BRENDA - Enzyme Database show
show all sequences of 1.14.13.2

A functional 4-hydroxybenzoate degradation pathway in the phytopathogen Xanthomonas campestris is required for full pathogenicity

Wang, J.-Y.; Zhou, L.; Chen, B.; Sun, S.; Zhang, W.; Li, M.; Tang, H.; Jiang, B.-L.; Tang, J.L.; He, Y.-W.; Sci. Rep. 5, 18456 (2015)
No PubMed abstract available

Data extracted from this reference:

Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4-hydroxybenzoate + NADPH + H+ + O2
Xanthomonas campestris
-
protocatechuate + NADP+ + H2O
-
-
?
4-hydroxybenzoate + NADPH + H+ + O2
Xanthomonas campestris ATCC 33913
-
protocatechuate + NADP+ + H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Xanthomonas campestris
-
Xanthomonas campestris pv. campestris
-
Xanthomonas campestris ATCC 33913
-
Xanthomonas campestris pv. campestris
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-hydroxybenzoate + NADPH + H+ + O2
-
743802
Xanthomonas campestris
protocatechuate + NADP+ + H2O
-
-
-
?
4-hydroxybenzoate + NADPH + H+ + O2
-
743802
Xanthomonas campestris ATCC 33913
protocatechuate + NADP+ + H2O
-
-
-
?
additional information
no activity with 2-hydroxybenzoate and 3-hydroxybenzoate
743802
Xanthomonas campestris
?
-
-
-
-
additional information
no activity with 2-hydroxybenzoate and 3-hydroxybenzoate
743802
Xanthomonas campestris ATCC 33913
?
-
-
-
-
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
-
Xanthomonas campestris
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
-
Xanthomonas campestris
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4-hydroxybenzoate + NADPH + H+ + O2
Xanthomonas campestris
-
protocatechuate + NADP+ + H2O
-
-
?
4-hydroxybenzoate + NADPH + H+ + O2
Xanthomonas campestris ATCC 33913
-
protocatechuate + NADP+ + H2O
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-hydroxybenzoate + NADPH + H+ + O2
-
743802
Xanthomonas campestris
protocatechuate + NADP+ + H2O
-
-
-
?
4-hydroxybenzoate + NADPH + H+ + O2
-
743802
Xanthomonas campestris ATCC 33913
protocatechuate + NADP+ + H2O
-
-
-
?
additional information
no activity with 2-hydroxybenzoate and 3-hydroxybenzoate
743802
Xanthomonas campestris
?
-
-
-
-
additional information
no activity with 2-hydroxybenzoate and 3-hydroxybenzoate
743802
Xanthomonas campestris ATCC 33913
?
-
-
-
-
Expression
Organism
Commentary
Expression
Xanthomonas campestris
enzyme expression is induced by 4-hydroxybenzoate via the transcriptional activator PobR. Addition of radish and cabbage hydrolysates to culture significantly induces the expression of the enzyme via PobR
up
Expression (protein specific)
Organism
Commentary
Expression
Xanthomonas campestris
enzyme expression is induced by 4-hydroxybenzoate via the transcriptional activator PobR. Addition of radish and cabbage hydrolysates to culture significantly induces the expression of the enzyme via PobR
up
Other publictions for EC 1.14.13.2
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
742143
Chen
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2571-2580
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742469
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Microbial community structure ...
Haloferax volcanii, Haloferax volcanii DS2
Extremophiles
20
311-321
2016
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11
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743802
Wang
-
A functional 4-hydroxybenzoat ...
Xanthomonas campestris, Xanthomonas campestris ATCC 33913
Sci. Rep.
5
18456
2015
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4
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1
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1
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726322
Romero-Silva
Genomic and functional analyse ...
Paraburkholderia xenovorans
PLoS ONE
8
e56038
2013
-
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3
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1
1
-
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724101
Chen
Biodegradation of 4-hydroxyben ...
Diaporthe liquidambaris, Diaporthe liquidambaris B3
Appl. Soil Ecol.
51
102-110
2011
-
1
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2
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2
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724858
Donoso
Strict and direct transcriptio ...
Cupriavidus necator, Cupriavidus necator JMP 134-1
Environ. Microbiol.
13
1590-1600
2011
-
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26
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1
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1
-
-
704290
Zimmermann
Role of Acinetobacter baylyi C ...
Acinetobacter baylyi, Acinetobacter baylyi ADPU54
J. Bacteriol.
191
2834-2842
2009
1
-
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4
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684641
Huang
Genetic and biochemical charac ...
Corynebacterium glutamicum
Appl. Microbiol. Biotechnol.
78
75-83
2008
2
-
-
-
4
-
6
10
-
1
2
1
-
2
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1
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4
1
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10
1
1
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2
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2
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2
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4
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6
-
10
-
1
2
1
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1
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-
4
1
-
-
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10
1
1
-
-
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-
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-
684919
Chang
Examination and expansion of t ...
Comamonas testosteroni, Comamonas testosteroni GZ39
Biochem. Biophys. Res. Commun.
371
149-153
2008
-
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1
-
14
-
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3
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18
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1
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1
1
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14
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18
-
-
-
-
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686087
Kudryashova
Monomer formation and function ...
Pseudomonas fluorescens
Chembiochem
9
413-419
2008
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4
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1
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1
2
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4
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686803
Kim
Molecular cloning and function ...
Chromohalobacter sp., Chromohalobacter sp. HS-2
FEMS Microbiol. Lett.
280
235-241
2008
-
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1
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2
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684215
Kwon
Expression, purification, crys ...
Corynebacterium glutamicum
Acta Crystallogr. Sect. F
F63
944-946
2007
-
-
1
1
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2
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1
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686783
Deveryshetty
Metabolism of 2-, 3- and 4-hyd ...
Alcaligenes sp., Pseudomonas sp.
FEMS Microbiol. Lett.
268
59-66
2007
-
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687939
Van Den Berg
-
Time-resolved fluorescence ana ...
Pseudomonas fluorescens
J. Chem. Sci.
119
123-133
2007
-
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2
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1
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690069
Cui
-
Development of an L-glutamate ...
Pseudomonas sp.
Sens. Actuators B Chem.
B127
358-361
2007
-
1
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742538
Ceveryshetty
Metabolism of 2-, 3- and 4-hy ...
Alcaligenes sp. PPH, Pseudomonas sp. PPD
FEMS Microbiol. Lett.
268
59-66
2007
-
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742966
Van Den Berg
-
Time-resolved fluorescence an ...
Pseudomonas fluorescens
J. Chem. Sci.
119
123-133
2007
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1
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2
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743852
Cui
-
Development of an L-glutamate ...
Pseudomonas sp.
Sens. Actuators B Chem.
B127
358-361
2007
-
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661173
Frederick
Kinetics of proton-linked flav ...
Pseudomonas aeruginosa
Biochemistry
44
13304-13314
2005
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1
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658045
Ortiz-Maldonado
Oxygen reactions in p-hydroxyb ...
Pseudomonas aeruginosa
Biochemistry
43
15246-15257
2004
-
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1
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5
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1
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1
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1
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5
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658048
Ortiz-Maldonado
Increased positive electrostat ...
Pseudomonas aeruginosa
Biochemistry
43
1569-1579
2004
-
-
1
-
1
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1
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1
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1
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1
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1
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4
-
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2
1
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1
2
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1
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1
1
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1
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4
-
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657951
Ortiz-Maldonado
Conformational changes combine ...
Pseudomonas aeruginosa
Biochemistry
42
11234-11242
2003
1
-
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1
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657936
Palfey
Role of protein flexibility in ...
Pseudomonas aeruginosa
Biochemistry
41
8438-8446
2002
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1
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1
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1
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2
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660408
Wang
Protein and ligand dynamics in ...
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390036
Jadan
Purification and properties of ...
Rhodococcus opacus, Rhodococcus rhodnii, Rhodococcus rhodnii 135, Rhodococcus rhodochrous, Rhodococcus rhodochrous 172, Rhodococcus sp., Rhodococcus sp. 400
Biochemistry (Moscow)
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2001
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390056
Ortiz-Maldonado
Synergistic interactions of mu ...
Pseudomonas aeruginosa
Biochemistry
40
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2001
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681785
Bertani
Regulation of the p-hydroxyben ...
Pseudomonas putida, Pseudomonas putida WCS358
Microbiology
147
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2001
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1
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390043
Abe
Antioxidative galloyl esters a ...
Pseudomonas aeruginosa, Pseudomonas fluorescens
FEBS Lett.
483
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2000
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1
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390037
Moran
Mechanistic insights into p-hy ...
Pseudomonas aeruginosa
Biochemistry
38
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1999
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390055
Ortiz-Maldonado
Structure-function correlation ...
Pseudomonas aeruginosa
Biochemistry
38
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1999
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390044
Eppink
Lys42 and Ser42 variants of p- ...
Pseudomonas fluorescens
Eur. J. Biochem.
253
194-201
1998
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1
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2
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4
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390046
Eppink
Interdomain binding of NADPH i ...
Pseudomonas fluorescens
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1998
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1
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13
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10
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1
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1
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390048
Chen
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Crystallization and further ch ...
Comamonas testosteroni
Res. Commun. Biochem. Cell Mol. Biol.
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1998
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1
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390047
Chen
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Purification and characterizat ...
Acinetobacter sp., Comamonas testosteroni, Comamonas testosteroni Kh 122-3S, Pseudomonas sp.
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8
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390045
Seibold
4-Hydroxybenzoate hydroxylase ...
Pseudomonas sp., Pseudomonas sp. CBS3
Eur. J. Biochem.
239
469-478
1996
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1
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1
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1
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7
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Fernandez
Purification and characterizat ...
Acinetobacter calcoaceticus
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1995
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1
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7
4
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1
1
3
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1
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390039
Suarez
Purification and characterizat ...
Klebsiella pneumoniae
Arch. Microbiol.
164
70-77
1995
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1
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390054
Moran
Plasmid mutagenesis by PCR for ...
Pseudomonas aeruginosa
Protein Expr. Purif.
6
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1995
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390041
Salituro
Multisubstrate inhibition of 4 ...
Pseudomonas fluorescens
J. Med. Chem.
37
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1994
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390042
van Berkel
Crystal structure of p-hydroxy ...
Pseudomonas fluorescens
Protein Sci.
3
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1994
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390049
Schreuder
Crystal structures of wild-typ ...
Pseudomonas fluorescens
Biochemistry
33
10161-10170
1994
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1
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1
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390050
Lah
Crystal structures of mutant P ...
Pseudomonas aeruginosa
Biochemistry
33
1555-1564
1994
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390051
Suemori
-
Comparison of chemical inactiv ...
Rhodococcus erythropolis
Seimei Kogaku Kogyo Gijutsu Kenkyusho Kenkyu Hokoku
2
27-30
1994
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390053
Palfey
Changes in the catalytic prope ...
Pseudomonas aeruginosa
Biochemistry
33
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1994
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Sterjiades
-
Properties of NADH/NADPH-depen ...
Moraxella sp., Moraxella sp. GU2
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Whited
Separation and partial charact ...
Pseudomonas mendocina, Pseudomonas mendocina KR1
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Entsch
Catalytic function of tyrosine ...
Pseudomonas aeruginosa
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Entsch
Hydroxybenzoate hydroxylase ...
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van Berkel
The temperature and pH depende ...
Pseudomonas fluorescens
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1989
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Entsch
Purification, properties, and ...
Pseudomonas aeruginosa
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Schreuder
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van Berkel
The elucidation of the microhe ...
Pseudomonas fluorescens
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van Berkel
Chemical modification of sulfh ...
Pseudomonas fluorescens
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145
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1984
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1
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2
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1
-
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390024
Shoun
Inhibition of p-hydroxybenzoat ...
Delftia acidovorans
J. Biochem.
93
169-176
1983
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-
-
-
-
-
16
1
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2
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1
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1
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1
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16
-
1
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1
-
-
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-
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-
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390031
Shoun
An essential arginine residue ...
Delftia acidovorans
J. Biol. Chem.
255
9319-9324
1980
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-
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1
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1
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1
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1
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1
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1
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-
-
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1
-
-
-
-
-
-
-
-
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-
390032
Mueller
A study of p-hydroxybenzoate h ...
Pseudomonas fluorescens
Eur. J. Biochem.
101
235-244
1979
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-
-
-
-
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2
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2
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1
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1
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1
1
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1
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1
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2
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1
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-
1
-
1
1
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-
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-
390023
Husain
p-Hydroxybenzoate hydroxylase ...
Delftia acidovorans, Pseudomonas fluorescens, Pseudomonas putida, Pseudomonas putida A 3.12, Pseudomonas putida M-6
Methods Enzymol.
53
543-558
1978
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1
-
2
5
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1
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6
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1
1
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1
1
9
-
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1
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4
-
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4
1
-
2
-
5
-
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1
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1
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1
1
9
-
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1
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-
390029
Entsch
Flavin-oxygen derivatives invo ...
Pseudomonas fluorescens
J. Biol. Chem.
251
2550-2563
1976
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2
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2
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1
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1
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2
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-
390025
Drenth
Crystallization and preliminar ...
Pseudomonas fluorescens
J. Biol. Chem.
250
5268-5269
1975
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1
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1
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2
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2
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1
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1
1
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1
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2
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390022
Steennis
On the interaction of para-hyd ...
Pseudomonas fluorescens
FEBS Lett.
36
177-180
1973
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5
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1
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2
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2
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1
5
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1
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5
5
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1
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2
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390018
Howell
Purification and properties of ...
Pseudomonas fluorescens
J. Biol. Chem.
247
4340-4350
1972
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1
-
1
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1
1
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2
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1
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1
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3
-
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1
2
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2
1
-
1
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1
1
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-
1
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1
-
3
-
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1
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390019
Spector
Studies on the effector specif ...
Pseudomonas fluorescens
J. Biol. Chem.
247
4679-4687
1972
3
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9
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2
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2
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1
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3
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1
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9
-
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2
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390020
Spector
p-Hydroxybenzoate hydroxylase ...
Pseudomonas fluorescens
J. Biol. Chem.
247
5632-5636
1972
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3
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2
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2
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1
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1
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3
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2
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390021
Hosokawa
Crystallization and properties ...
Pseudomonas fluorescens
J. Biol. Chem.
241
2453-2460
1966
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1
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9
2
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2
1
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2
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7
-
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1
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2
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2
1
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9
-
2
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2
1
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7
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1
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