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Literature summary for 1.14.13.178 extracted from

  • Summers, R.M.; Louie, T.M.; Yu, C.L.; Gakhar, L.; Louie, K.C.; Subramanian, M.
    Novel, highly specific N-demethylases enable bacteria to live on caffeine and related purine alkaloids (2012), J. Bacteriol., 194, 2041-2049.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
His-tagged fusion protein, expression in Escherichia coli Pseudomonas putida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0091
-
theophylline pH 7.5, 30°C Pseudomonas putida
0.037
-
Caffeine pH 7.5, 30°C Pseudomonas putida
0.053
-
paraxanthine pH 7.5, 30°C Pseudomonas putida
0.27
-
1-Methylxanthine pH 7.5, 30°C Pseudomonas putida

Metals/Ions

Metals/Ions Comment Organism Structure
non-heme iron a non-heme iron oxygenase. Contains approximately 2 mol of acid labile sulfur and 2 mol of iron per mol of enzyme monomer Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
x * 40000, SDS-PAGE Pseudomonas putida
40200
-
x * 40200, calculated from sequence Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
caffeine + O2 + NAD(P)H + H+ Pseudomonas putida the enzyme forms part of the degradation pathway of methylxanthines. The activity of the enzyme is dependent on electron transfer from NADH via a redox-center-dense Rieske reductase, NdmD theobromine + NAD(P)+ + H2O + formaldehyde
-
?
caffeine + O2 + NAD(P)H + H+ Pseudomonas putida CBB5 the enzyme forms part of the degradation pathway of methylxanthines. The activity of the enzyme is dependent on electron transfer from NADH via a redox-center-dense Rieske reductase, NdmD theobromine + NAD(P)+ + H2O + formaldehyde
-
?
paraxanthine + O2 + NAD(P)H + H+ Pseudomonas putida the enzyme forms part of the degradation pathway of methylxanthines. The activity of the enzyme is dependent on electron transfer from NADH via a redox-center-dense Rieske reductase, NdmD 7-methylxanthine + NAD(P)+ + H2O + formaldehyde
-
?
paraxanthine + O2 + NAD(P)H + H+ Pseudomonas putida CBB5 the enzyme forms part of the degradation pathway of methylxanthines. The activity of the enzyme is dependent on electron transfer from NADH via a redox-center-dense Rieske reductase, NdmD 7-methylxanthine + NAD(P)+ + H2O + formaldehyde
-
?
theophylline + O2 + NAD(P)H + H+ Pseudomonas putida the enzyme forms part of the degradation pathway of methylxanthines. The activity of the enzyme is dependent on electron transfer from NADH via a redox-center-dense Rieske reductase, NdmD 3-methylxanthine + NAD(P)+ + H2O + formaldehyde
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas putida H9N289
-
-
Pseudomonas putida CBB5 H9N289
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-methylxanthine + O2 + NADH + H+
-
Pseudomonas putida xanthine + NAD+ + H2O + formaldehyde
-
?
caffeine + O2 + NAD(P)H + H+ the enzyme forms part of the degradation pathway of methylxanthines. The activity of the enzyme is dependent on electron transfer from NADH via a redox-center-dense Rieske reductase, NdmD Pseudomonas putida theobromine + NAD(P)+ + H2O + formaldehyde
-
?
caffeine + O2 + NAD(P)H + H+ the enzyme forms part of the degradation pathway of methylxanthines. The activity of the enzyme is dependent on electron transfer from NADH via a redox-center-dense Rieske reductase, NdmD Pseudomonas putida CBB5 theobromine + NAD(P)+ + H2O + formaldehyde
-
?
caffeine + O2 + NADH + H+ the activity of the enzyme is dependent on electron transfer from NADH via a redox-center-dense Rieske reductase, NdmD Pseudomonas putida theobromine + NAD+ + H2O + formaldehyde
-
?
caffeine + O2 + NADH + H+ the activity of the enzyme is dependent on electron transfer from NADH via a redox-center-dense Rieske reductase, NdmD Pseudomonas putida CBB5 theobromine + NAD+ + H2O + formaldehyde
-
?
additional information no activity with theobromine, 3-methylxanthine and 7-methylxanthine Pseudomonas putida ?
-
?
additional information no activity with theobromine, 3-methylxanthine and 7-methylxanthine Pseudomonas putida CBB5 ?
-
?
paraxanthine + O2 + NAD(P)H + H+ the enzyme forms part of the degradation pathway of methylxanthines. The activity of the enzyme is dependent on electron transfer from NADH via a redox-center-dense Rieske reductase, NdmD Pseudomonas putida 7-methylxanthine + NAD(P)+ + H2O + formaldehyde
-
?
paraxanthine + O2 + NAD(P)H + H+ the enzyme forms part of the degradation pathway of methylxanthines. The activity of the enzyme is dependent on electron transfer from NADH via a redox-center-dense Rieske reductase, NdmD Pseudomonas putida CBB5 7-methylxanthine + NAD(P)+ + H2O + formaldehyde
-
?
paraxanthine + O2 + NADH + H+
-
Pseudomonas putida 7-methylxanthine + NAD+ + H2O + formaldehyde
-
?
theophylline + O2 + NAD(P)H + H+ the enzyme forms part of the degradation pathway of methylxanthines. The activity of the enzyme is dependent on electron transfer from NADH via a redox-center-dense Rieske reductase, NdmD Pseudomonas putida 3-methylxanthine + NAD(P)+ + H2O + formaldehyde
-
?
theophylline + O2 + NADH + H+
-
Pseudomonas putida 3-methylxanthine + NAD+ + H2O + formaldehyde
-
?
theophylline + O2 + NADH + H+
-
Pseudomonas putida CBB5 3-methylxanthine + NAD+ + H2O + formaldehyde
-
?

Subunits

Subunits Comment Organism
? x * 40000, SDS-PAGE Pseudomonas putida
? x * 40200, calculated from sequence Pseudomonas putida

Synonyms

Synonyms Comment Organism
ndmA
-
Pseudomonas putida

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Pseudomonas putida

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.27
-
1-Methylxanthine pH 7.5, 30°C Pseudomonas putida
1.38
-
theophylline pH 7.5, 30°C Pseudomonas putida
2.17
-
paraxanthine pH 7.5, 30°C Pseudomonas putida
3.17
-
Caffeine pH 7.5, 30°C Pseudomonas putida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Pseudomonas putida

Cofactor

Cofactor Comment Organism Structure
NADH electron transfer from NADH via a redox-center-dense Rieske reductase, NdmD Pseudomonas putida

General Information

General Information Comment Organism
physiological function the enzyme forms part of the degradation pathway of methylxanthines Pseudomonas putida

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1
-
1-Methylxanthine pH 7.5, 30°C Pseudomonas putida
40.9
-
paraxanthine pH 7.5, 30°C Pseudomonas putida
85.7
-
Caffeine pH 7.5, 30°C Pseudomonas putida
151.6
-
theophylline pH 7.5, 30°C Pseudomonas putida