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Literature summary for 1.14.13.2 extracted from

  • Ceveryshetty, J.; Suvekbala, V.; Varadamshetty, G.; Phale, P.S.
    Metabolism of 2-, 3- and 4-hydroxybenzoates by soil isolates Alcaligenes sp. strain PPH and Pseudomonas sp. strain PPD (2007), FEMS Microbiol. Lett., 268, 59-66 .
    View publication on PubMed

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-hydroxybenzoate + NADPH + H+ + O2 Alcaligenes sp. PPH
-
protocatechuate + NADP+ + H2O
-
?
4-hydroxybenzoate + NADPH + H+ + O2 Pseudomonas sp. PPD
-
protocatechuate + NADP+ + H2O
-
?

Organism

Organism UniProt Comment Textmining
Alcaligenes sp. PPH
-
-
-
Pseudomonas sp. PPD
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.016
-
at pH 7.5, temperature not specified in the publication Alcaligenes sp. PPH
0.281
-
at pH 7.5, temperature not specified in the publication Pseudomonas sp. PPD

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-hydroxybenzoate + NADPH + H+ + O2
-
Alcaligenes sp. PPH protocatechuate + NADP+ + H2O
-
?
4-hydroxybenzoate + NADPH + H+ + O2
-
Pseudomonas sp. PPD protocatechuate + NADP+ + H2O
-
?
additional information the enzyme also shows low activities with 2- and 3-hydroxybenzoate as well as D-glucose Alcaligenes sp. PPH ?
-
?
additional information the enzyme also shows low activities with 2- and 3-hydroxybenzoate as well as D-glucose Pseudomonas sp. PPD ?
-
?

Synonyms

Synonyms Comment Organism
4-HBA 3-hydroxylase
-
Alcaligenes sp. PPH
4-HBA 3-hydroxylase
-
Pseudomonas sp. PPD

Cofactor

Cofactor Comment Organism Structure
FAD
-
Alcaligenes sp. PPH
FAD
-
Pseudomonas sp. PPD
NADPH
-
Alcaligenes sp. PPH
NADPH
-
Pseudomonas sp. PPD