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Literature summary for 1.14.13.163 extracted from

  • Tang, H.; Yao, Y.; Zhang, D.; Meng, X.; Wang, L.; Yu, H.; Ma, L.; Xu, P.
    A novel NADH-dependent and FAD-containing hydroxylase is crucial for nicotine degradation by Pseudomonas putida (2011), J. Biol. Chem., 286, 39179-39187.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.13.163

Activating Compound

Activating Compound Comment Organism Structure
6-hydroxy-3-succinoylpyridine acts as an initial effector and strongly stimulates NADH oxidation Pseudomonas putida

Protein Variants

Protein Variants Comment Organism
additional information deletion of a GADGA motif in the FAD binding-domain results in complete loss of activity in the mutant protein Pseudomonas putida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.104
-
 4-(6-hydroxypyridin-3-yl)-4-oxobutanoate pH 8.0, 25°C Pseudomonas putida
0.175
-
 NADH pH 8.0, 25°C Pseudomonas putida

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ 2 mM, complete loss of activity Pseudomonas putida
Zn2+ 2 mM, complete loss of activity Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 2 * 40000, SDS-PAGE Pseudomonas putida
80000
-
 PAGE Pseudomonas putida

Organism

Organism UniProt Comment Textmining
Pseudomonas putida F8G0M4
-
-
Pseudomonas putida S16 F8G0M4
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas putida

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.8
-
 pH 8.0, 25°C Pseudomonas putida

Storage Stability

Storage Stability Organism
-20°C, Tris-HCl buffer, stable for several months Pseudomonas putida
4°C Tris-HCl buffer, stable for at least one week Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-(6-hydroxypyridin-3-yl)-4-oxobutanoate + 2 NADH + 2 H+ + O2
-
 Pseudomonas putida 2,5-dihydroxypyridine + succinate semialdehyde + 2 NAD+ + H2O
-
 ?
4-(6-hydroxypyridin-3-yl)-4-oxobutanoate + 2 NADH + 2 H+ + O2
-
 Pseudomonas putida S16 2,5-dihydroxypyridine + succinate semialdehyde + 2 NAD+ + H2O
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 40000, SDS-PAGE Pseudomonas putida

Synonyms

Synonyms Comment Organism
HSP hydroxylase
-
 Pseudomonas putida
hspB
-
 Pseudomonas putida

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
-
 Pseudomonas putida

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25
-
 30 min, stable up to Pseudomonas putida

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2
-
 NADH pH 8.0, 25°C Pseudomonas putida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Pseudomonas putida

pH Stability

pH Stability pH Stability Maximum Comment Organism
7 9 incubation overnight, 20% loss of activity Pseudomonas putida

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Pseudomonas putida
additional information no cofactor: NADPH Pseudomonas putida
NADH specific for NADH Pseudomonas putida

General Information

General Information Comment Organism
physiological function gene is essential for nicotine degradation Pseudomonas putida