Cloned (Comment) | Organism |
---|---|
gene YUC2, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-chlorophenylboronic acid | moderate inhibition of enzyme activity and primary root elongation. Probably unspecific inhibition | Arabidopsis thaliana | |
3,5-dibromophenylboronic acid | probably unspecific inhibition, inhibits root hair formation in wild-type Arabidopsis thaliana seedlings | Arabidopsis thaliana | |
3,5-dichlorophenylboronic acid | probably unspecific inhibition, inhibits root hair formation in wild-type Arabidopsis thaliana seedlings | Arabidopsis thaliana | |
3-chlorophenylboronic acid | probably unspecific inhibition, strongly inhibits root hair formation in wild-type Arabidopsis thaliana seedlings | Arabidopsis thaliana | |
4-biphenylboronic acid | BBo, competitive, inhibits the activity of recombinant YUC in vitro, reduces endogenous indole-3-acetic acid content, and strongly inhibits primary root elongation and lateral root formation in wild-type Arabidopsis thaliana seedlings | Arabidopsis thaliana | |
4-chlorophenylboronic acid | probably unspecific inhibition, strongly inhibits root hair formation in wild-type Arabidopsis thaliana seedlings | Arabidopsis thaliana | |
4-methyl-4-biphenylboronic acid | weak inhibition of enzyme activity, but strong inhibition of primary root elongation | Arabidopsis thaliana | |
4-phenoxyphenylboronic acid | PPBo, competitive, inhibits the activity of recombinant YUC in vitro, reduces endogenous indole-3-acetic acid content, and strongly inhibits primary root elongation and lateral root formation in wild-type Arabidopsis thaliana seedlings. The compound does not interfere with the auxin response of auxin-marker genes when it was co-treated with indole acetic acid, suggesting that PPBo is not an inhibitor of auxin sensing or signaling | Arabidopsis thaliana | |
additional information | FAD and boronic acid is not the main reason for inhibition of enzyme activity by boronic acid derivatives. Poor inhibition by 2,6-dichlorophenylboronic acid | Arabidopsis thaliana | |
phenethylboronic acid | moderate inhibition of enzyme activity and primary root elongation | Arabidopsis thaliana | |
phenylboronic acid | PBo, inhibition of enzyme activity, but not of primary root elongation | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(indol-3-yl)pyruvate + NADPH + H+ + O2 | Arabidopsis thaliana | - |
(indol-3-yl)acetate + NADP+ + H2O + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q9SVQ1 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Arabidopsis thaliana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seedling | - |
Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(indol-3-yl)pyruvate + NADPH + H+ + O2 | - |
Arabidopsis thaliana | (indol-3-yl)acetate + NADP+ + H2O + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
flavin-containing monooxygenase YUC | - |
Arabidopsis thaliana |
YUC2 | - |
Arabidopsis thaliana |
YUCCA | - |
Arabidopsis thaliana |
YUCCA2 | - |
Arabidopsis thaliana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
assay at | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
assay at | Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Arabidopsis thaliana | |
NADPH | - |
Arabidopsis thaliana |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000056 | - |
4-phenoxyphenylboronic acid | pH 8.5, 35°C, recombinant His-tagged enzyme | Arabidopsis thaliana | |
0.000067 | - |
4-biphenylboronic acid | pH 8.5, 35°C, recombinant His-tagged enzyme | Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
metabolism | YUCCA, a flavin-containing monooxygenase (YUC), catalyzes the last step of conversion from indole-3-pyruvate to indole-3-acetic acid | Arabidopsis thaliana |
physiological function | the enzyme catalzes the last step in the indole-3-pyruvate pathway, a major biosynthesis pathway in Arabidopsis thaliana for indole-3-acetic acid, the most common member of the auxin family | Arabidopsis thaliana |