Organism | UniProt | Comment | Textmining |
---|---|---|---|
Micromonospora sp. | B5APQ9 | - |
- |
Micromonospora sp. ATCC 39149 | B5APQ9 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
TDP-L-epi-vancosamine + 2 NADPH + 2 H+ + 2 O2 | enzyme catalyzes a two-step-oxidation. For the second oxidation step, three elementary catalytic steps are found, i.e. a hydroxylation step, a hydrogen back-transfer step and a hydroxyl group elimination step. The hydroxylation step is the rate-determining step with an energy barrier of 26.3 kcal/mol. An oxygen atom from the coenzyme FADHOOH is inserted into the product. Groups Gly132/Ala133/Leu134, Met375/Gln376 and a water fence play a key role in facilitating the rate-determining step. Residues Leu160, Val161 and Ser162 are critical to suppress the rate-determining step | Micromonospora sp. | ? + 2 NADP+ + 3 H2O | - |
? | |
TDP-L-epi-vancosamine + 2 NADPH + 2 H+ + 2 O2 | enzyme catalyzes a two-step-oxidation. For the second oxidation step, three elementary catalytic steps are found, i.e. a hydroxylation step, a hydrogen back-transfer step and a hydroxyl group elimination step. The hydroxylation step is the rate-determining step with an energy barrier of 26.3 kcal/mol. An oxygen atom from the coenzyme FADHOOH is inserted into the product. Groups Gly132/Ala133/Leu134, Met375/Gln376 and a water fence play a key role in facilitating the rate-determining step. Residues Leu160, Val161 and Ser162 are critical to suppress the rate-determining step | Micromonospora sp. ATCC 39149 | ? + 2 NADP+ + 3 H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
nitrososynthase | - |
Micromonospora sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | residues L160 ans F374 are involved in binding | Micromonospora sp. |