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Literature summary for 1.14.13.168 extracted from
- Small-molecule auxin inhibitors that target YUCCA are powerful tools for studying auxin function (2015), Plant J., 84, 827-837 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene YUC2, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Arabidopsis thaliana |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-chlorophenylboronic acid | moderate inhibition of enzyme activity and primary root elongation. Probably unspecific inhibition | Arabidopsis thaliana |  |
| 3,5-dibromophenylboronic acid | probably unspecific inhibition, inhibits root hair formation in wild-type Arabidopsis thaliana seedlings | Arabidopsis thaliana | |
| 3,5-dichlorophenylboronic acid | probably unspecific inhibition, inhibits root hair formation in wild-type Arabidopsis thaliana seedlings | Arabidopsis thaliana | |
| 3-chlorophenylboronic acid | probably unspecific inhibition, strongly inhibits root hair formation in wild-type Arabidopsis thaliana seedlings | Arabidopsis thaliana | |
| 4-biphenylboronic acid | BBo, competitive, inhibits the activity of recombinant YUC in vitro, reduces endogenous indole-3-acetic acid content, and strongly inhibits primary root elongation and lateral root formation in wild-type Arabidopsis thaliana seedlings | Arabidopsis thaliana | |
| 4-chlorophenylboronic acid | probably unspecific inhibition, strongly inhibits root hair formation in wild-type Arabidopsis thaliana seedlings | Arabidopsis thaliana | |
| 4-methyl-4-biphenylboronic acid | weak inhibition of enzyme activity, but strong inhibition of primary root elongation | Arabidopsis thaliana | |
| 4-phenoxyphenylboronic acid | PPBo, competitive, inhibits the activity of recombinant YUC in vitro, reduces endogenous indole-3-acetic acid content, and strongly inhibits primary root elongation and lateral root formation in wild-type Arabidopsis thaliana seedlings. The compound does not interfere with the auxin response of auxin-marker genes when it was co-treated with indole acetic acid, suggesting that PPBo is not an inhibitor of auxin sensing or signaling | Arabidopsis thaliana | |
| additional information | FAD and boronic acid is not the main reason for inhibition of enzyme activity by boronic acid derivatives. Poor inhibition by 2,6-dichlorophenylboronic acid | Arabidopsis thaliana | |
| phenethylboronic acid | moderate inhibition of enzyme activity and primary root elongation | Arabidopsis thaliana | |
| phenylboronic acid | PBo, inhibition of enzyme activity, but not of primary root elongation | Arabidopsis thaliana | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (indol-3-yl)pyruvate + NADPH + H+ + O2 | Arabidopsis thaliana | - |
(indol-3-yl)acetate + NADP+ + H2O + CO2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | Q9SVQ1 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Arabidopsis thaliana |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seedling | - |
Arabidopsis thaliana | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (indol-3-yl)pyruvate + NADPH + H+ + O2 | - |
Arabidopsis thaliana | (indol-3-yl)acetate + NADP+ + H2O + CO2 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| flavin-containing monooxygenase YUC | - |
Arabidopsis thaliana |
| YUC2 | - |
Arabidopsis thaliana |
| YUCCA | - |
Arabidopsis thaliana |
| YUCCA2 | - |
Arabidopsis thaliana |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
assay at | Arabidopsis thaliana |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
assay at | Arabidopsis thaliana |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | - |
Arabidopsis thaliana | |
| NADPH | - |
Arabidopsis thaliana | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.000056 | - |
4-phenoxyphenylboronic acid | pH 8.5, 35°C, recombinant His-tagged enzyme | Arabidopsis thaliana | |
| 0.000067 | - |
4-biphenylboronic acid | pH 8.5, 35°C, recombinant His-tagged enzyme | Arabidopsis thaliana | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | YUCCA, a flavin-containing monooxygenase (YUC), catalyzes the last step of conversion from indole-3-pyruvate to indole-3-acetic acid | Arabidopsis thaliana |
| physiological function | the enzyme catalzes the last step in the indole-3-pyruvate pathway, a major biosynthesis pathway in Arabidopsis thaliana for indole-3-acetic acid, the most common member of the auxin family | Arabidopsis thaliana |
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Release 2023.1 (January 2023)
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