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show all sequences of 1.14.13.190

RNA interference targeting CYP76AH1 in hairy roots of Salvia miltiorrhiza reveals its key role in the biosynthetic pathway of tanshinones

Ma, Y.; Ma, X.H.; Meng, F.Y.; Zhan, Z.L.; Guo, J.; Huang, L.Q.; Biochem. Biophys. Res. Commun. 477, 155-160 (2016)

Data extracted from this reference:

Engineering
Amino acid exchange
Commentary
Organism
additional information
RNAi-mediated silencing of CYP76AH1 in hairy roots, construction of a CYP76AH1 RNAi system for hairy roots using transformation via Agrobacterium rhizogenes strain C58C1. Metabolic analysis of RNAi-AH1 hairy root lines shows a significantly increased accumulation of miltiradiene and decreased concentration of ferruginol compared to the control lines. The content of tanshinones decreases significantly after silencing of CYP76AH1
Salvia miltiorrhiza
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
abieta-8,11,13-triene + NADPH + H+ + O2
Salvia miltiorrhiza
-
ferruginol + NADP+ + H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Salvia miltiorrhiza
S4UX02
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
hairy root
-
Salvia miltiorrhiza
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
abieta-8,11,13-triene + NADPH + H+ + O2
-
744235
Salvia miltiorrhiza
ferruginol + NADP+ + H2O
-
-
-
?
abieta-8,11,13-triene + NADPH + H+ + O2
i.e. miltiradiene
744235
Salvia miltiorrhiza
ferruginol + NADP+ + H2O
-
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
cytochrome P-450
-
Salvia miltiorrhiza
NADPH
-
Salvia miltiorrhiza
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
cytochrome P-450
-
Salvia miltiorrhiza
NADPH
-
Salvia miltiorrhiza
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
additional information
RNAi-mediated silencing of CYP76AH1 in hairy roots, construction of a CYP76AH1 RNAi system for hairy roots using transformation via Agrobacterium rhizogenes strain C58C1. Metabolic analysis of RNAi-AH1 hairy root lines shows a significantly increased accumulation of miltiradiene and decreased concentration of ferruginol compared to the control lines. The content of tanshinones decreases significantly after silencing of CYP76AH1
Salvia miltiorrhiza
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
abieta-8,11,13-triene + NADPH + H+ + O2
Salvia miltiorrhiza
-
ferruginol + NADP+ + H2O
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
hairy root
-
Salvia miltiorrhiza
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
abieta-8,11,13-triene + NADPH + H+ + O2
-
744235
Salvia miltiorrhiza
ferruginol + NADP+ + H2O
-
-
-
?
abieta-8,11,13-triene + NADPH + H+ + O2
i.e. miltiradiene
744235
Salvia miltiorrhiza
ferruginol + NADP+ + H2O
-
-
-
?
General Information
General Information
Commentary
Organism
metabolism
the enzyme catalyzes the first committed step in the biosynthesis of tanshinones, pathay overview
Salvia miltiorrhiza
physiological function
CYP76AH1 catalyzes the conversion of the carbon skeleton miltiradiene into the intermediate ferruginol, which is involved in the biosynthesis of tanshinones, the chief bioactive ingredients of Salvia miltiorrhiza
Salvia miltiorrhiza
General Information (protein specific)
General Information
Commentary
Organism
metabolism
the enzyme catalyzes the first committed step in the biosynthesis of tanshinones, pathay overview
Salvia miltiorrhiza
physiological function
CYP76AH1 catalyzes the conversion of the carbon skeleton miltiradiene into the intermediate ferruginol, which is involved in the biosynthesis of tanshinones, the chief bioactive ingredients of Salvia miltiorrhiza
Salvia miltiorrhiza
Other publictions for EC 1.14.13.190
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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7
8855
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1
1
1
2
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4
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1
1
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1
2
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-
744235
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RNA interference targeting CY ...
Salvia miltiorrhiza
Biochem. Biophys. Res. Commun.
477
155-160
2016
-
-
-
-
1
-
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1
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3
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1
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1
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745860
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7
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3
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746312
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Carnosic acid biosynthesis el ...
Salvia pomifera
Proc. Natl. Acad. Sci. USA
113
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1
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2
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730539
Zi
Characterization of CYP76AH4 c ...
Salvia rosmarinus
Org. Biomol. Chem.
11
7650-7652
2013
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1
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1
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1
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3
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1
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1
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1
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1
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