BRENDA - Enzyme Database show
show all sequences of 1.14.13.20

Affinity purification and characterization of 2,4-dichlorophenol hydroxylase from Pseudomonas cepacia [published erratum appears in Arch Biochem Biophys 1992 Jul;296(1):354]

Radjendirane, V.; Bhat, M.A.; Vaidyanathan, C.S.; Arch. Biochem. Biophys. 288, 169-176 (1991)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
2,4,5-Trichlorophenol
-
Burkholderia cepacia
2,4,6-Trichlorophenol
-
Burkholderia cepacia
guanidine hydrochloride
-
Burkholderia cepacia
Hg2+
-
Burkholderia cepacia
p-chloromercuribenzoate
-
Burkholderia cepacia
SDS
-
Burkholderia cepacia
Urea
-
Burkholderia cepacia
Zn2+
-
Burkholderia cepacia
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.014
-
2,4-Dichlorophenol
-
Burkholderia cepacia
0.1
-
NADPH
-
Burkholderia cepacia
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
69000
-
alpha4, 4 * 69000, SDS-PAGE
Burkholderia cepacia
275000
-
gel filtration and ultracentrifugation
Burkholderia cepacia
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2,4-dichlorophenol + NADPH + O2
Burkholderia cepacia
-
3,5-dichlorocatechol + NADP+ + H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Burkholderia cepacia
-
-
-
Purification (Commentary)
Commentary
Organism
single step affinity chromatography
Burkholderia cepacia
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.9
-
-
Burkholderia cepacia
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2,4-dichlorophenol + NADPH + O2
-
439013
Burkholderia cepacia
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
?
Subunits
Subunits
Commentary
Organism
tetramer
alpha4, 4 * 69000, SDS-PAGE
Burkholderia cepacia
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
-
Burkholderia cepacia
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
-
Burkholderia cepacia
Cofactor
Cofactor
Commentary
Organism
Structure
FAD
1 mol of enzyme contains: 4 mol FAD; flavoprotein; requires exogenous addition of FAD
Burkholderia cepacia
NADH
NADPH preferred to NADH
Burkholderia cepacia
NADPH
-
Burkholderia cepacia
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
FAD
1 mol of enzyme contains: 4 mol FAD; flavoprotein; requires exogenous addition of FAD
Burkholderia cepacia
NADH
NADPH preferred to NADH
Burkholderia cepacia
NADPH
-
Burkholderia cepacia
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
2,4,5-Trichlorophenol
-
Burkholderia cepacia
2,4,6-Trichlorophenol
-
Burkholderia cepacia
guanidine hydrochloride
-
Burkholderia cepacia
Hg2+
-
Burkholderia cepacia
p-chloromercuribenzoate
-
Burkholderia cepacia
SDS
-
Burkholderia cepacia
Urea
-
Burkholderia cepacia
Zn2+
-
Burkholderia cepacia
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.014
-
2,4-Dichlorophenol
-
Burkholderia cepacia
0.1
-
NADPH
-
Burkholderia cepacia
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
69000
-
alpha4, 4 * 69000, SDS-PAGE
Burkholderia cepacia
275000
-
gel filtration and ultracentrifugation
Burkholderia cepacia
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2,4-dichlorophenol + NADPH + O2
Burkholderia cepacia
-
3,5-dichlorocatechol + NADP+ + H2O
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
single step affinity chromatography
Burkholderia cepacia
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.9
-
-
Burkholderia cepacia
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2,4-dichlorophenol + NADPH + O2
-
439013
Burkholderia cepacia
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
tetramer
alpha4, 4 * 69000, SDS-PAGE
Burkholderia cepacia
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
-
Burkholderia cepacia
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
-
Burkholderia cepacia
Other publictions for EC 1.14.13.20
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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1
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724636
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3
2
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3
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3
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9
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1
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1
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1
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3
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2
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3
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9
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1
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1
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685661
Huong
Chlorophenol hydroxylase activ ...
Bradyrhizobium sp., Bradyrhizobium sp. RD5-C2
Biosci. Biotechnol. Biochem.
71
1691-1696
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-
1
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2
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5
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10
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1
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1
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1
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1
1
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2
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-
10
-
1
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1
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-
-
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-
671456
Ledger
Chlorophenol hydroxylases enco ...
Cupriavidus necator, Paraburkholderia xenovorans
Appl. Environ. Microbiol.
72
2783-2792
2006
-
-
-
-
2
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1
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8
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7
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1
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8
-
2
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2
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-
2
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2
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2
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1
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8
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1
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8
-
2
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-
2
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-
-
-
-
-
439014
Farhana
Monoclonal antibodies to 2,4-d ...
Burkholderia cepacia, Burkholderia cepacia DBO131, Burkholderia mallei, Burkholderia mallei TFD6, Burkholderia sp., Burkholderia sp. RASC, Burkholderia sp. TFD2, Cupriavidus necator, Cupriavidus necator JMP 134-1, Cupriavidus necator TFD41, Pseudomonas putida, Pseudomonas putida PaW85, Rhodoferax fermentans, Rhodoferax fermentans B6-9, Rhodoferax fermentans TFD23, Rhodopseudomonas palustris, Rhodopseudomonas palustris M1, Sphingomonas paucimobilis 1443, Sphingomonas paucimobilis, Sphingomonas sp., Sphingomonas sp. B6-10, Sphingomonas sp. B6-5, Sphingomonas sp. EML146, Sphingomonas sp. TFD44, Variovorax paradoxus 2811P, Variovorax paradoxus
Can. J. Microbiol.
44
920-928
1998
-
-
-
-
-
-
-
-
-
-
-
26
-
54
-
-
1
-
-
-
-
-
26
-
-
-
-
-
-
-
-
-
-
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-
-
-
-
-
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-
-
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-
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-
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26
-
-
-
1
-
-
-
-
26
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
439015
Farhana
The 2,4-dichlorophenol hydroxy ...
Cupriavidus necator, Cupriavidus necator JMP 134-1
Can. J. Microbiol.
43
202-205
1997
-
-
-
-
-
-
-
-
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-
2
2
-
28
-
-
1
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-
1
-
2
1
-
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2
2
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1
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-
1
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
439016
Makdessi
Purification and characterizat ...
Proteobacteria, Proteobacteria S1
FEMS Microbiol. Lett.
157
95-101
1997
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-
-
-
-
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-
3
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-
2
2
-
5
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-
1
-
-
-
2
-
20
1
-
-
-
-
-
-
-
3
-
-
-
-
-
-
3
-
-
-
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-
-
3
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2
2
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-
1
-
-
2
-
20
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
439013
Radjendirane
Affinity purification and char ...
Burkholderia cepacia
Arch. Biochem. Biophys.
288
169-176
1991
-
-
-
-
-
-
8
2
-
-
2
1
-
3
-
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1
-
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1
-
1
1
1
-
-
-
1
-
-
3
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3
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8
-
2
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-
2
1
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-
-
1
-
-
1
-
1
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
439012
Liu
Purification and properties of ...
Cupriavidus necator, Cupriavidus necator JMP 134-1
FEBS Lett.
173
314-318
1984
-
-
1
-
-
-
11
4
-
-
3
2
-
27
-
-
1
-
-
-
1
-
13
1
-
-
-
-
2
-
-
3
-
-
-
-
-
1
3
-
-
-
-
11
-
4
-
-
3
2
-
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1
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1
-
13
1
-
-
-
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2
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-
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-
-
-
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-
439011
Beadle
The purification and propertie ...
Acinetobacter sp.
Eur. J. Biochem.
123
323-332
1982
-
-
-
-
-
4
10
3
-
-
2
1
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3
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-
1
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1
1
7
1
1
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1
1
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3
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-
-
3
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-
4
-
10
-
3
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2
1
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-
1
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-
1
1
7
1
1
-
-
1
1
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