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Literature summary for 1.14.13.195 extracted from

  • Shirey, C.; Badieyan, S.; Sobrado, P.
    Role of Ser-257 in the sliding mechanism of NADP(H) in the reaction catalyzed by the Aspergillus fumigatus flavin-dependent ornithine N5-monooxygenase SidA (2013), J. Biol. Chem., 288, 32440-32448.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.13.195

Cloned(Commentary)

Cloned (Comment) Organism
-
 Aspergillus fumigatus

Protein Variants

Protein Variants Comment Organism
S257A the interaction between residue Ser257 and NADP(H) is essential for stabilization of the C4a-hydroperoxyflavin intermediate. Ser257 may function as a pivot point, allowing the nicotinamide of NADP+ to slide into position for stabilization of the C4a-hydroperoxyflavin Aspergillus fumigatus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.014
-
 NADPH mutant S257A, O2 consumption assay, pH 7.5, 25°C Aspergillus fumigatus
0.017
-
 NADPH wild-type, O2 consumption assay, pH 7.5, 25°C Aspergillus fumigatus
0.34
-
 NADPH mutant S257A, hydroxylamine detection assay, pH 7.5, 25°C Aspergillus fumigatus
0.8
-
 NADPH wild-type, hydroxylamine detection assay, pH 7.5, 25°C Aspergillus fumigatus

Organism

Organism UniProt Comment Textmining
Aspergillus fumigatus Q5SE95
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-ornithine + NADPH + H+ + O2
-
 Aspergillus fumigatus N5-hydroxy-L-ornithine + NADP+ + H2O
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.12
-
 NADPH mutant S257A, hydroxylamine detection assay, pH 7.5, 25°C Aspergillus fumigatus
0.45
-
 NADPH wild-type, hydroxylamine detection assay, pH 7.5, 25°C Aspergillus fumigatus
0.6
-
 NADPH wild-type, O2 consumption assay, pH 7.5, 25°C Aspergillus fumigatus
0.85
-
 NADPH mutant S257A, O2 consumption assay, pH 7.5, 25°C Aspergillus fumigatus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.34
-
 NADPH mutant S257A, hydroxylamine detection assay, pH 7.5, 25°C Aspergillus fumigatus
0.54
-
 NADPH wild-type, hydroxylamine detection assay, pH 7.5, 25°C Aspergillus fumigatus
14
-
 NADPH wild-type, O2 consumption assay, pH 7.5, 25°C Aspergillus fumigatus
40
-
 NADPH mutant S257A, O2 consumption assay, pH 7.5, 25°C Aspergillus fumigatus