BRENDA - Enzyme Database show
show all sequences of 1.14.13.195

Membrane-association determinants of the omega-amino acid monooxygenase PvdA, a pyoverdine biosynthetic enzyme from Pseudomonas aeruginosa

Imperi, F.; Putignani, L.; Tiburzi, F.; Ambrosi, C.; Cipollone, R.; Ascenzi, P.; Visca, P.; Microbiology 154, 2804-2813 (2008)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression of PvdA-PhoA fusion protein mutants in Escherichia coli strain LMG194
Pseudomonas aeruginosa
Engineering
Amino acid exchange
Commentary
Organism
additional information
construction of PvdA-PhoA fusion protein mutantsa nd of a pvdA deletion mutant strain
Pseudomonas aeruginosa
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
inner membrane
the enzyme anchors in the inner membrane with its N-terminus, while the bulk of the enzyme is located in the cytosol, the transmembrane region overlaps the FAd binding site, membrane-association determinants of PvdA and analysis of membrane topology, overview
Pseudomonas aeruginosa
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas aeruginosa
-
gene pvdA
-
Posttranslational Modification
Posttranslational Modification
Commentary
Organism
flavoprotein
-
Pseudomonas aeruginosa
Subunits
Subunits
Commentary
Organism
More
PvdA secondary structure and transmembrane region, overview
Pseudomonas aeruginosa
Cofactor
Cofactor
Commentary
Organism
Structure
FAD
binding motif, overview
Pseudomonas aeruginosa
NADPH
binding motif, overview
Pseudomonas aeruginosa
Cloned(Commentary) (protein specific)
Commentary
Organism
expression of PvdA-PhoA fusion protein mutants in Escherichia coli strain LMG194
Pseudomonas aeruginosa
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
FAD
binding motif, overview
Pseudomonas aeruginosa
NADPH
binding motif, overview
Pseudomonas aeruginosa
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
additional information
construction of PvdA-PhoA fusion protein mutantsa nd of a pvdA deletion mutant strain
Pseudomonas aeruginosa
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
inner membrane
the enzyme anchors in the inner membrane with its N-terminus, while the bulk of the enzyme is located in the cytosol, the transmembrane region overlaps the FAd binding site, membrane-association determinants of PvdA and analysis of membrane topology, overview
Pseudomonas aeruginosa
-
-
Posttranslational Modification (protein specific)
Posttranslational Modification
Commentary
Organism
flavoprotein
-
Pseudomonas aeruginosa
Subunits (protein specific)
Subunits
Commentary
Organism
More
PvdA secondary structure and transmembrane region, overview
Pseudomonas aeruginosa
Other publictions for EC 1.14.13.195
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
743137
Badieyan
Mechanism of N-hydroxylation ...
Aspergillus fumigatus, Aspergillus fumigatus Af293
J. Org. Chem.
80
2139-2147
2015
-
-
-
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
744163
Robinson
Contribution to catalysis of ...
Aspergillus fumigatus
Arch. Biochem. Biophys.
585
25-31
2015
-
-
1
1
4
-
-
12
-
-
-
1
-
2
-
-
1
1
-
-
-
-
1
-
1
-
-
8
1
-
-
1
-
-
-
-
-
1
1
1
4
-
-
-
-
12
-
-
-
1
-
-
-
1
-
-
-
-
1
-
1
-
-
8
1
-
-
-
-
1
1
-
8
8
725540
Shirey
Role of Ser-257 in the sliding ...
Aspergillus fumigatus
J. Biol. Chem.
288
32440-32448
2013
-
-
1
-
1
-
-
4
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
4
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
4
-
-
-
-
-
-
-
-
4
4
724451
Romero
Dual role of NADP(H) in the re ...
Aspergillus fumigatus
Biochim. Biophys. Acta
1824
850-857
2012
-
-
-
-
-
-
-
2
-
-
-
-
-
2
-
-
-
-
-
-
-
-
3
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
2
-
-
-
-
-
1
1
-
2
2
724860
Guillon
High cellular organization of ...
Pseudomonas aeruginosa
Environ. Microbiol.
14
1982-1994
2012
-
-
-
-
-
-
-
-
1
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
714263
Robbel
Consecutive enzymatic modifica ...
Saccharopolyspora erythraea
Biochemistry
50
6073-6080
2011
-
-
1
-
-
-
-
1
-
-
-
1
-
1
-
-
1
-
-
-
-
-
2
-
1
-
-
1
1
-
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
1
-
-
-
1
-
-
-
1
-
-
-
-
2
-
1
-
-
1
1
-
-
-
-
-
-
-
1
1
725195
Frederick
Regulated O2 activation in fla ...
Aspergillus fumigatus
J. Am. Chem. Soc.
133
12338-12341
2011
1
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
725440
Olucha
Two structures of an N-hydroxy ...
Pseudomonas aeruginosa
J. Biol. Chem.
286
31789-31798
2011
-
-
-
1
-
-
1
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
1
-
-
-
-
-
-
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
702303
Meneely
Kinetic mechanism of ornithine ...
Pseudomonas aeruginosa
Biochemistry
48
4371-4376
2009
-
-
-
-
-
-
-
1
-
-
-
1
-
2
-
1
-
1
-
-
-
-
3
-
1
-
-
-
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
1
-
-
-
1
-
-
1
-
-
-
-
-
3
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
705555
Imperi
Membrane-association determina ...
Pseudomonas aeruginosa
Microbiology
154
2804-2813
2008
-
-
1
-
1
-
-
-
1
-
-
-
-
4
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
2
-
-
-
-
-
1
2
-
1
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
706031
Pohlmann
delta-Amino group hydroxylatio ...
Streptomyces coelicolor, Streptomyces coelicolor A3(2)
Org. Biomol. Chem.
6
1843-1848
2008
-
-
1
-
-
-
-
1
-
-
-
2
-
42
-
1
1
-
-
-
-
-
6
-
1
-
-
-
1
1
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
1
-
-
-
2
-
-
1
1
-
-
-
-
6
-
1
-
-
-
1
1
-
-
-
1
1
-
-
-
702210
Meneely
Biochemical characterization o ...
Pseudomonas aeruginosa
Biochemistry
46
11930-11937
2007
-
-
-
-
-
-
3
1
1
-
-
3
-
5
-
1
-
-
-
-
2
-
5
1
-
-
-
-
1
1
-
3
-
-
-
-
-
-
3
-
-
-
-
3
-
1
1
-
-
3
-
-
1
-
-
-
2
-
5
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
704269
Ge
Heterologous expression, purif ...
Pseudomonas aeruginosa
J. Bacteriol.
188
7205-7210
2006
2
-
1
-
-
-
3
4
-
-
2
1
-
1
-
1
1
-
-
-
1
-
3
1
1
-
-
-
1
1
-
3
4
-
-
2
-
1
3
-
-
-
-
3
4
4
-
-
2
1
-
-
1
1
-
-
1
-
3
1
1
-
-
-
1
1
-
-
-
1
1
-
-
-
702009
Putignani
Expression of L-ornithine Ndel ...
Burkholderia cepacia, Pseudomonas aeruginosa, Pseudomonas fluorescens, Pseudomonas putida, Pseudomonas syringae, Ralstonia solanacearum
Biochem. Biophys. Res. Commun.
313
245-257
2004
-
-
6
-
1
-
-
-
-
-
-
6
-
15
-
6
1
-
-
-
-
-
12
1
-
-
-
-
-
-
-
12
-
1
-
-
-
6
12
-
1
-
-
-
-
-
-
-
-
6
-
-
6
1
-
-
-
-
12
1
-
-
-
-
-
-
-
1
5
-
-
5
-
-
704262
Ambrosi
Pseudobactin biogenesis, in th ...
Pseudomonas sp., Pseudomonas sp. B10
J. Bacteriol.
182
6233-6238
2000
-
-
1
-
1
-
-
-
-
1
-
2
-
2
-
1
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
2
-
1
-
-
-
-
-
-
1
-
2
-
-
1
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-