BRENDA - Enzyme Database show
show all sequences of 1.14.13.20

Chlorophenol hydroxylase activity encoded by TfdB from 2,4-dichlorophenoxyacetic acid (2,4-D)-degrading Bradyrhizobium sp. strain RD5-C2

Huong, N.L.; Itoh, K.; Miyamoto, M.; Suyama, K.; Yamamoto, H.; Biosci. Biotechnol. Biochem. 71, 1691-1696 (2007)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene tfdBa, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree, expression in Escherichia coli strain BL21, subcloning in Escherichia coli strains JM109 amd T7 Blue
Bradyrhizobium sp.
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2,4-dichlorophenol + NADPH + H+ + O2
Bradyrhizobium sp.
best substrate
3,5-dichlorocatechol + NADP+ + H2O
-
-
?
2,4-dichlorophenol + NADPH + H+ + O2
Bradyrhizobium sp. RD5-C2
best substrate
3,5-dichlorocatechol + NADP+ + H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Bradyrhizobium sp.
A5LGH2
gene tfdBa
-
Bradyrhizobium sp. RD5-C2
A5LGH2
gene tfdBa
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2,3-dichlorophenol + NADPH + H+ + O2
26% activity compared to 2,4-dichlorophenol as substrate
685661
Bradyrhizobium sp.
? + NADP+ + H2O
-
-
-
?
2,3-dichlorophenol + NADPH + H+ + O2
26% activity compared to 2,4-dichlorophenol as substrate
685661
Bradyrhizobium sp. RD5-C2
? + NADP+ + H2O
-
-
-
?
2,4-dichlorophenol + NADPH + H+ + O2
best substrate
685661
Bradyrhizobium sp.
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
?
2,4-dichlorophenol + NADPH + H+ + O2
best substrate
685661
Bradyrhizobium sp. RD5-C2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
?
3,4-dichlorophenol + NADPH + H+ + O2
30% activity compared to 2,4-dichlorophenol as substrate
685661
Bradyrhizobium sp.
? + NADP+ + H2O
-
-
-
?
3,4-dichlorophenol + NADPH + H+ + O2
30% activity compared to 2,4-dichlorophenol as substrate
685661
Bradyrhizobium sp. RD5-C2
? + NADP+ + H2O
-
-
-
?
4-chlorophenol + NADPH + H+ + O2
68% activity compared to 2,4-dichlorophenol as substrate
685661
Bradyrhizobium sp.
? + NADP+ + H2O
-
-
-
?
4-chlorophenol + NADPH + H+ + O2
68% activity compared to 2,4-dichlorophenol as substrate
685661
Bradyrhizobium sp. RD5-C2
? + NADP+ + H2O
-
-
-
?
additional information
the enzyme shows a narrow substrate specificity with chlorophenols, 3,5-dichlorophenol is a poor substrate, overview
685661
Bradyrhizobium sp.
?
-
-
-
-
additional information
the enzyme shows a narrow substrate specificity with chlorophenols, 3,5-dichlorophenol is a poor substrate, overview
685661
Bradyrhizobium sp. RD5-C2
?
-
-
-
-
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
assay at
Bradyrhizobium sp.
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.6
-
assay at
Bradyrhizobium sp.
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
-
Bradyrhizobium sp.
Cloned(Commentary) (protein specific)
Commentary
Organism
gene tfdBa, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree, expression in Escherichia coli strain BL21, subcloning in Escherichia coli strains JM109 amd T7 Blue
Bradyrhizobium sp.
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
-
Bradyrhizobium sp.
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2,4-dichlorophenol + NADPH + H+ + O2
Bradyrhizobium sp.
best substrate
3,5-dichlorocatechol + NADP+ + H2O
-
-
?
2,4-dichlorophenol + NADPH + H+ + O2
Bradyrhizobium sp. RD5-C2
best substrate
3,5-dichlorocatechol + NADP+ + H2O
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2,3-dichlorophenol + NADPH + H+ + O2
26% activity compared to 2,4-dichlorophenol as substrate
685661
Bradyrhizobium sp.
? + NADP+ + H2O
-
-
-
?
2,3-dichlorophenol + NADPH + H+ + O2
26% activity compared to 2,4-dichlorophenol as substrate
685661
Bradyrhizobium sp. RD5-C2
? + NADP+ + H2O
-
-
-
?
2,4-dichlorophenol + NADPH + H+ + O2
best substrate
685661
Bradyrhizobium sp.
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
?
2,4-dichlorophenol + NADPH + H+ + O2
best substrate
685661
Bradyrhizobium sp. RD5-C2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
?
3,4-dichlorophenol + NADPH + H+ + O2
30% activity compared to 2,4-dichlorophenol as substrate
685661
Bradyrhizobium sp.
? + NADP+ + H2O
-
-
-
?
3,4-dichlorophenol + NADPH + H+ + O2
30% activity compared to 2,4-dichlorophenol as substrate
685661
Bradyrhizobium sp. RD5-C2
? + NADP+ + H2O
-
-
-
?
4-chlorophenol + NADPH + H+ + O2
68% activity compared to 2,4-dichlorophenol as substrate
685661
Bradyrhizobium sp.
? + NADP+ + H2O
-
-
-
?
4-chlorophenol + NADPH + H+ + O2
68% activity compared to 2,4-dichlorophenol as substrate
685661
Bradyrhizobium sp. RD5-C2
? + NADP+ + H2O
-
-
-
?
additional information
the enzyme shows a narrow substrate specificity with chlorophenols, 3,5-dichlorophenol is a poor substrate, overview
685661
Bradyrhizobium sp.
?
-
-
-
-
additional information
the enzyme shows a narrow substrate specificity with chlorophenols, 3,5-dichlorophenol is a poor substrate, overview
685661
Bradyrhizobium sp. RD5-C2
?
-
-
-
-
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
assay at
Bradyrhizobium sp.
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.6
-
assay at
Bradyrhizobium sp.
Other publictions for EC 1.14.13.20
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
742330
Xia
Biodegradation of the Herbici ...
Achromobacter sp. LZ35
Curr. Microbiol.
74
193-202
2017
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1
1
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-
-
724636
Lu
Cloning and characterisation o ...
Soil bacterium
Biotechnol. Lett.
33
1159-1167
2011
-
-
1
-
-
-
3
2
-
3
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-
-
3
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-
-
-
-
-
-
-
9
-
1
-
-
-
1
-
-
-
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-
-
-
1
-
-
-
-
-
3
-
2
-
3
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-
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-
9
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
685661
Huong
Chlorophenol hydroxylase activ ...
Bradyrhizobium sp., Bradyrhizobium sp. RD5-C2
Biosci. Biotechnol. Biochem.
71
1691-1696
2007
-
-
1
-
-
-
-
-
-
-
-
2
-
5
-
-
-
-
-
-
-
-
10
-
1
-
-
-
1
-
-
1
-
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-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
10
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
671456
Ledger
Chlorophenol hydroxylases enco ...
Cupriavidus necator, Paraburkholderia xenovorans
Appl. Environ. Microbiol.
72
2783-2792
2006
-
-
-
-
2
-
-
1
-
-
-
8
-
7
-
-
-
-
-
-
1
-
8
-
2
-
-
-
2
-
-
2
-
-
-
-
-
-
2
-
2
-
-
-
-
1
-
-
-
8
-
-
-
-
-
-
1
-
8
-
2
-
-
-
2
-
-
-
-
-
-
-
-
-
439014
Farhana
Monoclonal antibodies to 2,4-d ...
Burkholderia cepacia, Burkholderia cepacia DBO131, Burkholderia mallei, Burkholderia mallei TFD6, Burkholderia sp., Burkholderia sp. RASC, Burkholderia sp. TFD2, Cupriavidus necator, Cupriavidus necator JMP 134-1, Cupriavidus necator TFD41, Pseudomonas putida, Pseudomonas putida PaW85, Rhodoferax fermentans, Rhodoferax fermentans B6-9, Rhodoferax fermentans TFD23, Rhodopseudomonas palustris, Rhodopseudomonas palustris M1, Sphingomonas paucimobilis 1443, Sphingomonas paucimobilis, Sphingomonas sp., Sphingomonas sp. B6-10, Sphingomonas sp. B6-5, Sphingomonas sp. EML146, Sphingomonas sp. TFD44, Variovorax paradoxus 2811P, Variovorax paradoxus
Can. J. Microbiol.
44
920-928
1998
-
-
-
-
-
-
-
-
-
-
-
26
-
54
-
-
1
-
-
-
-
-
26
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
26
-
-
-
1
-
-
-
-
26
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
439015
Farhana
The 2,4-dichlorophenol hydroxy ...
Cupriavidus necator, Cupriavidus necator JMP 134-1
Can. J. Microbiol.
43
202-205
1997
-
-
-
-
-
-
-
-
-
-
2
2
-
28
-
-
1
-
-
-
1
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
1
-
-
1
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
439016
Makdessi
Purification and characterizat ...
Proteobacteria, Proteobacteria S1
FEMS Microbiol. Lett.
157
95-101
1997
-
-
-
-
-
-
-
3
-
-
2
2
-
5
-
-
1
-
-
-
2
-
20
1
-
-
-
-
-
-
-
3
-
-
-
-
-
-
3
-
-
-
-
-
-
3
-
-
2
2
-
-
-
1
-
-
2
-
20
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
439013
Radjendirane
Affinity purification and char ...
Burkholderia cepacia
Arch. Biochem. Biophys.
288
169-176
1991
-
-
-
-
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-
8
2
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2
1
-
3
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1
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1
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1
1
1
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1
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3
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3
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8
-
2
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2
1
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-
1
-
-
1
-
1
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
439012
Liu
Purification and properties of ...
Cupriavidus necator, Cupriavidus necator JMP 134-1
FEBS Lett.
173
314-318
1984
-
-
1
-
-
-
11
4
-
-
3
2
-
27
-
-
1
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-
-
1
-
13
1
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-
-
2
-
-
3
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-
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-
-
1
3
-
-
-
-
11
-
4
-
-
3
2
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-
1
-
-
1
-
13
1
-
-
-
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2
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-
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-
-
439011
Beadle
The purification and propertie ...
Acinetobacter sp.
Eur. J. Biochem.
123
323-332
1982
-
-
-
-
-
4
10
3
-
-
2
1
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3
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1
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1
1
7
1
1
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1
1
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3
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3
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-
4
-
10
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3
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2
1
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1
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1
1
7
1
1
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1
1
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