BRENDA - Enzyme Database
show all sequences of 1.14.13.200

Cloning, sequencing, and heterologous expression of the elmGHIJ genes involved in the biosynthesis of the polyketide antibiotic elloramycin from Streptomyces olivaceus Tue2353

Rafanan, E.R.; Le, L.; Zhao, L.; Decker, H.; Shen, B.; J. Nat. Prod. 64, 444-449 (2001)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expression in Escherichia coli and Streptomyces lividans
Streptomyces olivaceus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
58000
-
x * 58085, clalculated, x * 58000, SDS-PAGE
Streptomyces olivaceus
58085
-
x * 58085, clalculated, x * 58000, SDS-PAGE
Streptomyces olivaceus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
tetracenomycin B2 + 2 O2 + 2 NAD(P)H + 2 H+
Streptomyces olivaceus
natural reaction in Streptomyces olivaceus
8-demethyl tetracenomycin C + 2 NAD(P)+ + H2O
-
-
?
tetracenomycin B2 + 2 O2 + 2 NAD(P)H + 2 H+
Streptomyces olivaceus Tue2353
natural reaction in Streptomyces olivaceus
8-demethyl tetracenomycin C + 2 NAD(P)+ + H2O
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Streptomyces olivaceus
Q9L4Y1
-
-
Streptomyces olivaceus Tue2353
Q9L4Y1
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
tetracenomycin A2 + 2 O2 + 2 NAD(P)H + 2 H+
-
730232
Streptomyces olivaceus
tetracenomycin C + 2 NAD(P)+ + H2O
quantitative conversion
-
-
?
tetracenomycin A2 + 2 O2 + 2 NAD(P)H + 2 H+
-
730232
Streptomyces olivaceus Tue2353
tetracenomycin C + 2 NAD(P)+ + H2O
quantitative conversion
-
-
?
tetracenomycin B2 + 2 O2 + 2 NAD(P)H + 2 H+
natural reaction in Streptomyces olivaceus
730232
Streptomyces olivaceus
8-demethyl tetracenomycin C + 2 NAD(P)+ + H2O
-
-
-
?
tetracenomycin B2 + 2 O2 + 2 NAD(P)H + 2 H+
natural reaction in Streptomyces olivaceus
730232
Streptomyces olivaceus Tue2353
8-demethyl tetracenomycin C + 2 NAD(P)+ + H2O
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 58085, clalculated, x * 58000, SDS-PAGE
Streptomyces olivaceus
Synonyms
Synonyms
Commentary
Organism
ElmG
-
Streptomyces olivaceus
Tcm B2 oxygenase
-
Streptomyces olivaceus
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Streptomyces olivaceus
calculated
-
6
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli and Streptomyces lividans
Streptomyces olivaceus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
58000
-
x * 58085, clalculated, x * 58000, SDS-PAGE
Streptomyces olivaceus
58085
-
x * 58085, clalculated, x * 58000, SDS-PAGE
Streptomyces olivaceus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
tetracenomycin B2 + 2 O2 + 2 NAD(P)H + 2 H+
Streptomyces olivaceus
natural reaction in Streptomyces olivaceus
8-demethyl tetracenomycin C + 2 NAD(P)+ + H2O
-
-
?
tetracenomycin B2 + 2 O2 + 2 NAD(P)H + 2 H+
Streptomyces olivaceus Tue2353
natural reaction in Streptomyces olivaceus
8-demethyl tetracenomycin C + 2 NAD(P)+ + H2O
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
tetracenomycin A2 + 2 O2 + 2 NAD(P)H + 2 H+
-
730232
Streptomyces olivaceus
tetracenomycin C + 2 NAD(P)+ + H2O
quantitative conversion
-
-
?
tetracenomycin A2 + 2 O2 + 2 NAD(P)H + 2 H+
-
730232
Streptomyces olivaceus Tue2353
tetracenomycin C + 2 NAD(P)+ + H2O
quantitative conversion
-
-
?
tetracenomycin B2 + 2 O2 + 2 NAD(P)H + 2 H+
natural reaction in Streptomyces olivaceus
730232
Streptomyces olivaceus
8-demethyl tetracenomycin C + 2 NAD(P)+ + H2O
-
-
-
?
tetracenomycin B2 + 2 O2 + 2 NAD(P)H + 2 H+
natural reaction in Streptomyces olivaceus
730232
Streptomyces olivaceus Tue2353
8-demethyl tetracenomycin C + 2 NAD(P)+ + H2O
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 58085, clalculated, x * 58000, SDS-PAGE
Streptomyces olivaceus
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Streptomyces olivaceus
calculated
-
6
Other publictions for EC 1.14.13.200
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
730232
Rafanan
Cloning, sequencing, and heter ...
Streptomyces olivaceus, Streptomyces olivaceus Tue2353
J. Nat. Prod.
64
444-449
2001
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1
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728863
Beynon
Crystallization and preliminar ...
Streptomyces glaucescens
Acta Crystallogr. Sect. D
56
1647-1651
2000
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730547
Rafanan
Triple hydroxylation of tetrac ...
Streptomyces glaucescens, Streptomyces olivaceus, Streptomyces olivaceus Tue2353
Org. Lett.
2
3225-3227
2000
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727813
Shen
Triple hydroxylation of tetrac ...
Streptomyces glaucescens
J. Biol. Chem.
269
30726-30733
1994
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5
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1
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729909
Decker
Nucleotide sequences and heter ...
Streptomyces glaucescens
J. Bacteriol.
175
3876-3886
1993
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