54000, BoxB, SDS-PAGE
Azoarcus evansii
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
benzoyl-CoA + NADPH + H+ + O2
Azoarcus evansii
-
2,3-epoxy-2,3-dihydrobenzoyl-CoA + NADP+ + H2O
-
-
?
benzoyl-CoA + NADPH + H+ + O2
Azoarcus evansii KB740
-
2,3-epoxy-2,3-dihydrobenzoyl-CoA + NADP+ + H2O
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
Strep-Tactin Superflow column chromatography
Azoarcus evansii
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
benzoyl-CoA + NADPH + H+ + O2
-
712441
Azoarcus evansii
2,3-epoxy-2,3-dihydrobenzoyl-CoA + NADP+ + H2O
-
-
-
?
benzoyl-CoA + NADPH + H+ + O2
-
712441
Azoarcus evansii KB740
2,3-epoxy-2,3-dihydrobenzoyl-CoA + NADP+ + H2O
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 54000, BoxB, SDS-PAGE
Azoarcus evansii
Other publictions for EC 1.14.13.208
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
745167
Rokob
Pathways for arene oxidation ...
Azoarcus evansii, Azoarcus evansii KB 740
J. Am. Chem. Soc.
138
14623-14638
2016
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1
1
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744692
Liao
Mechanism and selectivity of ...
Azoarcus evansii
Chem. Sci.
6
2754-2764
2015
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1
1
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733379
Rather
The reducing component BoxA of ...
Azoarcus evansii
Biochim. Biophys. Acta
1814
1609-1615
2011
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734168
Rather
Structure and mechanism of the ...
Azoarcus evansii
J. Biol. Chem.
286
29241-29248
2011
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712441
Rather
Coenzyme A-dependent aerobic m ...
Azoarcus evansii, Azoarcus evansii KB740
J. Biol. Chem.
285
20615-20624
2010
1
-
1
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1
1
2
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5
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1
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1
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700258
Zaar
New enzymes involved in aerobi ...
Azoarcus evansii, Azoarcus evansii KB740
Mol. Microbiol.
54
223-238
2004
1
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1
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1
1
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4
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1
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10
1
1
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10
1
1
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1
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1
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698566
Gescher
Genes coding for a new pathway ...
Azoarcus evansii
J. Bacteriol.
184
6301-6315
2002
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1
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1
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1
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3
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651677
Mohamed
Reinvestigation of a new type ...
Azoarcus evansii
J. Bacteriol.
183
1899-1908
2001
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1
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1
3
1
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3
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