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Literature summary for 1.14.13.19 extracted from

  • Hiraga, Y.; Shimada, N.; Nagashima, Y.; Suda, K.; Kanamori, T.; Ishiguro, K.; Sato, Y.; Hirakawa, H.; Sato, S.; Akashi, T.; Tanaka, Y.; Ohta, D.; Aoki, K.; Shibata, D.; Suzuki, H.; Kera, K.
    Identification of a flavin monooxygenase-like flavonoid 8-hydroxylase with gossypetin synthase activity from Lotus japonicus (2021), Plant Cell Physiol., 62, 411-423 .
    View publication on PubMed
Search for more literature for 1.14.13.19

Cloned(Commentary)

Cloned (Comment) Organism
gene LjB13E21 or LjF8H, DNA and amino acid sequence determination and analysis, phylogenetic analysis, functional recombinant enzyme expression in Arabidopsis thaliana strain Col-0 seedlings under the control of a CaMV35S promoter using a pBE2113 vector via transfection method using Agrobacterium tumefaciens strain (GV3101::pMP90), and recombinant expression in Saccharomyces cerevisiae strain BJ2168 microsomes, semi-quantitative RT-PCR enzyme expression analysis Lotus japonicus

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
 Lotus japonicus
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
taxifolin + NADPH + H+ + O2 Lotus japonicus
-
 2,3-dihydrogossypetin + NADP+ + H2O
-
 ?
taxifolin + NADPH + H+ + O2 Lotus japonicus Miyakojima MG-20
-
 2,3-dihydrogossypetin + NADP+ + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Lotus japonicus A0A830U7D1 Lotus corniculatus var. japonicus
-
Lotus japonicus Miyakojima MG-20 A0A830U7D1 Lotus corniculatus var. japonicus
-

Source Tissue

Source Tissue Comment Organism Textmining
flower LjF8H has high relative expression in the early flowering developmental stage Lotus japonicus
-
flower bud
-
 Lotus japonicus
-
additional information LjF8H expression pattern, overview Lotus japonicus
-
petal
-
 Lotus japonicus
-
seedling
-
 Lotus japonicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
apigenin + NADPH + H+ + O2
-
 Lotus japonicus isoscutellarein + NADP+ + H2O
-
 ?
apigenin + NADPH + H+ + O2
-
 Lotus japonicus Miyakojima MG-20 isoscutellarein + NADP+ + H2O
-
 ?
eriodictyol + NADPH + H+ + O2
-
 Lotus japonicus 6-hydroxyeriodictyol + NADP+ + H2O
-
 ?
eriodictyol + NADPH + H+ + O2
-
 Lotus japonicus Miyakojima MG-20 6-hydroxyeriodictyol + NADP+ + H2O
-
 ?
eriodictyol + NADPH + H+ + O2
-
 Lotus japonicus 8-hydroxyeriodictyol + NADP+ + H2O
-
 ?
eriodictyol + NADPH + H+ + O2
-
 Lotus japonicus Miyakojima MG-20 8-hydroxyeriodictyol + NADP+ + H2O
-
 ?
kaempferol + NADPH + H+ + O2
-
 Lotus japonicus herbacetin + NADP+ + H2O
-
 ?
luteolin + NADPH + H+ + O2
-
 Lotus japonicus 8-hydroxyluteolin + NADP+ + H2O
-
 ?
additional information broad substrate acceptance of enzyme LjF8H, overview Lotus japonicus ?
-
-
additional information broad substrate acceptance of enzyme LjF8H, overview Lotus japonicus Miyakojima MG-20 ?
-
-
naringenin + NADPH + H+ + O2
-
 Lotus japonicus isocarthamidin + NADP+ + H2O
-
 ?
quercetin + NADPH + H+ + O2
-
 Lotus japonicus gossypetin + NADP+ + H2O
-
 ?
taxifolin + NADPH + H+ + O2
-
 Lotus japonicus 2,3-dihydrogossypetin + NADP+ + H2O
-
 ?
taxifolin + NADPH + H+ + O2
-
 Lotus japonicus Miyakojima MG-20 2,3-dihydrogossypetin + NADP+ + H2O
-
 ?
taxifolin + NADPH + H+ + O2
-
 Lotus japonicus 6-hydroxytaxifolin + NADP+ + H2O
-
 ?

Synonyms

Synonyms Comment Organism
F8H
-
 Lotus japonicus
flavin monooxygenase-like flavonoid 8-hydroxylase
-
 Lotus japonicus
flavonoid 8-hydroxylase
-
 Lotus japonicus
LjF8H
-
 Lotus japonicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Lotus japonicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Lotus japonicus

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Lotus japonicus
NADPH
-
 Lotus japonicus

General Information

General Information Comment Organism
evolution Lotus japonicus synthesizes and accumulates gossypetin glycosides in flower, thereby impacting on the tone of the yellow color in petals, possible F8H responsible for gossypetin synthesis in petals. Enzyme LjF8H is class-A flavoprotein monooxygenase Lotus japonicus
physiological function enzyme LjF8H has high relative expression in the early flowering developmental stage, implying that LjF8H may also contribute for flower pigment synthesis before flowering Lotus japonicus