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Literature summary for 1.14.13.20 extracted from

  • Huong, N.L.; Itoh, K.; Miyamoto, M.; Suyama, K.; Yamamoto, H.
    Chlorophenol hydroxylase activity encoded by TfdB from 2,4-dichlorophenoxyacetic acid (2,4-D)-degrading Bradyrhizobium sp. strain RD5-C2 (2007), Biosci. Biotechnol. Biochem., 71, 1691-1696.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene tfdBa, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree, expression in Escherichia coli strain BL21, subcloning in Escherichia coli strains JM109 amd T7 Blue Bradyrhizobium sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2,4-dichlorophenol + NADPH + H+ + O2 Bradyrhizobium sp. best substrate 3,5-dichlorocatechol + NADP+ + H2O
-
?
2,4-dichlorophenol + NADPH + H+ + O2 Bradyrhizobium sp. RD5-C2 best substrate 3,5-dichlorocatechol + NADP+ + H2O
-
?

Organism

Organism UniProt Comment Textmining
Bradyrhizobium sp. A5LGH2 gene tfdBa
-
Bradyrhizobium sp. RD5-C2 A5LGH2 gene tfdBa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,3-dichlorophenol + NADPH + H+ + O2 26% activity compared to 2,4-dichlorophenol as substrate Bradyrhizobium sp. ? + NADP+ + H2O
-
?
2,3-dichlorophenol + NADPH + H+ + O2 26% activity compared to 2,4-dichlorophenol as substrate Bradyrhizobium sp. RD5-C2 ? + NADP+ + H2O
-
?
2,4-dichlorophenol + NADPH + H+ + O2 best substrate Bradyrhizobium sp. 3,5-dichlorocatechol + NADP+ + H2O
-
?
2,4-dichlorophenol + NADPH + H+ + O2 best substrate Bradyrhizobium sp. RD5-C2 3,5-dichlorocatechol + NADP+ + H2O
-
?
3,4-dichlorophenol + NADPH + H+ + O2 30% activity compared to 2,4-dichlorophenol as substrate Bradyrhizobium sp. ? + NADP+ + H2O
-
?
3,4-dichlorophenol + NADPH + H+ + O2 30% activity compared to 2,4-dichlorophenol as substrate Bradyrhizobium sp. RD5-C2 ? + NADP+ + H2O
-
?
4-chlorophenol + NADPH + H+ + O2 68% activity compared to 2,4-dichlorophenol as substrate Bradyrhizobium sp. ? + NADP+ + H2O
-
?
4-chlorophenol + NADPH + H+ + O2 68% activity compared to 2,4-dichlorophenol as substrate Bradyrhizobium sp. RD5-C2 ? + NADP+ + H2O
-
?
additional information the enzyme shows a narrow substrate specificity with chlorophenols, 3,5-dichlorophenol is a poor substrate, overview Bradyrhizobium sp. ?
-
?
additional information the enzyme shows a narrow substrate specificity with chlorophenols, 3,5-dichlorophenol is a poor substrate, overview Bradyrhizobium sp. RD5-C2 ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Bradyrhizobium sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
assay at Bradyrhizobium sp.

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Bradyrhizobium sp.