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Literature summary for 1.14.13.154 extracted from
- Overproduction and characterization of the erythromycin C-12 hydroxylase, EryK (1995), Biochemistry, 34, 1858-1866.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overproduction of EryK in Escherichia coli as insoluble inclusion bodies | Saccharopolyspora erythraea |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| erythromycin D | substrate inhibition | Saccharopolyspora erythraea |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.008 | - |
erythromycin D | pH 7.5, 30°C | Saccharopolyspora erythraea | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| erythromycin D + NADPH + H+ + O2 | Saccharopolyspora erythraea | - |
erythromycin C + NADP+ + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharopolyspora erythraea | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Saccharopolyspora erythraea |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| erythromycin B + NADPH + H+ + O2 | 1200-1900-fold preference for erythromycin D over the alternative C-12 hydroxylase substrate erythromycin B | Saccharopolyspora erythraea | erythromycin A + NADP+ + H2O | - |
? | |
| erythromycin D + NADPH + H+ + O2 | - |
Saccharopolyspora erythraea | erythromycin C + NADP+ + H2O | - |
? | |
| erythromycin D + NADPH + H+ + O2 | 1200-1900-fold preference for erythromycin D over the alternative C-12 hydroxylase substrate erythromycin B | Saccharopolyspora erythraea | erythromycin C + NADP+ + H2O | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| EryK | - |
Saccharopolyspora erythraea |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Saccharopolyspora erythraea |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.8 | - |
erythromycin D | pH 7.5, 30°C | Saccharopolyspora erythraea | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Saccharopolyspora erythraea |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| cytochrome P-450 | a heme-thiolate protein (P-450) | Saccharopolyspora erythraea | |
| NADPH | - |
Saccharopolyspora erythraea | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0166 | - |
erythromycin D | pH 7.5, 30°C | Saccharopolyspora erythraea | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 225 | - |
erythromycin D | pH 7.5, 30°C | Saccharopolyspora erythraea | |
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