BRENDA - Enzyme Database show
show all sequences of 1.14.13.195

Pseudobactin biogenesis, in the plant growth-promoting rhizobacterium Pseudomonas strain B10: Identification and functional analysis of the L-ornithine N5-oxygenase (psbA) gene

Ambrosi, C.; Leoni, L.; Putignani, L.; Orsi, N.; Visca, P.; J. Bacteriol. 182, 6233-6238 (2000)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene psbA, DNA and amino acid sequence determination and analysis, monocistronic gene
Pseudomonas sp.
Engineering
Amino acid exchange
Commentary
Organism
additional information
a PsbA-defective mutant B10CA1 is fully complemented by addition of L-ornithine N5-oxide, the mutant strain shows impaired synthesis of fluorescent pigment and hydroxamate nitrogen in iron-poor medium, as well as defective biosynthesis of both forms of pseudobactin and salicylate-based siderophores, Fe3+ transfer is impaired, phenotype, overview
Pseudomonas sp.
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Iron
the psbA expression is negatively controlled by iron
Pseudomonas sp.
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-ornithine + NADPH + H+ + O2
Pseudomonas sp.
-
L-ornithine N5-oxide + NADP+ + H2O
-
-
?
L-ornithine + NADPH + H+ + O2
Pseudomonas sp. B10
-
L-ornithine N5-oxide + NADP+ + H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas sp.
Q9F8X0
gene psbA
-
Pseudomonas sp. B10
Q9F8X0
gene psbA
-
Posttranslational Modification
Posttranslational Modification
Commentary
Organism
flavoprotein
-
Pseudomonas sp.
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-ornithine + NADPH + H+ + O2
-
704262
Pseudomonas sp.
L-ornithine N5-oxide + NADP+ + H2O
-
-
-
?
L-ornithine + NADPH + H+ + O2
the enzyme contains the FATGY signature in the putative substrate binding pocket
704262
Pseudomonas sp.
L-ornithine N5-oxide + NADP+ + H2O
-
-
-
?
L-ornithine + NADPH + H+ + O2
-
704262
Pseudomonas sp. B10
L-ornithine N5-oxide + NADP+ + H2O
-
-
-
?
L-ornithine + NADPH + H+ + O2
the enzyme contains the FATGY signature in the putative substrate binding pocket
704262
Pseudomonas sp. B10
L-ornithine N5-oxide + NADP+ + H2O
-
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
FAD
-
Pseudomonas sp.
NADPH
-
Pseudomonas sp.
Cloned(Commentary) (protein specific)
Commentary
Organism
gene psbA, DNA and amino acid sequence determination and analysis, monocistronic gene
Pseudomonas sp.
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
FAD
-
Pseudomonas sp.
NADPH
-
Pseudomonas sp.
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
additional information
a PsbA-defective mutant B10CA1 is fully complemented by addition of L-ornithine N5-oxide, the mutant strain shows impaired synthesis of fluorescent pigment and hydroxamate nitrogen in iron-poor medium, as well as defective biosynthesis of both forms of pseudobactin and salicylate-based siderophores, Fe3+ transfer is impaired, phenotype, overview
Pseudomonas sp.
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Iron
the psbA expression is negatively controlled by iron
Pseudomonas sp.
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-ornithine + NADPH + H+ + O2
Pseudomonas sp.
-
L-ornithine N5-oxide + NADP+ + H2O
-
-
?
L-ornithine + NADPH + H+ + O2
Pseudomonas sp. B10
-
L-ornithine N5-oxide + NADP+ + H2O
-
-
?
Posttranslational Modification (protein specific)
Posttranslational Modification
Commentary
Organism
flavoprotein
-
Pseudomonas sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-ornithine + NADPH + H+ + O2
-
704262
Pseudomonas sp.
L-ornithine N5-oxide + NADP+ + H2O
-
-
-
?
L-ornithine + NADPH + H+ + O2
the enzyme contains the FATGY signature in the putative substrate binding pocket
704262
Pseudomonas sp.
L-ornithine N5-oxide + NADP+ + H2O
-
-
-
?
L-ornithine + NADPH + H+ + O2
-
704262
Pseudomonas sp. B10
L-ornithine N5-oxide + NADP+ + H2O
-
-
-
?
L-ornithine + NADPH + H+ + O2
the enzyme contains the FATGY signature in the putative substrate binding pocket
704262
Pseudomonas sp. B10
L-ornithine N5-oxide + NADP+ + H2O
-
-
-
?
Expression
Organism
Commentary
Expression
Pseudomonas sp.
the psbA expression is negatively controlled by iron
down
Expression (protein specific)
Organism
Commentary
Expression
Pseudomonas sp.
the psbA expression is negatively controlled by iron
down
Other publictions for EC 1.14.13.195
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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Badieyan
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744163
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1
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12
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1
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2
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1
1
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1
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1
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8
1
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1
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1
1
1
4
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12
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1
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1
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1
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1
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8
1
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1
1
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8
8
725540
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1
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4
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4
4
724451
Romero
Dual role of NADP(H) in the re ...
Aspergillus fumigatus
Biochim. Biophys. Acta
1824
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2012
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2
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2
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3
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2
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1
1
-
2
2
724860
Guillon
High cellular organization of ...
Pseudomonas aeruginosa
Environ. Microbiol.
14
1982-1994
2012
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1
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1
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1
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1
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-
714263
Robbel
Consecutive enzymatic modifica ...
Saccharopolyspora erythraea
Biochemistry
50
6073-6080
2011
-
-
1
-
-
-
-
1
-
-
-
1
-
1
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1
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2
-
1
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1
1
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2
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1
2
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1
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1
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-
2
-
1
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1
1
-
-
-
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-
-
1
1
725195
Frederick
Regulated O2 activation in fla ...
Aspergillus fumigatus
J. Am. Chem. Soc.
133
12338-12341
2011
1
-
-
-
-
-
-
-
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2
-
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1
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1
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1
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1
-
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1
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-
-
-
-
725440
Olucha
Two structures of an N-hydroxy ...
Pseudomonas aeruginosa
J. Biol. Chem.
286
31789-31798
2011
-
-
-
1
-
-
1
-
-
-
-
-
-
2
-
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1
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1
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1
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1
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1
1
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1
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1
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1
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1
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-
702303
Meneely
Kinetic mechanism of ornithine ...
Pseudomonas aeruginosa
Biochemistry
48
4371-4376
2009
-
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-
-
-
-
-
1
-
-
-
1
-
2
-
1
-
1
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3
-
1
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1
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2
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2
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1
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1
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-
1
-
-
-
-
-
3
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
705555
Imperi
Membrane-association determina ...
Pseudomonas aeruginosa
Microbiology
154
2804-2813
2008
-
-
1
-
1
-
-
-
1
-
-
-
-
4
-
1
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-
-
-
-
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-
1
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2
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1
2
-
1
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1
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1
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1
-
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-
-
706031
Pohlmann
delta-Amino group hydroxylatio ...
Streptomyces coelicolor, Streptomyces coelicolor A3(2)
Org. Biomol. Chem.
6
1843-1848
2008
-
-
1
-
-
-
-
1
-
-
-
2
-
42
-
1
1
-
-
-
-
-
6
-
1
-
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-
1
1
-
2
-
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1
2
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1
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-
2
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1
1
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-
-
6
-
1
-
-
-
1
1
-
-
-
1
1
-
-
-
702210
Meneely
Biochemical characterization o ...
Pseudomonas aeruginosa
Biochemistry
46
11930-11937
2007
-
-
-
-
-
-
3
1
1
-
-
3
-
5
-
1
-
-
-
-
2
-
5
1
-
-
-
-
1
1
-
3
-
-
-
-
-
-
3
-
-
-
-
3
-
1
1
-
-
3
-
-
1
-
-
-
2
-
5
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
704269
Ge
Heterologous expression, purif ...
Pseudomonas aeruginosa
J. Bacteriol.
188
7205-7210
2006
2
-
1
-
-
-
3
4
-
-
2
1
-
1
-
1
1
-
-
-
1
-
3
1
1
-
-
-
1
1
-
3
4
-
-
2
-
1
3
-
-
-
-
3
4
4
-
-
2
1
-
-
1
1
-
-
1
-
3
1
1
-
-
-
1
1
-
-
-
1
1
-
-
-
702009
Putignani
Expression of L-ornithine Ndel ...
Burkholderia cepacia, Pseudomonas aeruginosa, Pseudomonas fluorescens, Pseudomonas putida, Pseudomonas syringae, Ralstonia solanacearum
Biochem. Biophys. Res. Commun.
313
245-257
2004
-
-
6
-
1
-
-
-
-
-
-
6
-
15
-
6
1
-
-
-
-
-
12
1
-
-
-
-
-
-
-
12
-
1
-
-
-
6
12
-
1
-
-
-
-
-
-
-
-
6
-
-
6
1
-
-
-
-
12
1
-
-
-
-
-
-
-
1
5
-
-
5
-
-
704262
Ambrosi
Pseudobactin biogenesis, in th ...
Pseudomonas sp., Pseudomonas sp. B10
J. Bacteriol.
182
6233-6238
2000
-
-
1
-
1
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1
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2
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2
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1
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2
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1
2
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1
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4
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