Cloned (Comment) | Organism |
---|---|
His-tagged enzyme is expressed in Escherichia coli BL21(DE3) cells | Pseudomonas sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | addition of Mg2+ or Mn2+ ions has no effect on the activity | Pseudomonas sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
47000 | - |
1 * 47000, His-tagged enzyme, gel filtration | Pseudomonas sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | C1I201 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Pseudomonas sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture condition:4-nitrophenol-grown cell | - |
Pseudomonas sp. | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.9 | - |
at pH 7.0 and 30°C | Pseudomonas sp. |
Storage Stability | Organism |
---|---|
-20°C, without glycerol, rapid inactivation | Pseudomonas sp. |
-70°C, in 50% (v/v) glycerol, 7 days, 70% loss of activity | Pseudomonas sp. |
0°C, storage medium, no loss of activity after several hours, 30% loss of activity after 12 h, 80% loss of activity after 40 h | Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrocatechol + NADPH + H+ + O2 | - |
Pseudomonas sp. | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 47000, His-tagged enzyme, gel filtration | Pseudomonas sp. |
Synonyms | Comment | Organism |
---|---|---|
para-nitrophenol 4-monooxygenase | - |
Pseudomonas sp. |
PNP 4-monooxygenase | - |
Pseudomonas sp. |
pnpA | - |
Pseudomonas sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | dependent on, negligible activity is observed when FMN replaces FAD | Pseudomonas sp. | |
NADH | NADPH can be replaced by NADH with the same efficiency | Pseudomonas sp. | |
NADPH | - |
Pseudomonas sp. |