Cloned (Comment) | Organism |
---|---|
His-tagged fusion protein, expression in Escherichia coli | Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0091 | - |
theophylline | pH 7.5, 30°C | Pseudomonas putida | |
0.037 | - |
Caffeine | pH 7.5, 30°C | Pseudomonas putida | |
0.053 | - |
paraxanthine | pH 7.5, 30°C | Pseudomonas putida | |
0.27 | - |
1-Methylxanthine | pH 7.5, 30°C | Pseudomonas putida |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
non-heme iron | a non-heme iron oxygenase. Contains approximately 2 mol of acid labile sulfur and 2 mol of iron per mol of enzyme monomer | Pseudomonas putida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
x * 40000, SDS-PAGE | Pseudomonas putida |
40200 | - |
x * 40200, calculated from sequence | Pseudomonas putida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
caffeine + O2 + NAD(P)H + H+ | Pseudomonas putida | the enzyme forms part of the degradation pathway of methylxanthines. The activity of the enzyme is dependent on electron transfer from NADH via a redox-center-dense Rieske reductase, NdmD | theobromine + NAD(P)+ + H2O + formaldehyde | - |
? | |
caffeine + O2 + NAD(P)H + H+ | Pseudomonas putida CBB5 | the enzyme forms part of the degradation pathway of methylxanthines. The activity of the enzyme is dependent on electron transfer from NADH via a redox-center-dense Rieske reductase, NdmD | theobromine + NAD(P)+ + H2O + formaldehyde | - |
? | |
paraxanthine + O2 + NAD(P)H + H+ | Pseudomonas putida | the enzyme forms part of the degradation pathway of methylxanthines. The activity of the enzyme is dependent on electron transfer from NADH via a redox-center-dense Rieske reductase, NdmD | 7-methylxanthine + NAD(P)+ + H2O + formaldehyde | - |
? | |
paraxanthine + O2 + NAD(P)H + H+ | Pseudomonas putida CBB5 | the enzyme forms part of the degradation pathway of methylxanthines. The activity of the enzyme is dependent on electron transfer from NADH via a redox-center-dense Rieske reductase, NdmD | 7-methylxanthine + NAD(P)+ + H2O + formaldehyde | - |
? | |
theophylline + O2 + NAD(P)H + H+ | Pseudomonas putida | the enzyme forms part of the degradation pathway of methylxanthines. The activity of the enzyme is dependent on electron transfer from NADH via a redox-center-dense Rieske reductase, NdmD | 3-methylxanthine + NAD(P)+ + H2O + formaldehyde | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | H9N289 | - |
- |
Pseudomonas putida CBB5 | H9N289 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-methylxanthine + O2 + NADH + H+ | - |
Pseudomonas putida | xanthine + NAD+ + H2O + formaldehyde | - |
? | |
caffeine + O2 + NAD(P)H + H+ | the enzyme forms part of the degradation pathway of methylxanthines. The activity of the enzyme is dependent on electron transfer from NADH via a redox-center-dense Rieske reductase, NdmD | Pseudomonas putida | theobromine + NAD(P)+ + H2O + formaldehyde | - |
? | |
caffeine + O2 + NAD(P)H + H+ | the enzyme forms part of the degradation pathway of methylxanthines. The activity of the enzyme is dependent on electron transfer from NADH via a redox-center-dense Rieske reductase, NdmD | Pseudomonas putida CBB5 | theobromine + NAD(P)+ + H2O + formaldehyde | - |
? | |
caffeine + O2 + NADH + H+ | the activity of the enzyme is dependent on electron transfer from NADH via a redox-center-dense Rieske reductase, NdmD | Pseudomonas putida | theobromine + NAD+ + H2O + formaldehyde | - |
? | |
caffeine + O2 + NADH + H+ | the activity of the enzyme is dependent on electron transfer from NADH via a redox-center-dense Rieske reductase, NdmD | Pseudomonas putida CBB5 | theobromine + NAD+ + H2O + formaldehyde | - |
? | |
additional information | no activity with theobromine, 3-methylxanthine and 7-methylxanthine | Pseudomonas putida | ? | - |
? | |
additional information | no activity with theobromine, 3-methylxanthine and 7-methylxanthine | Pseudomonas putida CBB5 | ? | - |
? | |
paraxanthine + O2 + NAD(P)H + H+ | the enzyme forms part of the degradation pathway of methylxanthines. The activity of the enzyme is dependent on electron transfer from NADH via a redox-center-dense Rieske reductase, NdmD | Pseudomonas putida | 7-methylxanthine + NAD(P)+ + H2O + formaldehyde | - |
? | |
paraxanthine + O2 + NAD(P)H + H+ | the enzyme forms part of the degradation pathway of methylxanthines. The activity of the enzyme is dependent on electron transfer from NADH via a redox-center-dense Rieske reductase, NdmD | Pseudomonas putida CBB5 | 7-methylxanthine + NAD(P)+ + H2O + formaldehyde | - |
? | |
paraxanthine + O2 + NADH + H+ | - |
Pseudomonas putida | 7-methylxanthine + NAD+ + H2O + formaldehyde | - |
? | |
theophylline + O2 + NAD(P)H + H+ | the enzyme forms part of the degradation pathway of methylxanthines. The activity of the enzyme is dependent on electron transfer from NADH via a redox-center-dense Rieske reductase, NdmD | Pseudomonas putida | 3-methylxanthine + NAD(P)+ + H2O + formaldehyde | - |
? | |
theophylline + O2 + NADH + H+ | - |
Pseudomonas putida | 3-methylxanthine + NAD+ + H2O + formaldehyde | - |
? | |
theophylline + O2 + NADH + H+ | - |
Pseudomonas putida CBB5 | 3-methylxanthine + NAD+ + H2O + formaldehyde | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 40000, SDS-PAGE | Pseudomonas putida |
? | x * 40200, calculated from sequence | Pseudomonas putida |
Synonyms | Comment | Organism |
---|---|---|
ndmA | - |
Pseudomonas putida |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Pseudomonas putida |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.27 | - |
1-Methylxanthine | pH 7.5, 30°C | Pseudomonas putida | |
1.38 | - |
theophylline | pH 7.5, 30°C | Pseudomonas putida | |
2.17 | - |
paraxanthine | pH 7.5, 30°C | Pseudomonas putida | |
3.17 | - |
Caffeine | pH 7.5, 30°C | Pseudomonas putida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | electron transfer from NADH via a redox-center-dense Rieske reductase, NdmD | Pseudomonas putida |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme forms part of the degradation pathway of methylxanthines | Pseudomonas putida |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1 | - |
1-Methylxanthine | pH 7.5, 30°C | Pseudomonas putida | |
40.9 | - |
paraxanthine | pH 7.5, 30°C | Pseudomonas putida | |
85.7 | - |
Caffeine | pH 7.5, 30°C | Pseudomonas putida | |
151.6 | - |
theophylline | pH 7.5, 30°C | Pseudomonas putida |