Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-ornithine + NADPH + H+ + O2 | Aspergillus fumigatus | the enzyme catalyzes the hydroxylation of ornithine in the biosynthesis of hydroxamate siderophores that are essential for virulence | N5-hydroxy-L-ornithine + NADP+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus fumigatus | Q5SE95 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-ornithine + NADPH + H+ + O2 | the enzyme catalyzes the hydroxylation of ornithine in the biosynthesis of hydroxamate siderophores that are essential for virulence | Aspergillus fumigatus | N5-hydroxy-L-ornithine + NADP+ + H2O | - |
? | |
L-ornithine + NADPH + H+ + O2 | in the reductive half-reaction, the oxidized FAD bound to the enzyme, is reduced by NADPH. In the oxidative half-reaction, the reduced cofactor reacts with molecular oxygen to form a C4a-hydroperoxyflavin intermediate, which transfers an oxygen atom to ornithine | Aspergillus fumigatus | N5-hydroxy-L-ornithine + NADP+ + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SidA | - |
Aspergillus fumigatus |
siderophore A | - |
Aspergillus fumigatus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | monitoring the reductive and oxidative half-reactions of a flavin-dependent monooxygenase using stopped-flow spectrophotometry | Aspergillus fumigatus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | FAD-containing enzyme. In the reductive half-reaction, the oxidized FAD bound to the enzyme, is reduced by NADPH. In the oxidative half-reaction, the reduced cofactor reacts with molecular oxygen to form a C4a-hydroperoxyflavin intermediate, which transfers an oxygen atom to ornithine | Aspergillus fumigatus |