Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.14.13.172 extracted from

  • Zhou, N.Y.; Al-Dulayymi, J.; Baird, M.S.; Williams, P.A.
    Salicylate 5-hydroxylase from Ralstonia sp. strain U2: a monooxygenase with close relationships to and shared electron transport proteins with naphthalene dioxygenase (2002), J. Bacteriol., 184, 1547-1555.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.13.172

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3)/pLysS cells Ralstonia sp.

Organism

Organism UniProt Comment Textmining
Ralstonia sp. O52379 and O52380 large oxygenase component and small oxagenase component
-
Ralstonia sp. U2 O52379 and O52380 large oxygenase component and small oxagenase component
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.051
-
 in 50 mM MES buffer, pH 6.4, at 30°C Ralstonia sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no activity is detected against 2-hydroxycinnamate, 3-hydroxycinnamate, 2-hydroxyphenylacetate, 3-hydroxyphenylacetate, 2-hydroxybenzophenone, 1-hydroxy-2-naphthoate, 4-methoxysalicylate, and 2-hydroxyacetophenone Ralstonia sp. ?
-
 ?
additional information no activity is detected against 2-hydroxycinnamate, 3-hydroxycinnamate, 2-hydroxyphenylacetate, 3-hydroxyphenylacetate, 2-hydroxybenzophenone, 1-hydroxy-2-naphthoate, 4-methoxysalicylate, and 2-hydroxyacetophenone Ralstonia sp. U2 ?
-
 ?
salicylate + NADH + H+ + O2
-
 Ralstonia sp. 2,5-dihydroxybenzoate + NAD+ + H2O
-
 ?
salicylate + NADH + H+ + O2
-
 Ralstonia sp. U2 2,5-dihydroxybenzoate + NAD+ + H2O
-
 ?

Synonyms

Synonyms Comment Organism
NagGH
-
 Ralstonia sp.
S5H a three-component system consisting of NagGH (the oxygenase components), NagAa (ferredoxin reductase), and NagAb (ferredoxin) Ralstonia sp.

Cofactor

Cofactor Comment Organism Structure
NADH
-
 Ralstonia sp.