BRENDA - Enzyme Database show
show all sequences of 1.14.13.200

Triple hydroxylation of tetracenomycin A2 to tetracenomycin C in Streptomyces glaucescens. Overexpression of the tcmG gene in Streptomyces lividans and characterization of the tetracenomycin A2 oxygenase

Shen, B.; Hutchinson, C.R.; J. Biol. Chem. 269, 30726-30733 (1994)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
overexpression in Streptomyces lividans
Streptomyces glaucescens
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0018
-
tetracenomycin A2
pH 9.0, 25°C
Streptomyces glaucescens
0.082
-
NADH
pH 9.0, 25°C
Streptomyces glaucescens
0.26
-
NADPH
pH 9.0, 25°C
Streptomyces glaucescens
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
60000
-
1 * 60000, SDS-PAGE
Streptomyces glaucescens
61000
-
gel filtration
Streptomyces glaucescens
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
tetracenomycin A2 + 2 O2 + 2 NAD(P)H + 2 H+
Streptomyces glaucescens
biosynthesis tetracenomycin C, a polyketide antitumor antibiotic
tetracenomycin C + 2 NAD(P)+ + H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Streptomyces glaucescens
Q7M0K6
-
-
Purification (Commentary)
Commentary
Organism
-
Streptomyces glaucescens
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.00429
-
pH 9.0, 25°C
Streptomyces glaucescens
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
tetracenomycin A2 + 2 O2 + 2 NAD(P)H + 2 H+
biosynthesis tetracenomycin C, a polyketide antitumor antibiotic
727813
Streptomyces glaucescens
tetracenomycin C + 2 NAD(P)+ + H2O
-
-
-
?
tetracenomycin A2 + 2 O2 + 2 NADH + 2 H+
tetracenomycin A2 i.e. methyl 10,12-dihydroxy-3,8-dimethoxy-1-methyl-6,11-dioxo-6,11-dihydrotetracene-2-carboxylate. The enzyme prefers NADPH over NADH as an electron donor
727813
Streptomyces glaucescens
tetracenomycin C + 2 NAD+ + H2O
tetracenomycin C = methyl (6aR,7S,10aR)-6a,7,10a,12-tetrahydroxy-3,8-dimethoxy-1-methyl-6,10,11-trioxo-6,6a,7,10,10a,11-hexahydrotetracene-2-carboxylate
-
-
?
tetracenomycin A2 + 2 O2 + 2 NADPH + 2 H+
tetracenomycin A2 i.e. methyl 10,12-dihydroxy-3,8-dimethoxy-1-methyl-6,11-dioxo-6,11-dihydrotetracene-2-carboxylate. The enzyme prefers NADPH over NADH as an electron donor
727813
Streptomyces glaucescens
tetracenomycin C + 2 NADP+ + H2O
tetracenomycin C = methyl (6aR,7S,10aR)-6a,7,10a,12-tetrahydroxy-3,8-dimethoxy-1-methyl-6,10,11-trioxo-6,6a,7,10,10a,11-hexahydrotetracene-2-carboxylate
-
-
?
Subunits
Subunits
Commentary
Organism
monomer
1 * 60000, SDS-PAGE
Streptomyces glaucescens
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
9.5
in 50 mM Tris-HC1 or 50 nm ethanolamine-HCl buffer
Streptomyces glaucescens
pH Range
pH Minimum
pH Maximum
Commentary
Organism
7.5
10
pH 7.5: about 45% of maximal activity, pH 10: about 25% of maximal activity
Streptomyces glaucescens
Cofactor
Cofactor
Commentary
Organism
Structure
FAD
contains 1 mol of non-covalently bound FAD. The apoenzyme can be partially reconstituted in vitro by addition of FAD
Streptomyces glaucescens
Cloned(Commentary) (protein specific)
Commentary
Organism
overexpression in Streptomyces lividans
Streptomyces glaucescens
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
FAD
contains 1 mol of non-covalently bound FAD. The apoenzyme can be partially reconstituted in vitro by addition of FAD
Streptomyces glaucescens
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0018
-
tetracenomycin A2
pH 9.0, 25°C
Streptomyces glaucescens
0.082
-
NADH
pH 9.0, 25°C
Streptomyces glaucescens
0.26
-
NADPH
pH 9.0, 25°C
Streptomyces glaucescens
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
60000
-
1 * 60000, SDS-PAGE
Streptomyces glaucescens
61000
-
gel filtration
Streptomyces glaucescens
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
tetracenomycin A2 + 2 O2 + 2 NAD(P)H + 2 H+
Streptomyces glaucescens
biosynthesis tetracenomycin C, a polyketide antitumor antibiotic
tetracenomycin C + 2 NAD(P)+ + H2O
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Streptomyces glaucescens
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.00429
-
pH 9.0, 25°C
Streptomyces glaucescens
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
tetracenomycin A2 + 2 O2 + 2 NAD(P)H + 2 H+
biosynthesis tetracenomycin C, a polyketide antitumor antibiotic
727813
Streptomyces glaucescens
tetracenomycin C + 2 NAD(P)+ + H2O
-
-
-
?
tetracenomycin A2 + 2 O2 + 2 NADH + 2 H+
tetracenomycin A2 i.e. methyl 10,12-dihydroxy-3,8-dimethoxy-1-methyl-6,11-dioxo-6,11-dihydrotetracene-2-carboxylate. The enzyme prefers NADPH over NADH as an electron donor
727813
Streptomyces glaucescens
tetracenomycin C + 2 NAD+ + H2O
tetracenomycin C = methyl (6aR,7S,10aR)-6a,7,10a,12-tetrahydroxy-3,8-dimethoxy-1-methyl-6,10,11-trioxo-6,6a,7,10,10a,11-hexahydrotetracene-2-carboxylate
-
-
?
tetracenomycin A2 + 2 O2 + 2 NADPH + 2 H+
tetracenomycin A2 i.e. methyl 10,12-dihydroxy-3,8-dimethoxy-1-methyl-6,11-dioxo-6,11-dihydrotetracene-2-carboxylate. The enzyme prefers NADPH over NADH as an electron donor
727813
Streptomyces glaucescens
tetracenomycin C + 2 NADP+ + H2O
tetracenomycin C = methyl (6aR,7S,10aR)-6a,7,10a,12-tetrahydroxy-3,8-dimethoxy-1-methyl-6,10,11-trioxo-6,6a,7,10,10a,11-hexahydrotetracene-2-carboxylate
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 60000, SDS-PAGE
Streptomyces glaucescens
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
9.5
in 50 mM Tris-HC1 or 50 nm ethanolamine-HCl buffer
Streptomyces glaucescens
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
7.5
10
pH 7.5: about 45% of maximal activity, pH 10: about 25% of maximal activity
Streptomyces glaucescens
Other publictions for EC 1.14.13.200
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
730232
Rafanan
Cloning, sequencing, and heter ...
Streptomyces olivaceus, Streptomyces olivaceus Tue2353
J. Nat. Prod.
64
444-449
2001
-
-
1
-
-
-
-
-
-
-
2
2
-
3
-
-
-
-
-
-
-
-
4
1
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
-
-
-
-
-
4
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
728863
Beynon
Crystallization and preliminar ...
Streptomyces glaucescens
Acta Crystallogr. Sect. D
56
1647-1651
2000
-
-
-
1
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
730547
Rafanan
Triple hydroxylation of tetrac ...
Streptomyces glaucescens, Streptomyces olivaceus, Streptomyces olivaceus Tue2353
Org. Lett.
2
3225-3227
2000
-
-
2
-
-
-
-
-
-
-
-
-
-
4
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
727813
Shen
Triple hydroxylation of tetrac ...
Streptomyces glaucescens
J. Biol. Chem.
269
30726-30733
1994
-
-
1
-
-
-
-
3
-
-
2
1
-
5
-
-
1
-
-
-
1
-
3
1
-
-
-
-
1
1
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
3
-
-
2
1
-
-
-
1
-
-
1
-
3
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
729909
Decker
Nucleotide sequences and heter ...
Streptomyces glaucescens
J. Bacteriol.
175
3876-3886
1993
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-