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Literature summary for 1.14.13.19 extracted from

  • Jeffrey, A.M.; Knight, M.; Evans, W.C.
    The bacterial degradation of flavonoids. Hydroxylation of the A-ring of taxifolin by a soil pseudomonad (1972), Biochem. J., 130, 373-381.
    View publication on PubMedView publication on EuropePMC

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
taxifolin + NADH + O2 Pseudomonas sp.
-
2,3-dihydrogossypetin + NAD+ + H2O
-
?
taxifolin + NADPH + O2 Pseudomonas sp.
-
2,3-dihydrogossypetin + NADP+ + H2O
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Pseudomonas sp.

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
Pseudomonas sp.
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(-)-fustin + NADH + O2
-
Pseudomonas sp. ? + NAD+ + H2O product not identified ?
taxifolin + NADH + O2
-
Pseudomonas sp. 2,3-dihydrogossypetin + NAD+ + H2O
-
?
taxifolin + NADPH + O2
-
Pseudomonas sp. 2,3-dihydrogossypetin + NADP+ + H2O
-
?

Temperature Stability [┬░C]

Temperature Stability Minimum [┬░C] Temperature Stability Maximum [┬░C] Comment Organism
40
-
25% loss of activity in 10 min Pseudomonas sp.
50
-
95% loss of activity in 10 min Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Pseudomonas sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
6
-
20% of maximal activity Pseudomonas sp.

Cofactor

Cofactor Comment Organism Structure
FAD
-
Pseudomonas sp.
NADH twice oxidized compared to NADPH Pseudomonas sp.
NADPH
-
Pseudomonas sp.